Karyotype, morphology, and reproduction ability of the Prussian carp, &lt;I&gt;Carassius gibelio&lt;/I&gt; (Actinopterygii: Cypriniformes: Cyprinidae), from unisexual and bisexual populations in Poland

Boroń, Alicja; Szlachciak, Jolanta; Juchno, Dorota; Grabowska, Anna; Jagusztyn, Bartosz; Porycka, Katarzyna

doi:10.3750/aip2011.41.1.04

Cited by 13 publications

(10 citation statements)

References 19 publications

(26 reference statements)

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“…The reason for this could be that the karyotype of the crucian carp contains a lot of very small chromosomes which are similar in size. The problem mainly concerns discrimination between sm and sta chromosomes as it occurs in the karyotype of a related species Carassius gibelio ( Boroń et al 2011 ). The karyotype obtained in the present study with a larger number (56) of biarmed than (44) of uniarmed chromosomes is the same as that supposed by Knytl et al (2013a , b ).…”

Section: Discussionmentioning

confidence: 99%

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Molecular cytogenetic analysis of the crucian carp, Carassius carassius (Linnaeus, 1758) (Teleostei, Cyprinidae), using chromosome staining and fluorescence in situ hybridisation with rDNA probes

Spóz¹,

Boroń²,

Porycka³

et al. 2014

CCG

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The crucian carp Carassius carassius (Linnaeus, 1758) is a species with restricted and decreasing distribution in Europe. Six males and six females of the species from the Baltic Sea basin in Poland were examined to show sequentially CMA3/AgNO3 staining pattern, DAPI staining, and, for the first time in literature, molecular cytogenetic analysis using double-colour fluorescence in situ hybridisation (FISH) with 28S and 5S rDNA probes. The karyotype consisted of 20 m, 36 sm and 44 sta chromosomes, NF=156. The AgNO3 stained NORs were most frequently located terminally in the short arms of two sm and two sta elements, and CMA3-positive sites were also observed suggesting abundant GC-rich repetitive DNA in the regions. Other CMA3-positive sites in the short arms of six to ten sm and sta chromosomes were detected. The results based on 28S rDNA FISH confirmed the location of rDNA sites. DAPI-negative staining of NORs suggested the scarcity of AT-rich DNA in the regions. FISH with 5S rDNA probe revealed 8–14 loci (ten and 12 in respectively 49 and 29% of metaphases). They were located in two sm and eight to ten sta chromosomes and six of them were larger than others. Simultaneously, mapping of the two rDNA families on the chromosomes of C. carassius revealed that both 28S and 5S rDNA probes were located in different chromosomes. Molecular cytogenetic data of C. carassius presented here for the first time give an important insight into the structure of chromosomes of this polyploid and declining species and may be useful in its systematics.

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Section: Discussionmentioning

confidence: 99%

“…Mitotic chromosome preparations were made from each individual following Boron et al (2011) . First, live fish were injected with a dose of 1ml of 0.05% colchicine solution per 100g body weight.…”

Section: Methodsmentioning

confidence: 99%

Molecular cytogenetic analysis of the crucian carp, Carassius carassius (Linnaeus, 1758) (Teleostei, Cyprinidae), using chromosome staining and fluorescence in situ hybridisation with rDNA probes

Spóz¹,

Boroń²,

Porycka³

et al. 2014

CCG

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“…All specimens were analysed cytogenetically in order to determine their ploidy level and the karyotype (not presented here; see Boroń et al, 2011 for details). Chromosome slides were made from kidney cells using conventional hypotonic treatment and methanol-acetic fixation following the standard air-drying technique as shown in detail by Boroń et al (2011). Fin clips of each individual were preserved in 96% ethanol and stored at -20% for further DNA extraction.…”

Section: Samplingmentioning

confidence: 99%

“…A wide spectrum of ecological tolerance and extraordinary resistance to adverse environmental conditions enabled C. gibelio to successively and progressively spread to various water bodies (Kottelat & Freyhof, 2007;Grabowska, Kotusz, & Witkowski, 2010;Boroń et al, 2011). Moreover, this species frequently hybridizes with other cyprinids, especially with the crucian carp, Carassius carassius (Linnaeus, 1758), which is native in Europe as well as with C. auratus and common carp, Cyprinus carpio (Linnaeus, 1758) (Sayer et al, 2011;Mezhzerin, Kokodii, Kulish, Verlatii, & Fedorenko, 2012;Wouters, Janson, Lusková, & Olsén, 2012;Rylková & Kalous, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Szabelska¹,

Kirtiklis²,

Przybył³

et al. 2017

Turk. J. Fish. Aquat. Sci.

