“…Besides reproductive changes, the possible detection of α-and β-ZEL is one of the reasons why the occurrence of ZEN has been constantly studied in pastures (Di Menna et al, 1987;Nichea et al, 2015;Reed and Moore, 2009;Salvat et al, 2013;Štýbnarová et al, 2016). In this context, the levels of ZEN reported by Burkin and Kononenko (2015), Di Menna et al (1987), Gott et al (2017Gott et al ( , 2018Gott et al ( , 2019, Nichea et al (2015), Reed et al (2004), and Reed and Moore (2009) were higher than the lowest concentration that cause physiological effects (greater than 1,000 µg/kg), as determined by Reed and Moore (2009). Besides, together with the results by Salvat et al (2013), these studies reported levels greater than the EU recommendations of 500 µg/kg for feed destined for calves, dairy cattle, sheep, and goats (European Commission, 2016).…”