A sandwich structure composite wound dressing with firmly anchored silver nanoparticles for severe burn wound healing in a porcine model

Abstract

Wounds may remain open for a few weeks in severe burns, which provide an entry point for pathogens and microorganisms invading. Thus, wound dressings with long-term antimicrobial activity are crucial for severe burn wound healing. Here, a sandwich structure composite wound dressing anchored with silver nanoparticles (AgNPs) was developed for severe burn wound healing. AgNPs were in situ synthesized on the fibers of chitosan nonwoven fabric (CSNWF) as the interlayer of wound dressing for sustained release of silver ion. The firmly anchored AgNPs could prevent its entry into the body, thereby eliminating the toxicity of nanomaterials. The outer layer was a polyurethane membrane, which has a nanoporous structure that could maintain free transmission of water vapor. Chitosan/collagen sponge was selected as the inner layer because of its excellent biocompatibility and biodegradability. The presence of AgNPs in the CSNWF was fully characterized, and the high antibacterial activity of CSNWF/AgNPs was confirmed by against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The superior wound healing effect on deep dermal burns of presented composite wound dressing was demonstrated in a porcine model. Our finding suggested that the prepared AgNPs doped sandwich structure composite wound dressing has great potential application in severe wound care.

Application Effect of Silver-Containing Dressings in the Repair of Chronic Refractory Wounds

“…Once in contact with wound exudate, the hydrocolloid particle (CMC sodium carboxymethylcellulose) in the dressing swells after absorbing the exudate, forms a soft, cohesive hydrogel that allows it to absorb the wound exudate while locking it in within the gel, avoiding the risk of exudate leakage as well as the wound skin. Intrasite gel also creates a moist environment conducive to wound healing [20][21][22]. e results of this study showed that after treatment, the positive rate of wound bacterial culture in the observation group was significantly lower than that in the control group.…”

“…Once in contact with wound exudate, the hydrocolloid particle (CMC sodium carboxymethylcellulose) in the dressing swells after absorbing the exudate, forms a soft, cohesive hydrogel that allows it to absorb the wound exudate while locking it in place within the gel, avoiding the risk of exudate leakage as well as macerating the wound skin. Intrasite gel also creates a moist environment conducive to wound healing [ 20 – 22 ].…”

Biomimetic Asymmetric Composite Dressing by Electrospinning with Aligned Nanofibrous and Micropatterned Structures for Severe Burn Wound Healing

“…Wound infections are a major challenge in burn care, and severe bacterial infections can result in bacteremia, endocarditis, and high mortality. Currently, antibiotics are widely used to treat bacterial infections; however, the misuse can result in higher bacterial resistance and cross-resistance to clinically important antibiotics. , There is an urgent need to explore new and effective strategies for reducing bacterial drug resistance and preventing bacterial infections. Inspired by the lotus leaf effect, the construction of superhydrophobic surfaces with hierarchical micronanostructures is considered an effective option for preventing bacterial infections.…”

An Advanced Review: Polyurethane-Related Dressings for Skin Wound Repair

“…Functional exploration of polyurethane dressings has primarily used rat, mouse, and rabbit models, with wound repair models from pigs being rarely used [116]. The main disadvantages of using rabbit or rodent models are the differences in skin physiology, healing patterns, and skin-attached hairs.…”