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Peyghan, Rahim; Takamy, G. Azary

doi:10.1023/a:1022408529458

Cited by 79 publications

(17 citation statements)

References 13 publications

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“…In order to measure the cortisol value, ELISA kit (DRG Diagnostics, Mountainside, NJ, USA) was used . ALT, AST and ALP values were determined by the method described by Peyghan and Takamy (2002).…”

Section: Estimation Of Plasma Glucose Cortisol Alp Ast and Altmentioning

confidence: 99%

Analysis of primary and secondary stress responses in bighead carp (Hypophthalmichthys nobilis) by anesthetization with 2-phenoxyethanol

Akbary

Pirbeigi

Jahanbakhshi

2016

Int. J. Environ. Sci. Technol.

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The main objective of the present study was to shed light on the effects of 2-phenoxyethanol on possible primary (cortisol level) and secondary (hematological indices and glucose level) stress responses and changes in the activity of metabolic enzymes (AST, ALT and ALP) in bighead carp (Hypophthalmichthys nobilis). The fish were exposed to 0.1, 0.3, 0.5, 0.7 and 0.9 ml L -1 of 2-phenoxyethanol. 2-Phenoxyethanol induction and recovery times were 115-595 and 29.66-179.3 s, respectively. At a concentration of 0.1 ml L -1 , the anesthetic failed to induce deep anesthesia. The lowest effective concentration of 2-phenoxyethanol was determined to be 0.7 ml L -1 , whereas 0.9 ml L -1 was found to be the most effective one. Data showed that RBC, WBC, hemoglobin and hematocrit values were significantly high in some treatments compared to the control. MCV and MCH contents decreased significantly. MCHC, ALP, AST and ALT did not vary significantly. Plasma glucose and cortisol levels were significantly high compared to the control (in some doses). Moreover, Na ? , K ? , Ca 2? , Mg 2? and Cl -did not vary significantly. Findings suggest that blood parameters were affected by anesthetizing bighead with 2-phenoxyethanol at 0.9 ml L -1 to the lowest extent.

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Section: Estimation Of Plasma Glucose Cortisol Alp Ast and Altmentioning

confidence: 99%

Analysis of primary and secondary stress responses in bighead carp (Hypophthalmichthys nobilis) by anesthetization with 2-phenoxyethanol

Akbary

Pirbeigi

Jahanbakhshi

2016

Int. J. Environ. Sci. Technol.

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“…Ionic ammonium (NH 4 + ) is hydrated and less toxic than molecular ammonia (NH 3 º), which is fat-soluble and thus can penetrate the body of biofilms in turn affecting economic value of aquatic animals [7,9,10]. Accumulation of ammonia in the system could lead to "acute ammonia intoxication" by disrupting the proton gradients and the central nervous system (CNS); besides, loss of equilibrium, hyperexcitability, decreased oxygen-carrying capacity, thickening of mucous cells, liver tissue edema, and in extremis, convulsions, coma, and death [11][12][13]. Thus, the removal of ammonium compounds from the water ecosystems is essential for animal culture applications.…”

Section: Introductionmentioning

confidence: 99%

Bacillus velezensis LG37: Transcriptome profiling and functional verification of GlnK and NmtA in ammonia (NH4+) assimilation

Liu

Babu

Dong

et al. 2020

Preprint

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Background In recent years, interest in Bacillus velezensis has increased significantly due to its role in many industrial water bioremediation processes, including probiotics. In this study, we isolated and assessed the transcriptome of Bacillus velezensis LG37 (isolated from aquaculture pond) under different nitrogen sources. Since Bacillus species exhibit heterogeneity, it is worth to investigate the molecular information of LG37 through ammonia nitrogen assimilation, where ammonia’s nitrogen is considered toxic to aquatics. Results Here, a total of 812 differentially expressed genes (DEGs) from the transcriptomic sequencing of LG37 grown minimal medium supplemented with ammonia (treatment) or glutamine (control) were obtained, from which 56 had Fold Change ≥ 2. BLAST NCBI and UniProt databases revealed 27 out of the 56 DEGs were potentially involved in NH 4 + assimilation. Among them, 8 DEGs together with the two-component regulatory system glnK/glnL were randomly selected for validation by quantitative real-time RT-PCR, and the relative expression level showed that glnK/glnL were significantly upregulated that were consistent with the RNA-seq data coupled with six other genes. Moreover, the transcriptome and relative expression analysis showed that the transporter ( amtB ) gene of LG37 was not involved in ammonia transport even in the highest ammonia concentrations. Besides, CRISPR-Cas9 knockout and overexpression LG37 mutants of glnK further evidenced the exclusion of amtB regulation, suggesting involvement of alternative transporter. Additionally, in the transcriptomic data, a novel ammonium transporter nmtA was expressed significantly in increased ammonia concentrations. Subsequently, OE nmtA and Δ nmtA LG37 strains showed a unique expression pattern compared to that of wild-LG37 strain. Conclusion Based on the transcriptome data, regulation of nitrogen related genes were promptly screened from the newly isolated LG37 strain to analyse the key regulating factors during ammonia assimilation. The novel NmtA transporter of LG37 became apparent in ammonia transport instead of AmtB, which transport ammonium nitrogen in other Bacillus strains, using the genomic editing tools. Collectively, this study defines the heterogeneity of B. velezensis LG37 all alone through comprehensive transcriptome analysis subsequently by genome editing techniques, sheds light on the enigmatic mechanisms under different nitrogen sources also reveal the need for further research.

