5 Biochemical and Serological Characterization of Erwinia

Slade, Martin B.; Tiffin, A. I.

doi:10.1016/s0580-9517(08)70461-6

Cited by 24 publications

(19 citation statements)

References 166 publications

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“…However, the exact mechanism by which P. agglomerans suppresses diseases is not clear. Competition for nutrients and infection sites (Hatting et al 1986;Kempf and Wolf 1989), inhibition of other plant pathogens by producing acid conditions (Beer et al 1984), induction of plant defence (Slade and Tiffin 1984), parasitism (Bryk et al 1998), and the most common production of antimicrobial compounds (Beer et al 1984;Vanneste et al 1992) have been suggested as mechanisms by which P. agglomerans interacts as a biocontrol agent.…”

Section: Discussionmentioning

confidence: 99%

Post-harvest biological control by Pantoea agglomerans (CPA-2) on Golden Delicious apples

Nunes¹,

Usall²,

Teixidó³

et al. 2002

J Appl Microbiol

100

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Aims: To investigate the potential of Pantoea agglomerans to control the major post-harvest diseases on Golden Delicious apples. Methods and Results: In laboratory trials, a high level of control of Penicillium expansum, Botrytis cinerea and Rhizopus stolonifer was obtained with P. agglomerans. In semi-commercial trials at 1°C in air and a low oxygen atmosphere, the reduction of blue mould was 81% and 100%, respectively, and control of grey mould was achieved equally with P. agglomerans and imazalil. In trials at 1°C and seven atmosphere conditions, maximum reduction in decay was 80% obtained at 3% O 2 -6% CO 2 . The population of P. agglomerans on apples followed the same pattern under all three atmosphere conditions studied. Conclusions: Pantoea agglomerans could be used effectively on apples under a wide range of temperature and atmosphere conditions. Significance and Impact of the Study: Pantoea agglomerans can be used as a biocontrol agent on apples at 8 · 10 7 cfu ml -1, the same concentration as in pears. This will facilitate the application of this biological control agent by the growers in packing houses.

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Section: Discussionmentioning

confidence: 99%

Post-harvest biological control by Pantoea agglomerans (CPA-2) on Golden Delicious apples

Nunes¹,

Usall²,

Teixidó³

et al. 2002

J Appl Microbiol

100

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“…Several attempts have been made to clarify the taxonomic structure of Erwinia; these have been based on different methodologies, such as deoxyribonucleic acid (DNA)-DNA hybridization (2, &lo, 29,32,47), comparative phenotypic studies (17,(21)(22)(23)(24)(25)27,30,45,67), serological studies (11,19,59,60,72), gel electrophoresis of proteins (43,44,46,69), and characterization of the respiratory quinones and enzymes of fumarate metabolism (32). The problems related to the taxonomy of Erwinia have been discussed extensively (5 , 41, 45, 63, 65).…”

