8-Nitro-cGMP is a promoter of osteoclast differentiation induced by RANKL

Kaneko, Kotaro; Miyamoto, Yukio; Tsukuura, Risa; Sasa, Kiyohito; Akaike, Takaaki; Fujii, Shigemoto; Yoshimura, Kenichi; Nagayama, Kazuhiro; Hoshino, Mitsunori; Inoue, Satoshi; Maki, Koutaro; Baba, Kazuyoshi; Chikazu, Daichi; Kamijo, Ryutaro

doi:10.1016/j.niox.2017.11.006

Cited by 11 publications

(10 citation statements)

References 31 publications

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“…Confocal microscopy revealed that although B. abortus S19 was located both extracellularly and intracellularly, large numbers of bacteria were observed inside mature osteoclasts at different cellular depths. Osteoclasts are multinucleated boneresorbing cells that differentiate from monocyte/macrophage lineage under the effect of two osteoclastogenic cytokines that are required for their survival and differentiation: receptor activator of nuclear factor B (NF-B) ligand (RANKL) and macrophage colonystimulating factor (MCSF) (55)(56)(57)(58). Osteoclasts degrade bone matrix through secretion of several osteolytic enzymes and acids that solubilize bone components (59,60), and previous studies have reported that activated osteoclasts play a pivotal role in bone destruction, such as an inflammatory bone loss and rheumatoid arthritis (61).…”

Section: Discussionmentioning

confidence: 99%

The NOD- scid IL2rγ ^null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

et al. 2019

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Osteoarticular brucellosis is the most common complication in Brucella-infected humans regardless of age, sex, or immune status. The mechanism of bone destruction caused by Brucella species remained partially unknown due to the lack of a suitable animal model. Here, to study this complication, we explored the suitability of the use of the NOD-scid IL2rγnull mouse to study osteoarticular brucellosis and examined the potential use of this strain to evaluate the safety of live attenuated vaccine candidates. Mice were inoculated intraperitoneally with a single dose of 1 × 104, 1 × 105, or 1 × 106 CFU of B. abortus S19 or the vaccine candidate B. abortus S19ΔvjbR and monitored for the development of side effects, including osteoarticular disease, for 13 weeks. Decreased body temperature, weight loss, splenomegaly, and deformation of the tails were observed in mice inoculated with B. abortus S19 but not in those inoculated with S19ΔvjbR. Histologically, all S19-inoculated mice had a severe dose-dependent inflammatory response in multiple organs. The inflammatory response at the tail was characterized by the recruitment of large numbers of neutrophils, macrophages, and osteoclasts with marked bone destruction. These lesions histologically resembled what is typically observed in Brucella-infected patients. In contrast, mice inoculated with B. abortus S19ΔvjbR did not show significant bone changes. Immunofluorescence, in situ hybridization, and confocal imaging demonstrated the presence of Brucella at the sites of inflammation, both intra- and extracellularly, and large numbers of bacteria were observed within mature osteoclasts. These results demonstrate the potential use of the NOD-scid IL2rγnull mouse model to evaluate vaccine safety and further study osteoarticular brucellosis.

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Section: Discussionmentioning

confidence: 99%

The NOD- scid IL2rγ ^null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

et al. 2019

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“…iNOS and eNOS are expressed in human PDL stem cells (103,104). Previous studies revealed the presence of sGC and cGMP in mouse bone marrow macrophages ( 105), osteoclasts (105)(106)(107), and osteocytes (108). Davidovitch et al (109,110) performed immunohistochemistry (IHC) on alveolar bone sections obtained from cats and revealed that cGMP expression was increased in the PDL fibroblast cells stained intensely for; however, most cGMP expression was not detected through IHC staining in osteoblasts.…”

Section: Effects Of No On Orthodontic Tooth Movementmentioning

confidence: 99%

“…It has been observed that the promoting effect of iNOS on osteoclasts is mediated through the NO/cGMP pathway. Kaneko et al (105) revealed that 8-nitro-cGMP, a NO-dependent derivative of cGMP in mammals, increased RANKL mRNA expression, and enhanced osteoclast differentiation. The reduction in cGMP levels due to the inhibition of NOS caused RANKL-induced osteoclast differentiation suppression.…”

