A Study on Comparative Fertility Restoration in A2 and A4 Cytoplasms and Its Implication in Breeding Hybrid Pigeonpea [Cajanus cajan (L.) Millspaugh]

Abstract

Abstract

Transcriptome profiling of differentially expressed genes in cytoplasmic male-sterile line and its fertility restorer line in pigeon pea (Cajanus cajan L.)

“…Therefore, hybrid seed technology based on cytoplasmic male-sterility has given a chance to overcome the production constraints by efficiently increasing the yield potential in pigeon pea [45]. However, among the eight systems, A 2 and A 4 CMS system obtained from the wild-type Cajanus scarabaeoides and Cajanus cajanifolius respectively, are regarded as highly potential CMS systems [46][47][48]. The gene associated with CMS in A 4 cytoplasm pigeon pea has been reported [49].…”

Genetics of fertility restoration in A2‐based cytoplasmic genetic male sterility system in pigeonpea (Cajanus cajan)

“…However, the inheritance of fertility restoration is independent of the source of male sterility system. The gene action is unique to every individual CGMS system and was reported by Lay and Saxena (2011), Sawargaonkar et al (2012), Choudhary and Singh (2015), Saroj et al (2015), Sultana and Saxena (2017) and Saxena et al (2018) in pigeonpea. On comparison of the three methods utilized to assess the fertile and sterile plants, all the three methods showed same results for inheritance of fertility restoration.…”

“…Sharma et al (2019) studied the inheritance of fertility restoration in A 2 CGMS system based on pollen fertility and reported monogenic dominant gene action for fertility restoration. The segregation ratio of 3:1 for fertility restoration was observed in A 4 CGMS system of pigeonpea showing single dominant gene involved in the inheritance (Choudhary & Singh, 2015; Dalvi et al, 2008).…”

“…(a) Plants with uniform sized, stained, large pollen grain and pollen fertility above 80% (Type A); (b) plants with stained and mixture of large and small sized pollen grains as in the F 1 hybrids and pollen fertility above 80% (Type B); and (c) plants with unstained shrivelled pollen grains and 100% pollen sterility (Type C) [Color figure can be viewed at wileyonlinelibrary.com] confirmed that fertility restoration is controlled by single dominant gene.The conclusive evidence from the assessment of segregating population involving the crosses, namely, CORG 990047A Â AK 261322, CORG 990052A Â AK 261322 and CORG 7A Â AK 261322, based on anther colour, pollen grain fertility and pollen grain morphology and staining proved that the inheritance of fertility restoration was controlled by single dominant gene. However, the inheritance of fertility restoration is independent of the source of male sterility system.The gene action is unique to every individual CGMS system and was reported byLay and Saxena (2011), Sawargaonkar et al (2012),Choudhary and Singh (2015),Saroj et al (2015),Sultana and Saxena (2017) andSaxena et al (2018) in pigeonpea. On comparison of the three methods utilized to assess the fertile and sterile plants, all the three methods showed same results for inheritance of fertility restoration.…”

De novo Assembly and Characterization of Cajanus scarabaeoides (L.) Thouars Transcriptome by Paired-End Sequencing

“…The phenomenon of heterosis has been effectively used for genetic improvement in pigeonpea as it shows considerable extent of natural outcrossing. A number of cytoplasmic male sterile (CMS) systems are available in pigeonpea but CMS lines derived from C. scarabaeoides (A2 cytoplasm) has been widely utilized to develop promising commercial hybrids (Choudhary and Singh, 2015 ). Unfortunately, very narrow genomic information is available for C. scarabaeoides .…”