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Bronsema, F.B.F.; Oostveen, W.J.F. van; Lammeren, A.A.M. van

doi:10.1023/a:1010605519845

Cited by 22 publications

(4 citation statements)

References 13 publications

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“…It might be connected with more rapid depletion of auxin during the 2-week subculture. Remotti (1995) and Bronsema et al (2001) link the development of somatic embryos on media with high auxin concentrations to a decrease in its amount during the culture. Thus, by a proper choice of inoculum density in correlation with other factors, including the growth regulator content of a medium, it is possible to influence morphogenesis.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of different liquid/solid culture systems in the production of somatic embryos from Narcissus L. ovary explants

Malik

2008

Plant Cell Tiss Organ Cult

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Alternative procedures for the production of Narcissus L. somatic embryos were investigated. Somatic embryogenesis was initiated on ovary explants isolated from cv. Carlton bulbs, chilled for 12 weeks at 5°C. The explants were cultured on MS media with 3% sucrose and growth regulators: Picloram or 2,4-D (10 or 25 lM) and BA (1 or 5 lM) for 12 weeks in the culture systems: continuous cultivation on solid media, continuous cultivation in liquid media and sequential cultivation using cycles in liquid and solid media. Two types of somatic embryogenesis, indirect and direct, were observed. The developmental pathway depended on the period of exposure to liquid media. Somatic embryos were formed via embryogenic nodular callus on solid media. 2,4-D and BA stimulated the process. The 4-week and 8-week liquid medium treatments resulted in the development of somatic embryos directly from the ovary explant tissue. The highest number of somatic embryos was noted under the influence of 25 lM 2,4-D and 5 lM BA in explants cultivated for 8 weeks in liquid medium and then, for 4 weeks, on solid medium. The effects of inoculum density on biomass increase and the formation of somatic embryos in cultures obtained on a medium with 25 lM 2,4-D and 5 lM BA were also checked. The highest biomass increase was observed after subculturing in liquid medium containing 0.5 lM NAA and 5 lM BA when the density of inoculum was 0.5 g/25 ml of the medium. The highest number of somatic embryos was noted when the density of inoculum was 1.5 g/25 ml.

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Section: Discussionmentioning

confidence: 99%

“…The reduction of auxin concentration in a medium was necessary for further development of Nerine and Zea somatic embryos (Lilien-Kipnis et al 1994;Bronsema et al 2001). It concerns especially the synthetic auxin (for example 2,4-D) which is less metabolized compared to other auxins (Von Arnold et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Comparison of different liquid/solid culture systems in the production of somatic embryos from Narcissus L. ovary explants

Malik

2008

Plant Cell Tiss Organ Cult

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“…The studies conducted by Nowakowska et al [75] and Mahato and Chaudhary [82] have emphasized that the efficiency of this process is influenced not only by the timing and method of hormone application but primarily by their concentration. However, it is important to remember that using high concentrations of 2,4-D in in vitro cultures is toxic to plants and can result in tissue necrosis [83] or the inhibition of embryo germination, as observed by Bronsema et al [84] in maize. Considering that synthetic auxins applied at high concentrations exhibit strong toxic properties, which could consequently result in low survival rates of oat haploid embryos, the aim of the experiments published by Juzo ń et al [85] was to determine how two different 2,4-D concentrations affected the conversion of embryos into haploid plants and the subsequent development of fertile DH lines.…”

Section: Induction Of Haploid Embryosmentioning

confidence: 99%

Oat (Avena sativa L.) In Vitro Cultures: Prospects and Challenges for Breeding

Warchoł,

Skrzypek,

Juzoń-Sikora

et al. 2023

Agronomy

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Plant in vitro cultures have been a crucial component of efforts to enhance crops and advance plant biotechnology. Traditional plant breeding is a time-consuming process that, depending on the crop, might take up to 25 years before an improved cultivar is available to farmers. This is a problematic technique since both beneficial qualities (such as pest resistance) and negative ones (such as decreased yield) can be passed down from generation to generation. In vitro cultures provide various advantages over traditional methods, including the capacity to add desirable characteristics and speed up the development of new cultivars. When it comes to oat (Avena sativa L.), the efficient method of plant regeneration is still missing compared to the most common cereals, possibly because this cereal is known to be recalcitrant to in vitro culture. In this review, an effort has been made to provide a succinct overview of the various in vitro techniques utilized or potentially involved in the breeding of oat. The present work aims to summarize the crucial methods of A. sativa L. cultivation under tissue culture conditions with a focus on the progress that has been made in biotechnological techniques that are used in the breeding of this species.

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“…Toxic influence of 2,4-D provoke disturbances in bilayer phospholipid structure that plays an important role in the proper function of cell membrane. 6,7 Among endocrine disrupting compounds (EDCs) 2,4-D are of great environmental concern because of their high solubility in water and toxicity to humans, animals and aquatic life. 8 The World Health Organization has recommended 70 mg L À1 as its maximum permissible concentration of 2,4-D in drinking water.…”

Section: Introductionmentioning

confidence: 99%