89Zr as a promising radionuclide and it’s applications for effective cancer imaging

Sarcan, Elif Tugce; Silindir-Gunay, Mine; Özer, A. Yekta; Hartman, Neil R.

doi:10.1007/s10967-021-07928-0

Cited by 8 publications

(3 citation statements)

References 107 publications

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“…Most importantly, the radiotracer should have a long-lived physical half-life (Mohammadpour-Ghazi et al 2023 ). Zirconium-89 (t ½ = 78 h), a positron emitting radionuclide of relatively low energy, is produced via the proton bombardment of naturally abundant yttrium-89 using a biomedical cyclotron (Sarcan et al 2021 ; Mohammadpour-Ghazi et al 2023 ; Gordon and Vivian 1984 ) and its production is characterized by high purity and high specific activity (Sarcan et al 2021 ; Holland et al 2009 ; Ikotun and Lapi 2011 ). In the current study, we described a method for efficient and rapid labelling of leukocytes with a PET-emitting, long-lived radioisotope, i.e., 89 Zr.…”

Section: Discussionmentioning

confidence: 99%

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89Zr-leukocyte labelling for cell trafficking: in vitro and preclinical investigations

Kahts,

Guo,

Kommidi

et al. 2023

EJNMMI radiopharm. chem.

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Background The non-invasive imaging of leukocyte trafficking to assess inflammatory areas and monitor immunotherapy is currently generating great interest. There is a need to develop more robust cell labelling and imaging approaches to track living cells. Positron emission tomography (PET), a highly sensitive molecular imaging technique, allows precise signals to be produced from radiolabelled moieties. Here, we developed a novel leukocyte labelling approach with the PET radioisotope zirconium-89 (89Zr, half-life of 78.4 h). Experiments were carried out using human leukocytes, freshly isolated from whole human blood. Results The 89Zr-leukocyte labelling efficiency ranged from 46 to 87% after 30–60 min. Radioactivity concentrations of labelled cells were up to 0.28 MBq/1 million cells. Systemically administered 89Zr-labelled leukocytes produced high-contrast murine PET images at 1 h–5 days post injection. Murine biodistribution data showed that cells primarily distributed to the lung, liver, and spleen at 1 h post injection, and are then gradually trafficked to liver and spleen over 5 days. Histological analysis demonstrated that exogenously 89Zr-labelled human leukocytes were present in the lung, liver, and spleen at 1 h post injection. However, intravenously injected free [89Zr]Zr4+ ion showed retention only in the bone with no radioactivity in the lung at 5 days post injection, which implied good stability of radiolabelled leukocytes in vivo. Conclusions Our study presents a stable and generic radiolabelling technique to track leukocytes with PET imaging and shows great potential for further applications in inflammatory cell and other types of cell trafficking studies.

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Section: Discussionmentioning

confidence: 99%

“…89 Zr can be obtained with high radionuclide purity and at high yields. 89 Zr benefits from its relatively low mean positron emission energy of 395.5 keV, which translates into high image resolution (Fairclough et al 2016 ; Deri et al 2013 ; Sarcan et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

89Zr-leukocyte labelling for cell trafficking: in vitro and preclinical investigations

Kahts,

Guo,

Kommidi

et al. 2023

EJNMMI radiopharm. chem.

View full text Add to dashboard Cite

show abstract

“…These radiopharmaceuticals provide imaging within 24 hours of administration. In this context, 89 Zr is currently being studied for PET [2][3][4][5][6][7][8][9][10][11][12] applications and has received considerable attention in radioimmunotherapy applications because of its favourable decay characteristics and half-life period (t 1/2 = 78.4 h) making it useful for labelling monoclonal antibodies. 13 To deliver 89 Zr to a given target, a powerful chelator must be bound to the tetravalent metal ion to prevent the release of the radionuclide into the human body.…”

Section: Introductionmentioning

confidence: 99%