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“…Ionic ammonium (NH 4 + ) exist in hydrated form is less toxic, whereas the molecular ammonia (NH 3 º) is a fat-soluble substance that can penetrate through the body of biofilms which is highly toxic to the economic aquatic animals [7,9,10]. Accumulation of ammonia in the system could lead to "acute ammonia intoxication" by disrupting the proton gradients and the central nervous system (CNS), besides, loss of equilibrium, hyperexcitability, decreased oxygen-carrying capacity, thickening of mucous cells, liver tissue edema, and in extremis, convulsions, coma, and death [11][12][13]. Thus, removal of ammonia compounds from the water ecosystems is essential for animal culture application.…”

Section: Introductionmentioning

confidence: 99%

Bacillus velezensis LG37: Transcriptome profiling and functional verification of GlnK and NmtA in ammonia (NH4+) assimilation

Liu

Babu

Dong

et al. 2019

Preprint

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Background In recent years, interest in Bacillus velezensis has increased considerably due to its ability in many industrial water treatment bioremediation processes that including aquaculture practice as probiotics. In this study, we isolated and assessed the transcriptome genes expression levels under different nitrogen source from a new Bacillus velezensis LG37 strain isolated from the aquaculture pond water. Since the Bacillus species are determined to exhibit heterogeneity, and the underlying molecular mechanisms remain poorly understood in ammonia nitrogen assimilation, where ammonia nitrogen considered as toxic to aquatics.Results Here, a total of 812 differentially expressed genes (DEGs) from the transcriptomic sequencing of LG37 grown with ammonia or glutamine, from which 56 genes obtained with Fold Change ≥ 2 through BLAST NCBI and UniProt database that displayed 27 DEGs potentially involved in NH4+ assimilation. Among them, the relative expression level of the two-component regulatory system glnK/glnL were significantly upregulated in the transcriptomic profile, and the same was authenticated for their consistency by RT-qPCR along with six other genes. In addition, the transcriptome and relative expression analysis showed that the transporter (amtB) gene of LG37 was not in favor of ammonia transport even in the highest ammonia concentrations. Besides, CRISPR-Cas9 knockout (Δ) and overexpression (OE) LG37 mutants of glnK evidenced the exclusion of amtB regulation, suggesting an involvement of alternative transporter. Moreover, the transcriptomic data exhibited a novel ammonium transporter nmtA that expressed significantly in the expanding ammonia concentrations. Subsequently, OEnmtA and ΔnmtA LG37 strains showed a unique expression pattern compared to that of wild-LG37 strain.Conclusion Based on the transcriptome data, regulation of nitrogen related genes were promptly screened from the newly isolated LG37 strain to analyse the key regulating factors during ammonia assimilation. The novel NmtA transporter of LG37 became apparent in ammonia transport instead of AmtB, which transport ammonium nitrogen in other Bacillus strains, with the aid of the genomic editing tools. The validated NmtA and other transcriptome predicted data would shed a new light on the assimilation mechanism of ammonia, and the isolate can be broadly applicable to facilitate the removal of ammonium nitrogen.

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Cited by 79 publications

References 13 publications

Analysis of primary and secondary stress responses in bighead carp (Hypophthalmichthys nobilis) by anesthetization with 2-phenoxyethanol

Analysis of primary and secondary stress responses in bighead carp (Hypophthalmichthys nobilis) by anesthetization with 2-phenoxyethanol

Bacillus velezensis LG37: Transcriptome profiling and functional verification of GlnK and NmtA in ammonia (NH4+) assimilation

Bacillus velezensis LG37: Transcriptome profiling and functional verification of GlnK and NmtA in ammonia (NH4+) assimilation

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