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Pandian, T. J.; Koteeswaran, R.

doi:10.1023/a:1003332526659

Cited by 238 publications

(36 citation statements)

References 326 publications

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“…Thus, gynogenetic diploid and wild-type triploid progeny occur in gynogenetically activated and normally fertilized eggs, respectively [2,3]. In the present study, both triploid and diploid wild-type progeny were also seen in the cold-shocked groups after normal fertilization.…”

Section: Discussionsupporting

confidence: 58%

“…Genetic inactivation of egg nucleus has typically been achieved by means of irradiating the eggs with gamma- and X-rays, but more recently it has been shown that the egg nucleus can be successfully inactivated using ultraviolet (UV) irradiation, especially in fish with relatively smaller egg sizes [1-4]. In aquaculture, cloning, rapid fixation of genotypes and sex-control (especially using super-male with YY genotype) are usually proposed by using genomes of completely homozygous doubled haploids, which are induced by chromosome duplication (endomitosis) at the first cleavage after the initiation of androgenetic development of haploid embryos [2,3]. Duplication of chromosomes in haploids is induced by temperature or pressure shock applied at the optimum timing of the pro-metaphase of the first mitotic cell division [5].…”

Section: Introductionmentioning

confidence: 99%

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Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish

et al. 2011

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BackgroundAndrogenesis (all-male inheritance) is generally induced by means of irradiating the eggs to inactivate the maternal genome, followed by fertilization with normal sperm. In fish, the conventional technique for induced androgenesis has been applied for rapid fixation to traits, recovery of cryopreserved genotypes, sex-control, etc. A new method of androgenesis that eliminates the need to irradiate the egg was proposed using the loach, Misgurnus anguillicaudatus (a teleost fish).ResultsWhen the eggs of wild-type females were fertilized with sperm of albino or orange phenotype males and cold-shocked at 0 to 3°C for 60 min duration just after fertilization, generally more than 30% (with a peak of 100%) of the hatched progeny were androgenotes. While a few of them were the normal diploid, most of them turned out to be abnormal haploid. All-male inheritance was verified by the expression of the recessive color trait (albino or orange) and microsatellite genotypes comprising only paternally derived alleles. Nuclear behavior after the cold-shock treatment was traced by microscopic observation of DAPI (4'6-diamidino-2-phenylindole)-stained samples and hematoxylin-eosin stained histological sections, and the extrusion of egg (maternal) nucleus was observed in eggs treated in the optimum timing.ConclusionIn this paper, we demonstrate that cold-shock treatment (at 0 and 3°C) of loach eggs for 60 min just after fertilization successfully induces androgenetic haploid development. The most likely mechanism of cold-shock induced androgenesis is an elimination of the egg nucleus together along with the second polar body and subsequent development of a decondensed sperm nucleus or male pronucleus.

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Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish

et al. 2011

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“…Triplods and one tetraploid male were found to produce sperm with highly variable DNA content (Flajšhans et al 2008). Data on spermiogenesis in artificially induced triploids (Pandian and Koteeswaran 1998) revealed that the mobility of aneuploid spermatozoa of triploid males was lower than that of euploid cells. Spermatozoa viability of diploid and triploid males was higher that of the tetraploid, however the percentage of motile spermatozoa in triploids was significantly lower in comparison with tetraploids and diploids (Flajšhans et al 2007(Flajšhans et al , 2008.…”

Section: Discussionmentioning

confidence: 99%

Karyotype, morphology, and reproduction ability of the Prussian carp, <I>Carassius gibelio</I> (Actinopterygii: Cypriniformes: Cyprinidae), from unisexual and bisexual populations in Poland

Boroń¹,

Szlachciak²,

Juchno³

et al. 2011

Acta Icth et Piscat

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“…Moreover, because triploidy can facilitate hybridization in otherwise reproductively isolated salmonids (e.g. triploid S. salar can hybridize with chum salmon, Oncorhynchus keta ) [13], it is important to assess whether viable embryos can be produced in significant numbers in this autotetraploid species.…”

Section: Introductionmentioning

confidence: 99%

Comparisons of reproductive function and fatty acid fillet quality between triploid and diploid farm Atlantic salmon ( Salmo salar )

et al. 2018

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Triploidy could prevent escaped farm salmon breeding in the wild, while also improving nutrient quality within farmed fillets. Despite these potential advantages, triploid Atlantic salmon have not been widely used in aquaculture, and their reproductive function has yet to be fully evaluated. Here, we compare reproductive function and fillet composition between triploid and diploid farm salmon under standard aquaculture rearing conditions. We show that female triploids are sterile and do not develop gonads. By contrast, males produce large numbers of motile spermatozoa capable of fertilizing wild salmon eggs. However, compared with diploids, reproductive development and survival rates of eggs fertilized by triploid males were significantly reduced, with less than 1% of eggs sired by triploid males reaching late-eyed stages of development. Analyses of fillets showed that total lipid and fatty acid quantities were significantly lower in triploid than in diploid Atlantic salmon fillets. However, when fatty acids were normalized to total lipid content, triploid fillets had significantly higher relative levels of important omega-3 long-chain polyunsaturated fatty acids. Our results show that: (i) escaped triploid farm salmon are very unlikely to reproduce in the wild and (ii) if able to match diploid fillet lipid content, triploid farm salmon could achieve better fillet quality in terms of essential fatty acids.

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Cited by 238 publications

References 326 publications

Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish

Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish

Karyotype, morphology, and reproduction ability of the Prussian carp, <I>Carassius gibelio</I> (Actinopterygii: Cypriniformes: Cyprinidae), from unisexual and bisexual populations in Poland

Comparisons of reproductive function and fatty acid fillet quality between triploid and diploid farm Atlantic salmon ( Salmo salar )

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