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The Prussian carp may occur as diploid (2n=100) and/or triploid (3n=150) individuals, co-existing in many natural populations. The simultaneous occurrence of individuals with different ploidy makes the taxonomy of this species unclear. Additionally, the taxonomic status of C. gibelio has become even more enigmatic due to its hybridizing with other nonindigenous cyprinids. Since the variation within 5S rDNA can serve as a suitable marker for molecular identification of the fish ploidy, the main aim of present study was to compare this rDNA sequence between Prussian carp individuals with different ploidy levels: diploids (2n =100) and triploids (3n=150-160). PCR amplification of 5S rDNA generated two bands of approximately 340 and 470 bp in length (in both diploids and triploids) and band 200 bp visible in some individuals. These results indicate the presence of at least two different classes of 5S rRNA gene. Analysis of their nucleotide composition revealed no differences within the class of 340 bp and several nucleotide differences within the class of 470 bp between diploid and triploid individuals. The 5S rDNA variability detected in this study indicates the potential usefulness of this sequence for the identification of diploid and triploid individuals of the Prussian carp.

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“…This species is widely distributed in Europe, Siberia, and Asia; in the eastern regions, it is represented by bisexual populations with single monosex polyploid populations (Fan and Liu 1990). Most European populations are monosex populations, living as triploid females that reproduce gynogenetically (Boroń et al 2011), and such a population was chosen for this study to eliminate the effect of sex on the accumulation or elimination of lead during the experiment.…”

Section: Introductionmentioning

confidence: 99%

Lead accumulation and elimination in tissues of Prussian carp, Carassius gibelio (Bloch, 1782), after long-term dietary exposure, and depuration periods

Łuszczek‐Trojnar

Drąg‐Kozak

Popek

2012

Environ Sci Pollut Res

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We studied the bioaccumulation of lead in selected tissues of Prussian carp Carassius gibelio (Bloch, 1782) during 12 and 24 months exposure to different doses of this metal in feed and the elimination of lead from tissues during the following 12-month depuration period. Lead concentration was determined using atomic absorption spectrometry method. The highest lead concentrations were observed at 2.0 ± 0.54 to 7.4 ± 1.1 mg kg−1 in the kidney, 3.0 ± 0.13 to 5.2 ± 0.17 mg kg−1 in the bone, and 4.5 (±0.4) mg kg−1 in the hepatopancreas of fish from groups exposed to lead dietary concentration from 8 to 49 mg kg−1 for 24 months. The rate of accumulation were generally the highest at the beginning of exposure as evidenced by the highest monthly increments of bioaccumulation observed after 3 months of contamination for muscles, hepatopancreatic gland, intestine, and gills. Also analysis of the monthly increments of lead bioaccumulation in bone tissue and the highly significant coefficients of correlation indicate that the dynamics of accumulation are clearly dependent on dose of exposure. Depuration of accumulated lead from the organs depended mainly on tissue and duration of elimination period. Very rapid depuration was observed in soft tissues such as the intestine or muscles. Very low elimination was observed for scales and bones where until the end of the experiment highly significant lead concentration differences were observed in all groups in relation to the control group. Chronic dietary exposure in the range of 8–49 mg Pb kg−1 resulted in no significant effects on the growth and survival of Prussian carp females.Electronic supplementary materialThe online version of this article (doi:10.1007/s11356-012-1210-8) contains supplementary material, which is available to authorized users.

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Karyotype, morphology, and reproduction ability of the Prussian carp, <I>Carassius gibelio</I> (Actinopterygii: Cypriniformes: Cyprinidae), from unisexual and bisexual populations in Poland

Cited by 13 publications

References 19 publications

Molecular cytogenetic analysis of the crucian carp, Carassius carassius (Linnaeus, 1758) (Teleostei, Cyprinidae), using chromosome staining and fluorescence in situ hybridisation with rDNA probes

Molecular cytogenetic analysis of the crucian carp, Carassius carassius (Linnaeus, 1758) (Teleostei, Cyprinidae), using chromosome staining and fluorescence in situ hybridisation with rDNA probes

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Lead accumulation and elimination in tissues of Prussian carp, Carassius gibelio (Bloch, 1782), after long-term dietary exposure, and depuration periods

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