Fine mapping of Rf3 and Rf4 fertility restorer loci of WA-CMS of rice (Oryza sativa L.) and validation of the developed marker system for identification of restorer lines

Suresh, P. Balaji; Srikanth, B.; Kishore, Vimal; Rao, I. Subhakara; Vemireddy, Lakshminarayana R.; Dharika, N.; Sundaram, R. M.; Ramesha, M. S.; Rao, K. R. S. Sambasiva; Viraktamath, B. C.; Neeraja, C. N.

doi:10.1007/s10681-012-0737-6

Cited by 66 publications

(33 citation statements)

References 29 publications

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“…For fertility restoration, markers linked to Rf3 and Rf4 were used. Three SSR markers, comprising one RM marker - RM 6100 (160 bp in non-restorer line and 175 bp for restorer line), two candidate gene markers DRCG- RF4 -14 (885 bp in non-restorer line and 845 bp for restorer line) and DRCG- RF4 -8(1096 bp in non-restorer line and 760 bp for restorer line) for Rf4 locus and two SSR markers DRRM-RF3-5 (140 bp in non-restorer line and 160 bp for restorer line) and DRRM-RF3-10 (140 bp in non-restorer line and 150 bp for restorer line) for Rf3 reported by Suresh et al (2012) were used in BC 2 F 2 and BC 2 F 3 generations. The details of markers sequences were given in Table 1.…”

Section: Methodsmentioning

confidence: 83%

Functional Marker Assisted Improvement of Stable Cytoplasmic Male Sterile Lines of Rice for Bacterial Blight Resistance

et al. 2017

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Bacterial blight (BB), caused by Xanthomonas oryzae pv.oryzae is one among the major diseases in rice, which in severe condition cause losses up to 60% in total yield. Marker assisted pyramiding of three broad spectrum BB resistance genes (xa5, xa13, and Xa21) in prominent rice varieties is the most economical and effective strategy for the management of the BB disease. We report here the pyramiding of three genes (xa5, xa13, and Xa21) in maintainer lines (CO 2B, CO 23B, and CO 24B) of three promising wild abortive cytoplasmic male sterile lines (CO 2A, CO 23A, and CO 24A) through functional markers assisted back cross breeding. IRBB60 with xa5, xa13, and Xa21 genes is used as a donor parent. BC2F1 and BC2F2 generations from a cross of CO 2B, CO 23B, and CO 24B with IRBB60 were evaluated for bacterial blight and non-fertility restoration. In BC2F1, plants with all three resistance genes (xa5, xa13, and Xa21) and high parent genome recovery was identified. In BC2F2, plants with all resistance genes and without fertility restorer (Rf3 and Rf4) were selected. Based on agronomic traits, BB resistance and maintenance of sterility, two plants each in CO 2B × IRBB60, CO 24B × IRBB60 and one plant in CO 23B × IRBB60 combinations were identified. The identified lines were crossed with respective male sterile lines for conversion of improved B line into CMS line through back-crossing, in addition to selfing. The plants with high recurrent genome and phenotypically similar to parental lines and sterile are being used for the hybrid rice development program. Currently, using these lines (improved CMS line), test crosses were made to develop new rice hybrids. Hybrids combinations viz., CO 23A × AD08009R and CO 24A × IET20898R were found to be stable at different locations with high yield. The R line used in this study has been introgressed with xa5, xa13, and Xa21 genes in a separate breeding program. These new hybrids with resistance against bacterial blight will increase the crop production at BB environment.

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Section: Methodsmentioning

confidence: 83%

Functional Marker Assisted Improvement of Stable Cytoplasmic Male Sterile Lines of Rice for Bacterial Blight Resistance

et al. 2017

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“…We also found a sequence variant of orf352 in an RT102 CMS line derived from O. rufipogon (Okazaki et al 2013). However, the Rf genes in nuclear genome have not yet been cloned, although two major Rf genes, Rf3 and Rf4, have been mapped on the chromosomes 1 and 10, respectively (Lu et al [1997]; Yao et al [1997]; Tan et al [2008]; Jing et al [2001]; Ahmadikhah and Karlov [2006]; Ngangkham et al [2010]; Suresh et al [2012]). In this study, we report the cloning of Rf4 .…”

