Number of spikelets per panicle is one of the important characters for rice breeding programs to increase yield potential. The generation mean analysis based on six populations: viz., P1, P2, F 1 , F 2 , BC1, and BC2 of IR80151A/CH1 with utilizing cytoplasmic-genetic male, was performed. The results showed that dominant gene action was more important than additive type implying that selection in early segregating generations is ineffective and the character could be better exploited through heterosis breeding. Since hybrid rice was used the advantage of the dominant effect through heterosis, due to the role of more favorable dominant genes in F 1 hybrid than in both parents. In addition, utilizing cytoplasmic-genetic male sterility in the populations could specify gene control of fertility restoration in hybrid rice. The results revealed that two dominant complementary genes with epistasis controlled the fertility-restoration of wild-abortive cytoplasmic male sterility system (WA-CMS). This information will be useful for future rice breeding in three-line hybrid system.