2021
DOI: 10.34133/2021/9891082
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Production of Volatile Moth Sex Pheromones in Transgenic Nicotiana benthamiana Plants

Abstract: Plant-based bioproduction of insect sex pheromones has been proposed as an innovative strategy to increase the sustainability of pest control in agriculture. Here, we describe the engineering of transgenic plants producing (Z)-11-hexadecenol (Z11-16OH) and (Z)-11-hexadecenyl acetate (Z11-16OAc), two main volatile components in many Lepidoptera sex pheromone blends. We assembled multigene DNA constructs encoding the pheromone biosynthetic pathway and stably transformed them into Nicotiana benthamiana plants. Th… Show more

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Cited by 20 publications
(54 citation statements)
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“…However, while the pheromones of some species can be cheaply manufactured by synthetic chemistry, the pheromones of many insect species have complex structures requiring stereoselective steps making them difficult and expensive to produce (Petkevicius et al, 2020). To provide proof-of-concepts for the biological synthesis of pheromone components, several studies have successfully produced Lepidopteran sex pheromones in heterologous systems including yeast (Hagström et al, 2013;Holkenbrink et al, 2020;Konrad et al, 2017;Petkevicius et al, 2020) and N. benthamiana (Ding et al, 2014;Mateos-Fernández et al, 2021;Xia et al, 2022Xia et al, , 2020. In the latter, both transient agroinfiltration and stable transgenic approaches have been trialed, though retardation of growth was observed in the best-producing transgenic lines (Mateos-Fernández et al, 2021).…”
Section: Discussionmentioning
confidence: 99%
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“…However, while the pheromones of some species can be cheaply manufactured by synthetic chemistry, the pheromones of many insect species have complex structures requiring stereoselective steps making them difficult and expensive to produce (Petkevicius et al, 2020). To provide proof-of-concepts for the biological synthesis of pheromone components, several studies have successfully produced Lepidopteran sex pheromones in heterologous systems including yeast (Hagström et al, 2013;Holkenbrink et al, 2020;Konrad et al, 2017;Petkevicius et al, 2020) and N. benthamiana (Ding et al, 2014;Mateos-Fernández et al, 2021;Xia et al, 2022Xia et al, , 2020. In the latter, both transient agroinfiltration and stable transgenic approaches have been trialed, though retardation of growth was observed in the best-producing transgenic lines (Mateos-Fernández et al, 2021).…”
Section: Discussionmentioning
confidence: 99%
“…The genes encoding moth acetyltransferases involved in pheromone biosynthesis have not yet been identified and previous studies have employed either a plant enzyme, EaDAcT (Ding et al, 2014;Mateos-Fernández et al, 2021) or the yeast enzyme ScATF1 (Ding et al, 2016;Xia et al, 2022), with the latter producing more acetate. When comparing the activities of these enzymes we observed differences in the levels of product obtained from two yeast enzymes, ScATF1 and SpATf1-2, which differ at only one amino acid (Figure 1) Inducible gene expression systems are essential tools in metabolic engineering.…”
Section: Discussionmentioning
confidence: 99%
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“…However, several studies have reported the accumulation of unintended side products, presumably produced by the off-target activities of endogenous N. benthamiana enzymes such as oxidases and glycosylases [8][9][10][11][12][13][14][15][16][17][18] . Although the activity of endogenous enzymes has been exploited in some studies to produce novel molecules 8 or to compensate for the lack of a known enzyme 19 , derivatization of molecules for the most part is a disadvantage, reducing the potential purity and yield of the target compound.…”
Section: Introductionmentioning
confidence: 99%