Section: Osteoclastsmentioning

confidence: 99%

Role of nitric oxide in orthodontic tooth movement (Review)

Yan

Xie

et al. 2021

Int J Mol Med

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Nitric oxide (NO) is an ubiquitous signaling molecule that mediates numerous cellular processes associated with cardiovascular, nervous and immune systems. NO also plays an essential role in bone homeostasis regulation. The present review article summarized the effects of NO on bone metabolism during orthodontic tooth movement in order to provide insight into the regulatory role of NO in orthodontic tooth movement. Orthodontic tooth movement is a process in which the periodontal tissue and alveolar bone are reconstructed due to the effect of orthodontic forces. Accumulating evidence has indicated that NO and its downstream signaling molecule, cyclic guanosine monophosphate (cGMP), mediate the mechanical signals during orthodontic-related bone remodeling, and exert complex effects on osteogenesis and osteoclastogenesis. NO has a regulatory effect on the cellular activities and functional states of osteoclasts, osteocytes and periodontal ligament fibroblasts involved in orthodontic tooth movement. Variations of NO synthase (NOS) expression levels and NO production in periodontal tissues or gingival crevicular fluid (GCF) have been found on the tension and compression sides during tooth movement in both orthodontic animal models and patients. Furthermore, NO precursor and NOS inhibitor administration increased and reduced the tooth movement in animal models, respectively. Further research is required in order to further elucidate the underlying mechanisms and the clinical application prospect of NO in orthodontic tooth movement.

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“…8-Nitro-cGMP is involved in a number of cellular phenomena including cardiac cell senescence, neuronal cell death, the antioxidant stress response, chondrocyte proliferation, bone growth, osteoclast differentiation, and stomatal closure. (10,14,32,34,(40)(41)(42)(43) 8-Nitro-cGMP can activate cGMP-dependent protein kinase and stimulate vasorelaxation, similar to the well-known NO second messenger, cGMP. (32) Moreover, 8-nitro-cGMP is active stronger than cGMP under physiological conditions because the nitrated nucleotide is resistant to phosphodiesterase-dependent degradation.…”

Section: Formation Of Electrophilic Redox Signaling Molecules and Thementioning

confidence: 99%

Regulation of redox signaling by reactive sulfur species

Kasamatsu

Ihara

2021

J. Clin. Biochem. Nutr.

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Reactive sulfur species, such as cysteine persulfide, are produced endogenously at significant levels in cells and have rapidly emerged as common biomolecules. By virtue of improved analytical methods for detecting reactive persulfides, it has been demonstrated that these reactive molecules exhibit unique chemical properties and are present in various forms in vivo. Accumulating evidence has suggested that persulfides may be involved in a variety of biological processes, such as antioxidant and anti-inflammatory responses, biosynthesis of sulfurcontaining molecules, mitochondrial energy metabolism via sulfur respiration, and cytoprotection via regulation of redox signal transduction induced by endogenous and exogenous electrophiles. Elucidation of the persulfide-dependent metabolism of redox signals is expected to facilitate our understanding of the importance of persulfides in regulating redox signals.

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8-Nitro-cGMP is a promoter of osteoclast differentiation induced by RANKL

Cited by 11 publications

References 31 publications

The NOD- scid IL2rγ ^null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

The NOD- scid IL2rγ ^null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

Role of nitric oxide in orthodontic tooth movement (Review)

Regulation of redox signaling by reactive sulfur species

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8-Nitro-cGMP is a promoter of osteoclast differentiation induced by RANKL

Cited by 11 publications

References 31 publications

The NOD- scid IL2rγ null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

The NOD- scid IL2rγ null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

Role of nitric oxide in orthodontic tooth movement (Review)

Regulation of redox signaling by reactive sulfur species

Contact Info

Product

Resources

About

The NOD- scid IL2rγ ^null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety

The NOD- scid IL2rγ ^null Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety