3D bioprinting of integral ADSCs-NO hydrogel scaffolds to promote severe burn wound healing

Abstract

Severe burns are challenging to heal and result in significant death throughout the world. Adipose-derived mesenchymal stem cells (ADSCs) have emerged as a promising treatment for full-thickness burn healing but are impeded by their low viability and efficiency after grafting in vivo. Nitric oxide (NO) is beneficial in promoting stem cell bioactivity, but whether it can function effectively in vivo is still largely unknown. In this study, we bioprinted an efficient biological scaffold loaded with ADSCs and NO (3D-ADSCs/NO) to evaluate its biological efficacy in promoting severe burn wound healing. The integral 3D-ADSCs/NO hydrogel scaffolds were constructed via 3D bioprinting. Our results shown that 3D-ADSCs/NO can enhance the migration and angiogenesis of Human Umbilical Vein Endothelial Cells (HUVECs). Burn wound healing experiments in mice revealed that 3D-ADSCs/NO accelerated the wound healing by promoting faster epithelialization and collagen deposition. Notably, immunohistochemistry of CD31 suggested an increase in neovascularization, supported by the upregulation of vascular endothelial growth factor (VEGF) mRNA in ADSCs in the 3D biosystem. These findings indicated that 3D-ADSC/NO hydrogel scaffold can promote severe burn wound healing through increased neovascularization via the VEGF signalling pathway. This scaffold may be considered a promising strategy for healing severe burns.

A sandwich structure composite wound dressing with firmly anchored silver nanoparticles for severe burn wound healing in a porcine model

“…The content of NO is low in the physiological state, and it increases when tissue damage occurs [ 55 ]. High concentrations of NO could lead to inflammatory response aggravated in the early stage of wound healing [ 56 ]. Moreover, a large amount of NO in the wound site could promote the excessive expansion of the blood vessels and exudate secretion, causing tissue ischemia and hypoxia.…”

The 3D Bioprinted Scaffolds for Wound Healing

“…The incorporation of a NO donor, such as S-Nitroso-Nacetyl-D, or L-penicillamine, successfully protects against ischaemia and reperfusion injury, and improves the proangiogenic potential of the cells. Indeed, the bioprinted scaffold effectively promotes wound healing in a severe burn model [238].…”

Recent Advances in Hydrogel-Mediated Nitric Oxide Delivery Systems Targeted for Wound Healing Applications

“…Chemical Structure GSNO S-nitrosogluthathione characteristic of the last stage of wound closure [68,69].…”

“…Due to this property, RSNOs have b linked to greater antibacterial activity as the result of nitrosylation of thiolated prote [58]. characteristic of the last stage of wound closure [68,69].…”