Epigallocatechin Exerts Anti‐Obesity Effect in Brown Adipose Tissue

Kim, Hae-Soo; Moon, Jae‐Hak; Kim, Young‐Min; Huh, Jooyoung

doi:10.1002/cbdv.201900347

Cited by 9 publications

(8 citation statements)

References 26 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1A). These molecules included epigallocatechin, 14 ursolic acid, 15 carvacrol 16 and other natural products that have been previously shown to have anti-obesity effects, which confirms the feasibility of the screening method. In addition, we identified a compound, epigoitrin (EP), an alkaloid from Radix isatidis , whose structural formula is shown in Fig.…”

Section: Resultssupporting

confidence: 63%

Epigoitrin alleviates lipid and glucose metabolic disorders induced by a high-fat diet

Xiao

et al. 2022

Food Funct.

View full text Add to dashboard Cite

show abstract

Section: Resultssupporting

confidence: 63%

Epigoitrin alleviates lipid and glucose metabolic disorders induced by a high-fat diet

Xiao

et al. 2022

Food Funct.

View full text Add to dashboard Cite

show abstract

“…We found that 0.4 µg/mL of chia seed showed high MMP, directly representing active mitochondrial thermogenesis. In this context, epigallocatechin [28,29] and aloe-emodin [30] have been reported for their anti-obesity potential via the inhibition of adipogenesis and the enhancement of fatty acid oxidation. Figure 4 shows the results for the accumulated oil red O stain concentration in control and chia seed-treated maturing adipocytes after 14 days.…”

Section: Resultsmentioning

confidence: 99%

Beneficial Fatty Acid Ratio of Salvia hispanica L. (Chia Seed) Potentially Inhibits Adipocyte Hypertrophy, and Decreases Adipokines Expression and Inflammation in Macrophage

Subash-Babu

Almaiman

Al-Harbi

et al. 2020

Foods

View full text Add to dashboard Cite

The present study aimed to determine the role of Salvia hispanica L., (chia seed) fatty acid content in adipocyte lipid accumulation and human macrophage immunoregulatory potential. Chia seed fatty acid was extracted using hexane by the cold percolation method. A gas chromatography-mass spectrometry (GC-MS) analysis showed a 3:1 ratio of omega 3 and omega 6 fatty acid composition and it was more beneficial for human health. We treated it with increasing concentrations (0–6.4 μg/mL) of chia seed fatty acid extract to determine the cytotoxicity on the preadipocytes and macrophage; no significant cytotoxicity was observed. Chia seed, in 0.2 and 0.4 μg/mL doses, significantly arrested adipocyte hypertrophy and macrophage foam cell development. The gene expression levels of adipocyte confirmed the increased expression of adipocyte mitochondrial thermogenesis related genes, such as uncoupling protein-1 (UCP-1), peroxisome proliferator activated receptor gamma coactivator 1 alpha (PPARγC1α) and PR domain containing 16 (PRDM16); and the down regulated expression of the lipid synthesis related gene sterol regulatory element binding of protein-1c (SREBP-1c). In addition, adipogenesis related genes, such as the proliferator activated receptor γ (PPARγ) and CCAAT/enhancer binding protein (C/EBPα) expressions, have been down regulated by chia seed treatment. Macrophage treated with chia seed-treated adipocyte condition media significantly inhibited the obesity associated inflammatory genes and protein expression levels, such as monocyte chemo attractant protein-1 (MCP-1), prostaglandins E2, interleukin-6, plasminogen activator inhibitor-1 (PAI-1) and tumor necrosis factor-α (TNF-α). In conclusion, a 3:1 ratio of omega 3 and omega 6 fatty acid composition of chia seed fatty acid content potentially inhibits lipid accumulation, and enhanced fatty acid oxidation, via UCP-1 and PRDM16 expression. Macrophage recruitment to adipocyte and the development of obesity associated inflammation was suppressed by chia seeds.

