8-OHdG and hOGG1 as Oxidative DNA Damage Markers in Acne Vulgaris Patients Under Isotretinoin Treatment

Abstract

Background: Acne vulgaris is the most common inflammatory skin disease. It is primarily observed in adolescents and is characterized by comedones, papules, pustules, nodules, and cysts on the face, back, chest, chin, and body skin. Acne vulgaris affects about 80% of teenagers and continues beyond the age of 25 years in 3% of men and 12% of women in the world. Isotretinoin is one of the most common treatment agents for acne vulgaris, which causes oxidative DNA damage in the cell. As an important indicator of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine is repaired with an enzyme called human 8-oxoguanine DNA glycosylase 1 (hOGG1). Objectives: We aimed to evaluate oxidative DNA damage in acne vulgaris before and after isotretinoin treatment by measuring the 8-OHdG and hOGG1 levels. Methods: The 8-OHdG and hOGG1 levels were evaluated from serum samples using the enzyme-linked immunosorbent assay (ELISA) method. Both the serum 8-OHdG (P < 0.05; P < 0.0001) and hOGG1 (P < 0.05; P = 0.04) levels were found to be statistically higher in the sixth month after isotretinoin treatment. Results: In this first report, the 8-OHdG and hOGG1 levels were found to be statistically significantly high after isotretinoin treatment. According to our results, the 8-OHdG level increased under isotretinoin administration in acne vulgaris patients. Conclusions: Consequently, healing via hOGG1 likely continues after dropping isotretinoin for DNA.

Assessment of 8-Hydroxy-2-Deoxyguanosine in Vitiligo Patients

“…8-OHdG levels in acne vulgaris were measured by Izmirli et al (20). The 8-OHdG level was found to be greater in patients (21.0 4.3 ng/mL) compared to the controls (10.07 4.01 ng/mL) in the research described.…”

Markers of Oxidative Stress in Patients with Acne: A Literature Review

“…The studies analyzed in this review support the hypothesis of the occurrence of OS following isotretinoin therapy, a hypothesis that was stated almost two decades ago. Izmirli et al showed that the serum levels of 8-OHdG and hOGG1, a biomarker for 8-OHdG repair, increased significantly after the treatment with isotretinoin and suggested that the serum levels of hOGG1 increased in response to DNA damage but was not sufficient for DNA repair [71]. However, Alaqrawi et al did not find statistically significantly differences between the level of 8-OHdG before and after the treatment with isotretinoin [69].…”