Scavenging of Reactive Oxygen Species by Eriobotrya japonica Seed Extract

Abstract

We have clarified that Eriobotrya japonica seed extract has strong antioxidative activity, and is effective for the prevention and treatment of various diseases, such as hepatopathy and nephropathy. In this study, to investigate the influences of components of Eriobotrya japonica seed extract on its antioxidative activity, extracts were prepared using various solvents (n-hexane (Hex), ethyl acetate (EtOAc), n-butanol (n-BuOH), methanol (MeOH) and H 2 O) and the antioxidative activity of the solvent fractions and components was evaluated based on the scavenging of various radicals (DPPH and O 2 ؊ ) measured by the ESR method and the inhibition of Fe 3؉ -ADP induced NADPH dependent lipid peroxidation in rat liver microsomes. The radical scavenging activities and inhibitory activities on lipid peroxidation differed among the solvent fractions and components. In the n-BuOH, MeOH and H 2 O fractions, radical scavenging activity and inhibitory activity on lipid peroxidation were high. In addition, these fractions contained abundant polyphenols, and the radical scavenging activity increased with the polyphenol content. In the low-polar Hex and EtOAc fractions, the radical scavenging activity was low, but the lipid peroxidation inhibition activity was high. These fractions contained b b-sitosterol, and the inhibitory activity on lipid peroxidation was high. Based on these findings, the antioxidative activity of Eriobotrya japonica seed extract may be derived from many components involved in a complex mechanism, resulting in high activity.

Antioxidative activity of Ginkgo, Echinacea, and Ginseng tinctures

“…According to literature data, phenol compounds in plants are the largest group of compounds with antioxidative properties (10,14). For this reason we found it expedient to determine the total amount of phenol compounds in the investigated tinctures (Fig.…”

Effects of Eriobotrya japonica (Lindl.) flower extracts on mercuric chloride-induced hepatotoxicity in rats

“…There is a relationship between antioxidant activities and the contents of total phenolics or flavonoids. Also, Yokota et al [34] reported scavenging activity of ROS by Eriobotrya japonica seed extracts. Scavenging of H 2 O 2 by EJFE may be attributed to their phenolic compounds (gallic acid) which can donate electrons to H 2 O 2 , neutralizing it to H 2 O and O 2 .…”

“…Eriobotrya japonica flower extracts as an antioxidant agent ameliorated oxidative stress in these tissues (see Figure 2). Based on research of Yokota et al [34] Eriobotrya japonica seed extracts have strong antioxidative activities, lipid peroxidation inhibition activities, and are effective for the prevention and treatment of various diseases such as hepatopathy and nephropathy. Also, incubation with ethanol extract of loquat (Eriobotrya japonica) seeds significantly lowered the TBARS levels [36].…”

Phytochemical Screening and Renal Effects of Ethanolic Extract of Eriobotrya Japonica Fruits and Seeds in Alloxan Induced Diabetic Rats

“…Antidiabetic activity has been reported from the leaves of this plant. [11][12][13][14][15][16] . Very less work has been done on the fruit and seeds of this plant.…”