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Abstract

Studies to determine possible differences in the pheromone communication system of three different populations of the corn stalk borer Sesamia nonagrioides (Lef.) in France. Spain. and Greece were carried out. The two main pheromone components (Z)-11-hexadecenyl acetate (Z11-16:Ac) and (Z)-11-hexadecenol (Z11-16:OH), were detected in all analyses with very small differences in the three populations. Among the minor components, analyzed by GC-MS on concentrated gland extracts from French and Greek origin females, (Z)-l1-hexadecenal (Z11-16:Ald) was detected in minor amounts. Wind-tunnel and field studies revealed similar a male response in the three populations to pheromone glands extracts and synthetic pheromone regardless of the female/male origin. The results do not support the assumption of the existence of different pheromone types of the corn stalk borer S. nonagrioides due to geographic isolation.

Production of Volatile Moth Sex Pheromones in Transgenic Nicotiana benthamiana Plants

“…Species-specificity in these cases is provided by the precise ratio in the blend. For instance, Krokos et al [ 30 ] tested the response of Sesamia nonagrioides (Lefèbvre) males to different blends of pheromone compounds, identifying a 90 : 10 : 5 blend of Z11-16OAc:Z11-16OH:Z11-16Ald as the most effective. This feature makes the genetic design of plant emitters for attract-and-kill strategies in moths extremely challenging, because ensuring the right proportions of the three compounds requires a tight control of several factors, from gene expression to enzymatic activity and differential release ratios.…”

Disposable Polydimethylsiloxane (PDMS)-Coated Fused Silica Optical Fibers for Sampling Pheromones of Moths

“…These so-called “direct-contact SPME” samples were qualitatively and quantitatively similar to solvent extractions. This method has since been used successfully in a number of studies using various types of coating: 100 μm PDMS- [ 21 – 24 ], 7 μm PDMS- [ 20 , 25 ], 65 μm CW/DVB- [ 26 – 33 ], and 50/30 μm DVB/CAR/PDMS-coated fibers [ 34 ]. Insect cuticular hydrocarbons have been sampled successfully this way as well [ 16 ].…”

Recent trends in management strategies for two major maize borers: Ostrinia nubilalis and Sesamia nonagrioides

“…Since then, they became a model system for basic and applied studies on pheromone biosynthesis, chemoreception, evolution, and genetics (Mas et al 2000;de Santis et al 2006;Lassance 2010Lassance , 2016Unbehend et al 2021). Females of S. nonagrioides release a four-component mixture of Z-11-hexadecenyl acetate (Z11-16:Ac), Z-11-hexadecenol (Z11-16:OH), Z-11-hexadecenal (Z11:14:Al), and dodecyl acetate (12:Ac) (Sreng et al 1985;Mazomenos 1989;Krokos et al 2002), while O. nubilalis female emits a binary blend of (Z)-11-tetradecenyl acetate (Z11-14:Ac) and (E)-11-tetradecenyl acetate (E11-14:Ac) (Klun et al 1967;Russell et al 1975). Their pheromone blends are different enough so there is no pheromonal crossattraction among the two species (Gemeno et al 2006;Cruz and Eizaguirre 2016).…”

“…Both species exhibit variability in the proportion of the pheromone components across populations, making this tactic difficult to implement without prior knowledge on the pheromone composition of the target population. In the case of S. nonagrioides, despite variability in the pheromone blend composition emitted by females from different geographic populations (Spain, France, and Greece), males are attracted to a broad range of pheromone blends (Krokos et al 2002). However, slight changes in the ratio of the components of the synthetic pheromone blend for monitoring S. nonagrioides can enhance efficacy and selectivity in male traps in a given location (Mazomenos 1989;Sans et al 1997;Krokos et al 2002).…”