45. Carbon-13 and Nitrogen-15 Turnover in Serum of Bubaline Donors of Biological Material for Medical Use

Silva, Daniela A. Fossato da; Biscola, Natália P.; Souza, Renato M.F.; Caetano, Denis A.; Denadai, Juliana Célia; Sartori, Maria Márcia Pereira; Silva, Evandro T. da; Ducatti, Carlos; Martins, Cyntia Ludovico; Jorge, A. M.; Santos, Lucilene Delazari dos; Ferreira, Rui Seabra; Barraviera, Benedito

doi:10.1016/j.toxicon.2012.04.046

Cited by 3 publications

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“…In suspicious cases, the necropsy becomes the highest priority, followed by anatomical-pathological analysis. Researchers at CEVAP in partnership with the Center for Stable Environmental Isotopes, in the Botucatu Biosciences Institute of UNESP, developed a globally pioneering technique of isotopic tracers based on the dosing of carbon isotopes ( 13 C) and nitrogen ( 15 N) in sheep and buffaloes [30, 31]. After its standardization, this technique was tested in animals of the abovementioned herd, showing an absence of animal protein ingestion, which indirectly indicates that the buffalo donors of cryoprecipitate were potentially free of mad cow disease.…”

Section: Composition Of the Heterologous Fibrin Sealantmentioning

confidence: 99%

Heterologous fibrin sealant derived from snake venom: from bench to bedside – an overview

Ferreira

Barros

Abbade

et al. 2017

J Venom Anim Toxins Incl Trop Dis

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Hemostatic and adhesive agents date back to World War II, when homologous fibrin sealant came onto scene. Considering that infectious diseases can be transmitted via human blood, a new heterologous fibrin sealant was standardized in the 1990s. Its components were a serine protease (a thrombin-like enzyme) extracted from the venom of Crotalus durissus terrificus snakes and a fibrinogen-rich cryoprecipitate extracted from the blood of Bubalus bubalis buffaloes. This new bioproduct has been used as a coagulant, sealant, adhesive and recently as a candidate scaffold for mesenchymal stem cells and bone and cartilage repair. This review discusses the composition of a new heterologous fibrin sealant, and cites published articles related to its preclinical applications aiming at repairing nervous system traumas and regenerating bone marrow. Finally, we present an innovative safety trial I/II that found the product to be a safe and clinically promising candidate for treating chronic venous ulcers. A multicenter clinical trial, phase II/III, with a larger number of participants will be performed to prove the efficacy of an innovative biopharmaceutical product derived from animal venom.Electronic supplementary materialThe online version of this article (doi:10.1186/s40409-017-0109-8) contains supplementary material, which is available to authorized users.

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Section: Composition Of the Heterologous Fibrin Sealantmentioning

confidence: 99%

Heterologous fibrin sealant derived from snake venom: from bench to bedside – an overview

Ferreira

Barros

Abbade

et al. 2017

J Venom Anim Toxins Incl Trop Dis

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“…Recently, the measurement of δ 13 C and δ 15 N using isotope-ratio mass spectrometry (IRMS) has been used successfully in the processes of disease control, authentication and certification of animal products [17-19], traceability [4,20,21] and evaluation of conventional and organic production systems for beef [17,22,23].…”

Section: Introductionmentioning

confidence: 99%

Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases

Júnior

Silva

Biscola

et al. 2019

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Ruminant feed containing animal byproduct proteins (ABPs) is prohibited in many countries due to its risk of transmitting prion diseases (PD). In most cases the entire herd is sacrificed, which causes great harm to the producer countries by preventing their exportation of ruminant derived-products. Methods: We used stable isotope ratio mass spectrometry (IRMS) of carbon ( 13 C/ 12 C) and nitrogen ( 15 N/ 14 N) to trace the animal protein in the blood of 15 buffaloes ( Bubalus bubalis ) divided into three experimental groups: 1 - received only vegetable protein (VP) during 117 days; 2 - received animal and vegetable protein (AVP); and 3 - received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3 received diets containing 13.7% bovine meat and bone meal (MBM) added to a vegetable diet (from days 21-117 in the AVP group and until day 47 in the AVPR group, when MBM was removed). Results: On the 36th day, differences were detectable in the feeding profile (p <0.01) among the three experimental groups, which remained for a further 49 days (85th day). The AVPR group showed isotopic rate reversibility on the 110th day by presenting values similar to those in the control group (VP) (p> 0.05), indicating that it took 63 days to eliminate MBM in this group. Total atoms exchange (> 95%) of 13 C and 15 N was observed through incorporation of the diet into the AVP and AVPR groups. Conclusions: IRMS is an accurate and sensitive technique for tracing the feeding profile of ruminants through blood analysis, thus enabling investigation of ABP use.

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“…However, there is a gap between the approach used to trace proteins and assessments of their tissue turnover rates (Bahar et al, 2009). Ascertaining the assimilation of stable isotopes in different tissues or biological fluids can define turnover rates, which depend on feeding times (Fossato da Silva et al, 2012;Martinez et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)

Silva

Biscola

Santos

et al. 2016

The Veterinary Journal

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Sheep are used in many countries as food and for manufacturing bioproducts. However, when these animals consume animal by-products (ABP), which is widely prohibited, there is a risk of transmitting scrapie - a fatal prion disease in human beings. Therefore, it is essential to develop sensitive methods to detect previous ABP intake to select safe animals for producing biopharmaceuticals. We used stable isotope ratio mass spectrometry (IRMS) for C andN to trace animal proteins in the serum of three groups of sheep: 1 - received only vegetable protein (VP) for 89 days; 2 - received animal and vegetable protein (AVP); and 3 - received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3 received diets with 30% bovine meat and bone meal (MBM) added to a vegetable diet (from days 16-89 in the AVP group and until day 49 in the AVPR group, when MBM was removed). The AVPR group showed N equilibrium 5 days after MBM removal (54th day). Conversely,N equilibrium in the AVP group occurred 22 days later (76th day). The half-life differed between these groups by 3.55 days. In the AVPR group, N elimination required 53 days, which was similar to this isotope's incorporation time. Turnover was determined based on naturalN signatures. IRMS followed by turnover calculations was used to evaluate the time period for the incorporation and elimination of animal protein in sheep serum. The δC and δN values were used to track animal protein in the diet. This method is biologically and economically relevant for the veterinary field because it can track protein over time or make a point assessment of animal feed with high sensitivity and resolution, providing a low-cost analysis coupled with fast detection. Isotopic profiles could be measured throughout the experimental period, demonstrating the potential to use the method for traceability and certification assessments.

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45. Carbon-13 and Nitrogen-15 Turnover in Serum of Bubaline Donors of Biological Material for Medical Use

Cited by 3 publications

References 0 publications

Heterologous fibrin sealant derived from snake venom: from bench to bedside – an overview

Heterologous fibrin sealant derived from snake venom: from bench to bedside – an overview

Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases

Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)

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