5-Methylcytosine in heterochromatic regions of chromosomes in Bovidae

Schnedl, W.; Erlanger, Bernard F.; Miller, Orlando J.

doi:10.1007/bf00270395

Cited by 33 publications

(22 citation statements)

References 22 publications

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“…3Bi). The large intense foci have been attributed to pericentromeric heterochromatin in bovine somatic cells (26). Because there are fewer foci than there are centromeres, this finding means that centromeres of different chromosomes are probably colocalized in specific areas.…”

Section: Conservation Of Methylationmentioning

confidence: 93%

Conservation of methylation reprogramming in mammalian development: Aberrant reprogramming in cloned embryos

Dean

Santos

Stojković

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

878

651

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Mouse embryos undergo genome-wide methylation reprogramming by demethylation in early preimplantation development, followed by remethylation thereafter. Here we show that genomewide reprogramming is conserved in several mammalian species and ask whether it also occurs in embryos cloned with the use of highly methylated somatic donor nuclei. Normal bovine, rat, and pig zygotes showed a demethylated paternal genome, suggesting active demethylation. In bovine embryos methylation was further reduced during cleavage up to the eight-cell stage, and this reduction in methylation was followed by de novo methylation by the 16-cell stage. In cloned one-cell embryos there was a reduction in methylation consistent with active demethylation, but no further demethylation occurred subsequently. Instead, de novo methylation and nuclear reorganization of methylation patterns resembling those of differentiated cells occurred precociously in many cloned embryos. Cloned, but not normal, morulae had highly methylated nuclei in all blastomeres that resembled those of the fibroblast donor cells. Our study shows that epigenetic reprogramming occurs aberrantly in most cloned embryos; incomplete reprogramming may contribute to the low efficiency of cloning.

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Section: Conservation Of Methylationmentioning

confidence: 93%

Conservation of methylation reprogramming in mammalian development: Aberrant reprogramming in cloned embryos

Dean

Santos

Stojković

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

878

651

View full text Add to dashboard Cite

show abstract

“…Miller to the chromosomes of several mammalian species, including human, chimpanzee, gorilla, cattle, mouse, and kangaroo rat Schreck et al, 1974Schreck et al, , 1977Schnedl et al, 1975Schnedl et al, , 1976. Using an immunofluorescence technique and anti-5-MeC antibodies, they showed that methylated DNA can be detected in fixed metaphase chromosomes after they have been UV-irradiated to generate regions of single-stranded DNA.…”

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confidence: 99%

Hypermethylated Chromosome Regions in Nine Fish Species with Heteromorphic Sex Chromosomes

Schmid

Steinlein

Yano

et al. 2015

Cytogenet Genome Res

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Sites and amounts of 5-methylcytosine (5-MeC)-rich chromosome regions were detected in the karyotypes of 9 Brazilian species of Characiformes fishes by indirect immunofluorescence using a monoclonal anti-5-MeC antibody. These species, belonging to the genera Leporinus, Triportheus and Hoplias, are characterized by highly differentiated and heteromorphic ZW and XY sex chromosomes. In all species, the hypermethylated regions are confined to constitutive heterochromatin. The number and chromosome locations of hypermethylated heterochromatic regions in the karyotypes are constant and species-specific. Generally, heterochromatic regions that are darkly stained by the C-banding technique are distinctly hypermethylated, but several of the brightly fluorescing hypermethylated regions merely exhibit moderate or faint C-banding. The ZW and XY sex chromosomes of all 9 analyzed species also show species-specific heterochromatin hypermethylation patterns. The analysis of 5-MeC-rich chromosome regions contributes valuable data for comparative cytogenetics of closely related species and highlights the dynamic process of differentiation operating in the repetitive DNA fraction of sex chromosomes.

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“…because anti nucleoside antibodies react only with single strand ed DNA. This method denatures AT-rich sequences to a somewhat greater extent than GC-rich se quences but has been used successfully to demon strate hypermethylation of not only AT-rich satel lite DNAs (M iller et al, 1974) but also GC-rich satellite DNAs of Bovidae (Schnedl et al, 1976). as well as the GC-rich inactive rRNA genes (Tan travaiii et al, 1981).…”

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confidence: 99%

Is DNA methylation responsible for mammalian X chromosome inactivation?

Miller

Okamoto

Erlanger³

et al. 1982

Cytogenet Genome Res

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Mohandas et al. (1981) have proposed that mammalian X chromosome inactivation involves segmental methylation of cytosine residues in the DNA at multiple sites along the X chromosome. Using antibodies specific for 5-methylcytosine, we have found no detectable difference in the extent of methylation of the DNA in the two X chromosomes of owl monkey or human females. Thus inactivation (facultative heterochromatization) of the X chromosome is not due to, or associated with, intense DNA methylation comparable to that seen in most constitutive heterochromatin.

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5-Methylcytosine in heterochromatic regions of chromosomes in Bovidae

Cited by 33 publications

References 22 publications

Conservation of methylation reprogramming in mammalian development: Aberrant reprogramming in cloned embryos

Conservation of methylation reprogramming in mammalian development: Aberrant reprogramming in cloned embryos

Hypermethylated Chromosome Regions in Nine Fish Species with Heteromorphic Sex Chromosomes

Is DNA methylation responsible for mammalian X chromosome inactivation?

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