The 3D Bioprinted Scaffolds for Wound Healing

Antezana, Pablo Edmundo; Municoy, Sofía; Echazú, María Inés Alvarez; Santo-Orihuela, Pablo Luis; Catalano, Paolo N.; Al‐Tel, Taleb H.; Kadumudi, Firoz Babu; Dolatshahi-Pirouz, Alireza; Orive, Gorka; Desimone, Martín Federico

doi:10.3390/pharmaceutics14020464

Cited by 47 publications

(23 citation statements)

References 265 publications

(297 reference statements)

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“…Various collagen-based materials have been used, including particles, fibers, films, sponges, and hydrogels, which can act as cell-supporting scaffolds or cell encapsulating matrices [ 22 ]. However, loading these scaffolds with known healing plants can perfectly, replace skin grafts by providing immediate protection to the wound and improving the quality of tissue regeneration and by providing would area with the antioxidant and proliferative properties coming from plant extracts [ 23 ]. The combination between collagen biomaterials and vegetable sources is poorly studied despite they present a promising approach.…”

Section: Introductionmentioning

confidence: 99%

Design of 3D Hybrid Plant Extract/Marine and Bovine Collagen Matrixes as Potential Dermal Scaffolds for Skin Wound Healing

Lahmar

Rjab

Sioud

et al. 2022

The Scientific World Journal

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Tissue engineering involves the use of smart biomimetic hybrid matrices to reinforce the cellular interaction with the matrix and restore native properties after regeneration. In this study, we highlight the potential of 3D collagen sponges soaked with bioactive extract, to enhance the wound healing process in vivo. Acid-soluble collagen from two sources, marine and bovine, were extracted and characterized physiochemically using Fourier transform infrared spectroscopy (FTIR) and SDS-PAGE. Our results confirmed that the extracted collagens were mainly composed of collagen type I with slight molecular structure differences. Both collagens present two different α chains (α1 and α2) and one β chain. Highly interconnected 3D scaffolds from collagen from the skin are designed and added by the widely known healing plants Pistacia lentiscus and Calendula officinalis. The resulting 3D collagen matrices possess fine biocompatibility with skin cells, Hacat (keratinocytes), and 3T3-L1 (fibroblasts) cells. To evaluate the potential wound healing effect, a collagen sponge soaked with the bioactive extract was tested on BALB/c mice. Our findings confirmed that sponges significantly improve animal re-epithelialization by increasing wound closure. Consequently, spongy collagen scaffolds loaded with Pistacia lentiscus and Calendula officinalis could be used as potential wound dressing material.

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Section: Introductionmentioning

confidence: 99%

Design of 3D Hybrid Plant Extract/Marine and Bovine Collagen Matrixes as Potential Dermal Scaffolds for Skin Wound Healing

Lahmar

Rjab

Sioud

et al. 2022

The Scientific World Journal

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“…The mesh formed by 3D printing provides the hydrogel with a pore structure that supports better cell and tissue growth. In addition, the grid-like topology functions to guide the directional migration of cells and tissues ( Antezana et al, 2022 ). Based on the fast gelation property and shear thinning behavior, EWH was smoothly extruded from the needle tube and formed a standard mesh structure (2.5 cm x 2.5 cm x 0.2 cm) in the cell culture medium ( Figure 3A ).…”

Section: Resultsmentioning

confidence: 99%

Direct three-dimensional printed egg white hydrogel wound dressing promotes wound healing with hitching adipose stem cells

Wang

Niu

et al. 2022

Front. Bioeng. Biotechnol.

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Current wound dressing based on hydrogel offers a promising way to accelerate the healing process, yet great challenges remain in the development of a highly integrated and efficient platform with the combination of therapeutic biomolecules and stem cells. Herein, a natural hydrogel wound dressing from egg white can be conveniently obtained by feasible physical crosslinking, the prepared hydrogel dressing features interconnected microporous channels, direct 3D printing, cytocompatibility, and intrinsic biomolecules to advance cell behavior. The 3D printed egg white hydrogels promote the adhesion and proliferation of adipose-derived stem cells (ASCs) without obvious cytotoxicity. In addition, this integrated hydrogel platform accompanied with adipose-derived stem cells accelerates wound healing through the enhancement of fibroblast proliferation, angiogenesis, and collagen rearrangement in the wound bed. The egg white hydrogel provides an effective wound caring product possessing low cost, easy availability along with ready manufacturing, and advanced therapeutic effect, which may be extended for the management of chronic or other complicated wounds.

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“…The demonstration that the bioartificial skin could protect the patient’s inner tissues from the incoming UV light supports the clinical use of the UGRSKIN model. The suitability of this skin model should be compared with other types of skin substitutes generated by 3D printing or other biofabrication methods [ 47 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range

et al. 2022

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The most recent generation of bioengineered human skin allows for the efficient treatment of patients with severe skin defects. Despite UV sunlight can seriously affect human skin, the optical behavior in the UV range of skin models is still unexplored. In the present study, absorbance and transmittance of the UGRSKIN bioartificial skin substitute generated with human skin cells combined with fibrin-agarose biomaterials were evaluated for: UV-C (200–280 nm), -B (280–315 nm), and -A (315–400 nm) spectral range after 7, 14, 21 and 28 days of ex vivo development. The epidermis of the bioartificial skin substitute was able to mature and differentiate in a time-dependent manner, expressing relevant molecules able to absorb most of the incoming UV radiation. Absorbance spectral behavior of the skin substitutes showed similar patterns to control native skin (VAF > 99.4%), with values 0.85–0.90 times lower than control values at 7 and 14- days and 1.05–1.10 times the control values at 21- and 28-days. UV absorbance increased, and UV transmission decreased with culture time, and comparable results to the control were found at 21 and 28 days. These findings support the use of samples corresponding to 21 or 28 days of development for clinical purposes due to their higher histological similarities with native skin, but also because of their absorbance of UV radiation.

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The 3D Bioprinted Scaffolds for Wound Healing

Cited by 47 publications

References 265 publications

Design of 3D Hybrid Plant Extract/Marine and Bovine Collagen Matrixes as Potential Dermal Scaffolds for Skin Wound Healing

Design of 3D Hybrid Plant Extract/Marine and Bovine Collagen Matrixes as Potential Dermal Scaffolds for Skin Wound Healing

Direct three-dimensional printed egg white hydrogel wound dressing promotes wound healing with hitching adipose stem cells

Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range

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