Addition of ionophore A23187 increases the efficiency of Cocos nucifera somatic embryogenesis

Rivera-Solís, Gustavo; Sáenz, Luis; Narváez, María; Rodríguez, Guillermo; Oropeza, Carlos

doi:10.1007/s13205-018-1392-y

Cited by 4 publications

(5 citation statements)

References 42 publications

(60 reference statements)

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“…It was reported to produce 2.8-fold embryogenic structures per callus, 1.5-fold somatic embryos per callus and 1.5-fold developing plantlets per callus. Further, it was observed that the greater expression of CnCERK (Coconut Somatic Embryogenesis Receptor Kinase) gene at initial stages of the culture period under the ionophore treatment (Rivera-Solis et al, 2018). In the present study, significant amount of embryogenic structures were obtained with calcium ionophore treatments when 2,4-D was used as the growth regulator.…”

Section: Discussionmentioning

confidence: 46%

“…Medium III contained 1 µM 2,4-D and 1 µM 22(S),23(S)-homobrassinolide. For T1, T3, T5 and T7 cultured translucent structures were treated with 10 µL of 1µM calcium ionophore A23187 deposited with a micropipette on the explant (Rivera-Solis et al, 2018).…”

Section: M E D I a P Re P A R A T I O N A N D C U L T U Re Conditionsmentioning

confidence: 99%

“…Y media formulation (Eeuwens, 1976) 3 was used as the basal medium supplemented with 5% sucrose, 0.3% phytagel and 0.25% activated charcoal (Rivera-Solis et al, 2018). Medium pH was adjusted to 5.75 before autoclaving for 20 min 0 at 121 C. All the cultures were incubated in dark 0 at 27± 2 C.…”

Section: M E D I a P Re P A R A T I O N A N D C U L T U Re Conditionsmentioning

confidence: 99%

“…It was used to substitute 2,4-D when inducing callus in some species such as wheat (Triticum aestivum) and maize (Zea mays), and sometimes considered as superior to 2,4-D (Carvalho et al, 1997). increased in the formation of embryogenic structures per callus, somatic embryos per callus and developing plantlets per callus (Rivera-Solis et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Coconut Somatic Embryogenesis of Unfertilized Ovary Derived Callus through Improved Media Composition

Jayarathna,

Balasooriya,

Vidhanaarachchi

et al. 2023

COCOS

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Coconut is an economically demanding plantation crop in Sri Lanka, having an out-breeding nature with no vegetative propagation methods. Propagation by seeds generates heterogenous population and it is not sufficient to fulfill the increasing demand for coconut plantlets. Therefore, in vitro propagation is the ultimate approach of producing clones of coconut from the mother palms of interest. Coconut in vitro propagation is achieved through somatic embryogenesis and improvements of this process will easily cater the demand for plantlets. Hence, the main objective of the study was to enhance somatic embryogenic pathway of coconut using two different auxins (2,4-Dichlorophenoxyacetic acid [2,4-D] and 3,6-Dichloro-2-methoxybenzoic acid [Dicamba]) at the concentration of 300 μM. These auxins were tested alone and in combination with calcium ionophore (A23187) and 22(S),23(S)-homobrassinolide. Translucent structures or the ear-like structures obtained from culturing of unfertilized ovaries were used for the treatments. After six weeks of the culture establishment number of embryogenic structures per one translucent structure were counted. Each treatment produced embryogenic structures but a significantly greater number of embryogenic structures were developed with 2,4-D containing media than dicamba. The results revealed the potential of using 300 μM 2,4-D with calcium ionophore (A23187) and 22(S), 23(S)-homobrassinolide to improve the somatic embryogenic pathway of unfertilized ovary derived callus of coconut.

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Section: Discussionmentioning

confidence: 46%

Section: M E D I a P Re P A R A T I O N A N D C U L T U Re Conditionsmentioning

confidence: 99%

Section: M E D I a P Re P A R A T I O N A N D C U L T U Re Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enhanced Coconut Somatic Embryogenesis of Unfertilized Ovary Derived Callus through Improved Media Composition

Jayarathna,

Balasooriya,

Vidhanaarachchi

et al. 2023

COCOS

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“…Gibberellic acid increased the expression of CnKNOX1 and decreased the expression of CnKNOX2 [74]. Treatment of coconut somatic embryogenic calli with A23187 ionophore promoted the rate of SE by increasing the somatic embryo formation and plantlets, and by altering the expression of the SERK gene [101]. Modulating the expression of these genes using exogenous chemicals may be very useful to enhance the development of coconut SE.…”

Section: Molecular Control Of Somatic Embryogenesis In Coconutmentioning

confidence: 99%

Cloning Coconut via Somatic Embryogenesis: A Review of the Current Status and Future Prospects

Kalaipandian

Kong

et al. 2021

Plants

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Coconut [Cocos nucifera L.] is often called “the tree of life” because of its many uses in the food, beverage, medicinal, and cosmetic industries. Currently, more than 50% of the palms grown throughout the world are senile and need to be replanted immediately to ensure production levels meet the present and increasing demand for coconut products. Mass replanting will not be possible using traditional propagation methods from seed. Recent studies have indicated that in vitro cloning via somatic embryogenesis is the most promising alternative for the large-scale production of new coconut palms. This paper provides a review on the status and prospects for the application of somatic embryogenesis to mass clonal propagation of coconut.

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Calcium dynamics and modulation in carrot somatic embryogenesis

et al. 2023

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Free calcium (Ca2+) is a pivotal player in different in vivo and in vitro morphogenic processes. In the induction of somatic embryogenesis, its role has been demonstrated in different species. In carrot, however, this role has been more controversial. In this work, we developed carrot lines expressing cameleon Ca2+ sensors. With them, Ca2+ levels and distribution in the different embryogenic structures formed during the induction and development of somatic embryos were analyzed by FRET. We also used different chemicals to modulate intracellular Ca2+ levels (CaCl2, ionophore A23187, EGTA), to inhibit calmodulin (W-7) and to inhibit callose synthesis (2-deoxy-D-glucose) at different times, principally during the first stages of embryo induction. Our results showed that high Ca2+ levels and the development of a callose layer are markers of cells induced to embryogenesis, which are the precursors of somatic embryos. Disorganized calli and embryogenic masses have different Ca2+ patterns associated to their embryogenic competence, with higher levels in embryogenic cells than in callus cells. The efficiency of somatic embryogenesis in carrot can be effectively modulated by allowing, within a range, more Ca2+ to enter the cell to act as a second messenger to trigger embryogenesis induction. Once induced, Ca2+-calmodulin signaling seems related with the transcriptional remodeling needed for embryo progression, and alterations of Ca2+ or calmodulin levels negatively affect the efficiency of the process.

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Addition of ionophore A23187 increases the efficiency of Cocos nucifera somatic embryogenesis

Cited by 4 publications

References 42 publications

Enhanced Coconut Somatic Embryogenesis of Unfertilized Ovary Derived Callus through Improved Media Composition

Enhanced Coconut Somatic Embryogenesis of Unfertilized Ovary Derived Callus through Improved Media Composition

Cloning Coconut via Somatic Embryogenesis: A Review of the Current Status and Future Prospects

Calcium dynamics and modulation in carrot somatic embryogenesis

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