Section: Resultsmentioning

confidence: 99%

“…The pollen fertility was not completely restored by the introduced Rf4 gene. Other fertility restorer genes, such as the Rf3 gene on chromosome 1, might be necessary for fully restoration of WA-CMS (Suresh et al [2012]).…”

Section: Resultsmentioning

confidence: 99%

A fertility restorer gene, Rf4, widely used for hybrid rice breeding encodes a pentatricopeptide repeat protein

Kazama

Toriyama

2014

Rice

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BackgroundUncontrolled expression of a certain mitochondrial gene often causes cytoplasmic male sterility (CMS) in plants. This phenotype is prevented by the presence of a fertility restorer (Rf) gene in the nuclear genome. Such CMS/Rf systems have been successfully used for breedings of F1 hybrid cultivars. In rice, approximately 99% of F1 hybrid cultivars have been developed using a wild abortive type of CMS (WA-CMS) and its Rf genes. Recently, a newly identified mitochondrial gene, orf352, was reported as a WA-CMS-causing gene.FindingsWe cloned and functionally characterized Rf4, a major Rf gene for WA-CMS. We revealed that Rf4 encoded a pentatricopeptide repeat-containing protein and reduced the orf352-containing transcripts, thereby restoring pollen fertility.ConclusionsThrough a map-based cloning, we have independently identified an allele of a recently reported Rf4 gene and demonstrated that the fertility restoration is controlled sporophytically.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-014-0028-z) contains supplementary material, which is available to authorized users.

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“…Unlike the two-line hybrid rice, restorer lines for three-line hybrid rice have to carry fertility restorer ( Rf ) genes that are genetically specific to some CMS type and different from each other (Li et al, 2005, 2007), which greatly limit the exploitation of heterosis because of the specific restoring-maintaining relationship. Today, there are about six Rf genes having been identified in rice, of which Rf3 and Rf4 for WA-CMS (Suresh et al, 2012; Luo et al, 2013; Tang et al, 2014), Rf5 and Rf6 for HL-CMS (Hu et al, 2012b; Huang et al, 2015) have been cloned or finely mapped, and can be well used for molecular breeding. This will provide us the possibility to breed wide-spectrum restorer lines that can be used not only for two-line hybrid rice, but also for HL- and WA-type hybrid rice by pyramiding these four Rf genes together.…”

Section: Introductionmentioning

confidence: 99%

Development of Elite BPH-Resistant Wide-Spectrum Restorer Lines for Three and Two Line Hybrid Rice

Fan

Chen

et al. 2017

Front. Plant Sci.

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Hybrid rice has contributed significantly to the world food security. Breeding of elite high-yield, strong-resistant broad-spectrum restorer line is an important strategy for hybrid rice in commercial breeding programs. Here, we developed three elite brown planthopper (BPH)-resistant wide-spectrum restorer lines by pyramiding big-panicle gene Gn8.1, BPH-resistant genes Bph6 and Bph9, fertility restorer genes Rf3, Rf4, Rf5, and Rf6 through molecular marker assisted selection. Resistance analysis revealed that the newly developed restorer lines showed stronger BPH-resistance than any of the single-gene donor parent Luoyang-6 and Luoyang-9. Moreover, the three new restorer lines had broad spectrum recovery capabilities for Honglian CMS, Wild abortive CMS and two-line GMS sterile lines, and higher grain yields than that of the recurrent parent 9,311 under nature field conditions. Importantly, the hybrid crosses also showed good performance for grain yield and BPH-resistance. Thus, the development of elite BPH-resistant wide-spectrum restorer lines has a promising future for breeding of broad spectrum BPH-resistant high-yield varieties.

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Fine mapping of Rf3 and Rf4 fertility restorer loci of WA-CMS of rice (Oryza sativa L.) and validation of the developed marker system for identification of restorer lines

Cited by 66 publications

References 29 publications

Functional Marker Assisted Improvement of Stable Cytoplasmic Male Sterile Lines of Rice for Bacterial Blight Resistance

Functional Marker Assisted Improvement of Stable Cytoplasmic Male Sterile Lines of Rice for Bacterial Blight Resistance

A fertility restorer gene, Rf4, widely used for hybrid rice breeding encodes a pentatricopeptide repeat protein

Development of Elite BPH-Resistant Wide-Spectrum Restorer Lines for Three and Two Line Hybrid Rice

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