show abstract

“…When such cell types have been used to examine the ability of green tea compounds to induce the expression of UCP1 and other genes related to thermogenic potential, positive effects of these compounds have been reported (11,17), i.e., results opposite to those presented herein primary cultures of both brown and white adipocytes. We are not aware of studies with green tea compounds in other established white adipocyte-like cell lines such as C3H10T1/2 or hMADS cells, and thus, we cannot say whether similar outcomes would be observed in all white adipocyte-like cell lines.…”

Section: Established Adipogenic Cell Lines May Not Reflect the Phenotype And Epigenetics Of Primary Cellsmentioning

confidence: 96%

“…These observations are not without precedence in studies in adipocyte-like cell cultures. Several studies have reported an inhibitory effect of GTEs or polyphenols from green tea on adipogenesis in vitro (17,35). Kim et al (17) found that different catechins and gallic acid inhibited the accumulation of intracellular lipids in the 3T3-L1 cell line.…”

Section: Green Tea Compounds Are Negative For Adipogenesismentioning

confidence: 99%

See 1 more Smart Citation

On the Validity of Adipogenic Cell Lines as Model Systems for Browning Processes: In Authentic Brown, Brite/Beige, and White Preadipocytes, There is No Cell-Autonomous Thermogenic Recruitment by Green Tea Compounds

et al. 2021

View full text Add to dashboard Cite

The potential ability of nutritional compounds to induce or enhance the browning of adipocytes has attracted large interest as a workable means of combatting the obesity epidemic. Green tea compounds are discussed as such inducers of an enhanced thermogenic capacity and activity. However, the cell-autonomous effects of green tea compounds on adipocytes have until now only been demonstrated in adipogenic cell lines (3T3-L1 and 3T3-F442A), i.e., cells of undefined tissue lineage. In this study, we examine the ability of green tea compounds to cell-autonomously induce thermogenic recruitment in authentic brown and brite/beige adipocytes in vitro. In primary brown adipocytes, the green tea compounds suppressed basal UCP1 gene expression, and there was no positive interaction between the compounds and adrenergic stimulation. In white adipocytes, green tea compounds decreased both basal and norepinephrine-induced UCP1 mRNA levels, and this was associated with the suppression of cell differentiation, indicated by reduced lipogenic gene expression and lipid accumulation. A lack of interaction between rosiglitazone and green tea compounds suggests that the green tea compounds do not directly interact with the PPARγ pathway. We conclude that there is a negative effect of the green tea compounds on basal UCP1 gene expression, in both brown and white primary adipocytes, in contrast to the positive effects earlier reported from studies in adipogenic cell lines. We posit that the epigenetic status of the adipogenic cell lines is fundamentally different from that of genuine brown and white adipocytes, reflected, e.g., in several-thousand-fold differences in UCP1 gene expression levels. Thus, results obtained with adipogenic cell lines cannot unreservedly be extrapolated as being relevant for authentic effects in brown and white adipocytes. We suggest that this conclusion can be of general concern for studies attempting to establish physiologically relevant cell-autonomous effects.

show abstract

Epigallocatechin Exerts Anti‐Obesity Effect in Brown Adipose Tissue

Cited by 9 publications

References 26 publications

Epigoitrin alleviates lipid and glucose metabolic disorders induced by a high-fat diet

Epigoitrin alleviates lipid and glucose metabolic disorders induced by a high-fat diet

Beneficial Fatty Acid Ratio of Salvia hispanica L. (Chia Seed) Potentially Inhibits Adipocyte Hypertrophy, and Decreases Adipokines Expression and Inflammation in Macrophage

On the Validity of Adipogenic Cell Lines as Model Systems for Browning Processes: In Authentic Brown, Brite/Beige, and White Preadipocytes, There is No Cell-Autonomous Thermogenic Recruitment by Green Tea Compounds

Contact Info

Product

Resources

About