41 B Asma Anacına In Vivo Kolhisin Uygulamalarının Morfolojik ve Sitolojik Etkileri

Kara, Zeki; Doğan, Osman; Yazar, Kevser; Sabır, Ali

doi:10.15316/sjafs.2018.57

Cited by 5 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Stomata area values were significantly differed due to the colchicine applications. A similar results of colchicine treatments was reported by Kara et al, (2018) on stomatal differences without genome doubling.…”

Section: Stomatal Density (Stomata MM -2 )supporting

confidence: 88%

Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage

Kara¹,

Sabır²,

Yazar³

et al. 2018

SJAFS

Self Cite

View full text Add to dashboard Cite

Section: Stomatal Density (Stomata MM -2 )supporting

confidence: 88%

Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage

Kara¹,

Sabır²,

Yazar³

et al. 2018

SJAFS

Self Cite

View full text Add to dashboard Cite

“…This difference is probably due to increased cell size of polyploid plants (Marinho et al, 2014) and/or decreased leaf mesophyll space (Lundgren et al, 2019). Although the stomatal characteristics of the selected plants in our study were like the results of previous studies (Kara et al, 2018a;Zeng et al, 2019;Bae et al, 2020), ploidy could not be confirmed.…”

Section: Stoma Density (Number MM -2 ) and Stoma Sizes (μM)contrasting

confidence: 75%

“…In surviving plants, stomatal density per unit area (number mm -2 ) (Kara et al, 2018c), stomatal sizes (μm) (Kara et al, 2018a), chloroplast numbers in stoma guard cells (number stoma -1 ) (Kara et al, 2018a;Yazar, 2021), leaf thickness, chlorophyll content (Kara et al, 2018b) and Flow Cytometry (FC) analyzes (Yazar, 2018) were done. According to the results of all analysed data, the effects of oryzalin and N2O treatments on chromosome folding in grapevine were evaluated.…”

Section: Methodsmentioning

confidence: 99%

Reactions of Some Grapevine Rootstock Cuttings to Mutagenic Applications

Kara¹,

Doğan²

2022

SJAFS

Self Cite

View full text Add to dashboard Cite

Grapevine (Vitis spp.) is one of the most important socio-economically important plants in the global scale, and the need for its breeding is increasing. In viticulture, mutation is more promising than crossbreeding in breeding new genotypes from natural genetic diversity. Polyploid plants outperform their diploid relatives in several respects. In this study, the efficacy of oryzalin and N2O mutagens in the induction of polyploidy was investigated by applying different doses and durations to the forced cuttings of 41B [Chasselas (Vitis vinifera L.) × Vitis berlandieri Planch)] and Fercal [(Vitis vinifera x Vitis berlandieri) × 333 EM] rootstocks. LD50 values of mutagen applications were determined, morphological and cytological effects were examined by macroscopic, microscopic, and cytological methods. Application time and dose increase of mutagens decreased LD50 values. As a result of mutagen applications, leaf thickness and chlorophyll content of the surviving plants increased. Applications increased stomatal sizes, decreased their density, increased chloroplast numbers, increased leaf thicknesses and partially SPAD values. It was determined that they were not polyploid in the confirmation test performed with flow cytometry (FC) analyses in 4 Fercal and 1 41B samples that were assumed to be mutant by stoma and chloroplast examinations. After that, it was thought that it would be appropriate to try vegetative material with actively dividing cells, such as nodal cuttings, in the studies of obtaining polyploid individuals on grapevine rootstocks

show abstract

“…This difference is probably due to an increase in cell size in polyploid individuals (Marinho et al 2014) and/or a decrease in leaf mesophyll porosities (Lundgren et al 2019). The stomatal characteristics (Bae et al 2020, Kara et al 2018a, Zeng et al 2019 and chloroplast numbers (Ewald et al 2009, Rao et al 2019, Rêgo et al 2011 of polyploid plants obtained in this study were like previous studies, and our results confirmed previous studies.…”

Section: Discussionsupporting

confidence: 90%

“…The nuclear DNA content of the Vitaceae family was between 0.62 and 1.24 pg (Chu et al 2018), nine different diploid Vitis vinifera varieties ranged from 1.17 to 1.26 (Leal et al 2006), diploid Tekirdag Muscat and Michele Palieri varieties were 1.10 and 1.20 (Ergönül et al 2018), and diploid 41 B grapevine rootstocks were reported as 1.028 pg (Kara et al 2018a). Gordej et al (2019) reported that the nuclear DNA content of tetraploid plants obtained because of N 2 O application increased 1.7 to 1.82-fold compared to diploids.…”

Section: Discussionmentioning

confidence: 99%

Mutagenic effects of nitrogen protoxide and oryzalin on “41 B” and “Fercal” grapevine rootstocks seedlings

Kara,

Doğan

2023

Breed. Sci.

Self Cite

View full text Add to dashboard Cite

In this study, the mutagenic effects of different doses and exposure times of oryzalin and Nitrogen Protoxide (N 2 O) were tested for stimulating polyploid on 41 B and Fercal grapevine rootstocks seedlings. Ploidy changes were examined by morphological, cytological, macroscopic, and microscopic methods. Leaf thickness, chlorophyll contents, stomatal sizes, and chloroplast numbers of polyploid seedlings stimulated with mutagens increased but their stomatal densities decreased. Flow cytometry (FC) analyses were performed on 50 samples selected by morphological and microscopic preliminary determinations. In FC analyses, 1 tetraploid seedling and 4 mixoploid seedlings from Fercal offspring and 1 mixoploid seedling from 41 B offspring were verified. The nuclear DNA content of tetraploid and mixoploid seedlings were increased by 2.00 and 1.34-fold, respectively, when compared to their diploid parents. Chromosome counts in root tip samples propagated in vitro from the tetraploid Fercal offspring confirmed a 2-fold increase compared to the diploid parent. In polyploidy induction studies, it was deemed appropriate to use FC analysis and chromosome count together to confirm the ploidy levels of mutants. Oryzalin and N 2 O applications at different doses and exposure times were found to be effective for inducing polyploidy in 41 B and Fercal grapevine rootstocks.

show abstract

41 B Asma Anacına In Vivo Kolhisin Uygulamalarının Morfolojik ve Sitolojik Etkileri

Cited by 5 publications

References 0 publications

Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage

Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage

Reactions of Some Grapevine Rootstock Cuttings to Mutagenic Applications

Mutagenic effects of nitrogen protoxide and oryzalin on “41 B” and “Fercal” grapevine rootstocks seedlings

Contact Info

Product

Resources

About