8-Hydroxy-2-deoxyguanosine ameliorates high-fat diet-induced insulin resistance and adipocyte dysfunction in mice

Epigallocatechin Exerts Anti‐Obesity Effect in Brown Adipose Tissue

“…Differentiating cells were subjected to 4 days of total exposure to each of the test compounds (100 μmol/L concentration). Each compound was added at concentrations of 20, 50, and 100 μmol/L to determine morphological cell differentiation following staining with Oil Red O (ORO) stain containing 10 % (v/v) formalin . After staining, the cells were washed with distilled water and representative pictures were taken under the microscope.…”

“…For cell culture and differentiation, the 3T3-L1 murine cell lines were purchased from the American Type Culture Collection (Rockville, MD, USA) and were cultured as described previously. [24] The cells were treated with catechins and GA on day 0 and day 2 to investigate their adipogenesis. Differentiating cells were subjected to 4 days of total exposure to each of the test compounds (100 μmol/L concentration).…”

“…Each compound was added at concentrations of 20, 50, and 100 μmol/L to determine morphological cell differentiation following staining with Oil Red O (ORO) stain containing 10 % (v/v) formalin. [24] After staining, the cells were washed with distilled water and representative pictures were taken under the microscope. For quantification, 100 % isopropanol was added and the absorbance was measured at 490 nm.…”

Exogenous 8‐hydroxydeoxyguanosine ameliorates liver fibrosis through the inhibition of Rac1‐NADPH oxidase signaling

“…In an oxidative stress, the reaction of the hydroxyl radical with the DNA nucleoside deoxyguanosine results in the formation of endogenous 8‐hydroxydeoxyguanosine (8‐OHdG) . However, recent several reports have been suggested that exogenous 8‐OHdG could reduce ROS production and proinflammatory mediators through the inhibition of Rac1 and NOX activation in inflammation‐based diseases …”

The effects of phenolic glycosides from Betula platyphylla var. japonica on adipocyte differentiation and mature adipocyte metabolism

“…Cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Hyclone, Logan, UT) supplemented with 10% fetal bovine serum (FBS; Hyclone) and 1% penicillin/streptomycin and incubated at 37 °C in 5% CO 2 . 3T3-L1 cells were differentiated in a standard protocol where differentiation media 1, 2, and 3 were used 23 . Briefly, two days after the 3T3-L1 preadipocytes reached 100% confluency (day 0), cells were treated with differentiation media 1 where 5 µg/mL insulin (Sigma, St. Louis, MO), 0.25 mM dexamethasone (Sigma), and 0.5 mM 1-methyl-3-isobutylxanthine (Sigma) was added to 10% FBS DMEM media.…”