Section: (58)mentioning

confidence: 99%

Genus Erwinia: Numerical Analysis of Phenotypic Features

Verdonck

Mergaert

Rijckaert

et al. 1987

International Journal of Systematic Bacteriology

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API 20E and API SOCHE systems (72 phenotypic tests) were applied to a total of 529 strains, including 421 strains belonging to 21 different Erwinia species, 66 Enterobacter agglomerans strains, 18 Escherichia adecarboxylata strains, and 24 strains of 16 other enterobacteria. The results were analyzed numerically by using the Gower similarity coefficient and the unweighted average linkage method. The named Erwinia strains were distributed over 27 phena, some of which also contained strains received as Enterobacter agglomerans. Strains of Erwinia amylovora, Erwinia chrysanthemi, Erwinia cypripedii, Erwinia mallotivora, Erwinia nigrijiuens, Erwinia paradisiaca, Erwinia quercina, Erwinia rubrifaciens, Erwinia salicis, Erwinia stewartii, and Escherichia adecarboxylata constitute separate phena. Erwinia carotovora, Erwinia chrysanthemi, and Erwinia rhapontici are heterogeneous, but distinct from each other and from the other phena. The type strains of Erwinia herbicola, Enterobacter agglomerans, and Erwinia milletiae fall into one phenon, and strains of Erwinia ananas and Erwinia uredovora are in a single phenon. Obviously misnamed Erwinia herbicola and Enterobacter agglomerans strains can be assigned to other species, such as Erwinia cypripedii, Erwinia ananas, Erwinia rhapontici, Rahnella aquatilis, Enterobacter sakazakii, Escherichia adecarboxyluta, and Serratia marcescens or to as-yet-unnamed phena. Three Erwinia carnegieana strains, but not the type strain, form one phenon. Erwinia dissolvens and Erwinia nimipressuralis should be allocated to Enterobacter. Our results confirm the heterogeneous taxonomic structure of the genus Erwinia.According to Lelliott and Dickey (41), the genus Erwinia consists of 15 species that are associated with plants as pathogens, saprophytes, or epiphytes. Erwinia herbicola also includes human clinical isolates. There are 21 Erwinia species names on the Approved Lists of Bacterial Names (58).Several attempts have been made to clarify the taxonomic structure of Erwinia; these have been based on different methodologies, such as deoxyribonucleic acid (DNA)-DNA hybridization (2, &lo, 29,32,47), comparative phenotypic studies (17,(21)(22)(23)(24)(25)27,30,45,67), serological studies (11,19,59,60,72), gel electrophoresis of proteins (43,44,46,69), and characterization of the respiratory quinones and enzymes of fumarate metabolism (32). The problems related to the taxonomy of Erwinia have been discussed extensively (5 , 41, 45, 63, 65). These problems are based mainly on the heterogeneity of the genus Erwinia, as reflected in (i) the (former) subdivision of Erwinia into three "natural groups" based on phytopathological characteristics (21-25, 40), (ii) the unclear delineation of some species, such as Erwinia carotovora and Erwinia herbicola, due to their internal heterogeneity (4, 45, 63, 65), and (iii) the existence of an "Erwinia herbicola-Enterobacter agglomerans complex," with unclear relationships among its members and with other taxa of the Enterobacteriaceae (4).Because of the inadequa...

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“…Plant Pathol., in press). Unlike most of the' soft rot erwinias, E. chrysanthemi giyes rise to a systemic disease (32). Systemic disease symptoms occur'when the bacteria express virulence upon spreading through the vascular system of the plant.…”

mentioning

confidence: 99%

Systemic virulence of Erwinia chrysanthemi 3937 requires a functional iron assimilation system

Enard¹,

Diolez²,

Expert³

1988

J Bacteriol

156

113

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In Erwinia chrysanthemi, conditions of iron starvation initiate production of a catechol-type siderophore and enhance production of three outer membrane polypeptides. Twenty-two mutants affected in the different stages of this iron assimilation system were isolated by mini-Mu insertion mutagenesis. All of them failed to induce systemic soft rot on axenically grown Saintpaulia plants. From the siderophore auxotrophs and the iron uptake mutants, clones having recovered the missing function(s) were isolated by using the in vivo cloning vector pULB113 (RP4::mini-Mu). An R-prime plasmid containing a ca. 35.5-kilobase-pair DNA insert was identified. Restoration of the iron functions restored partially, if not completely, the virulence of the parental strain.

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5 Biochemical and Serological Characterization of Erwinia

Cited by 24 publications

References 166 publications

Post-harvest biological control by Pantoea agglomerans (CPA-2) on Golden Delicious apples

Post-harvest biological control by Pantoea agglomerans (CPA-2) on Golden Delicious apples

Genus Erwinia: Numerical Analysis of Phenotypic Features

Systemic virulence of Erwinia chrysanthemi 3937 requires a functional iron assimilation system

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