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Hita, Oscar; Gallego, Piedad; Villalobos, Nieves; Lanas, Itziar; Blázquez, Antonio; Martín, Juan José Molina; Fernández, Javier Arístegui; Martı́n, Luisa; Guerra, Hilario

doi:10.1023/a:1021297902139

Cited by 15 publications

(1 citation statement)

References 20 publications

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“…There are several studies reporting in vitro somatic embryogenesis protocols for different plant species and also reporting that their performance can be influenced by different factors, including genotype (Moon et al 2013;Jiao et al 2018), type and maturity of explant tissue source (Moon et al 2013), choice of growth regulators (Filippov et al 2006;Verma et al 2018), carbon source (Gaj 2004;Moon et al 2013), nitrogen source (Hita et al 2003;Leljak-Levanic et al 2004), polyamines (Jiao et al 2018), basal medium strength (Prakash and Gurumurthi 2010), gelling agent, density of the culture (Moon et al 2013), subculture frequency (George et al 2008) and culture room lighting (Vidican and Cachita-Cosma 2010).…”

Section: Introductionmentioning

confidence: 99%

Addition of ionophore A23187 increases the efficiency of Cocos nucifera somatic embryogenesis

et al. 2018

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The present study reports the effect of treatment of coconut embryogenic structure explants (derived from embryogenic callus) with the calcium ionophore A23187 (0, 1, 5, 10 µM) to promote somatic embryogenesis under in vitro conditions. The results showed no significant increase in the percentage of explants forming embryogenic callus, but with 1 µM ionophore there were significant increases in the formation of embryogenic structures per callus (2.8-fold), of somatic embryos per callus (1.5-fold) and also a greater absolute number (1.5-fold) of developing plantlets per callus. The ionophore treatment also promoted a change of pattern of the expression of the gene during embryogenic callus formation. It is proposed that if the use of ionophore A23187 treatment is coupled with an embryogenic callus multiplication process there could be a potentially greater increase in the efficiency of the formation of somatic embryos and plantlets of coconut.

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Section: Introductionmentioning

confidence: 99%

Addition of ionophore A23187 increases the efficiency of Cocos nucifera somatic embryogenesis

et al. 2018

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ISSR marker‐based detection of genomic stability in Cassia occidentalis L. plantlets derived from somatic embryogenesis

Naz

Anis

Alatar

2015

Engineering in Life Sciences

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An efficient in vitro technology has been designed for mass multiplication of Cassia occidentalis (coffee senna) through somatic embryogenesis. Genetic stability of both regenerants and mother plant was evaluated. Embryogenic calli were produced on Murashige and Skoog (MS) medium supplemented with 20.0 μM 2,4‐dichlorophenoxy acetic acid (2,4‐D). Induction of somatic embryos occurred after transfer of calli to medium with reduced concentration of 2,4‐D (10.0 μM) fortified with 1.0 μM abscisic acid (ABA). Subculturing of these embryos onto the maturation medium (1.5 μM 6‐benzyladenine + 1.0 μM ABA + 0.3 μM α‐naphthalene acetic acid) favored progression of the embryos through torpedo, heart‐shaped, and cotyledonary stages; one‐half MS medium was considered the best for conversion of cotyledonary stage embryos to young plantlets. The plantlets were acclimatized to autoclaved soil rite, after which they were transferred to the green house. Among the survived plantlets, 10 plants for each primer were randomly selected for inter‐simple sequence repeat (ISSR) analysis. Of the 10 primers tested, 5 produced reproducible and monomorphic bands, 2 led to minor variation with the appearance of unique bands, and the remaining 3 did not show any banding pattern. The majority of the regenerants had similar characteristics to the donor plant, suggesting genetic homogeneity of in vitro raised plants.

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Effects of Growth Regulators, Organic Nitrogen Sources, Various Nutrient, and Non-Nutrient Adjuvants on Somatic Embryogenesis in Red Sandalwood (Pterocarpus Santalinus)

Chakraborty,

Chaitanya,

Akhtar

2023

J Plant Growth Regul

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Cited by 15 publications

References 20 publications

Addition of ionophore A23187 increases the efficiency of Cocos nucifera somatic embryogenesis

Addition of ionophore A23187 increases the efficiency of Cocos nucifera somatic embryogenesis

ISSR marker‐based detection of genomic stability in Cassia occidentalis L. plantlets derived from somatic embryogenesis

Effects of Growth Regulators, Organic Nitrogen Sources, Various Nutrient, and Non-Nutrient Adjuvants on Somatic Embryogenesis in Red Sandalwood (Pterocarpus Santalinus)

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