Synthesis, characterization &amp; evaluation of venom neutralization potential of silver nanoparticles mediated Alstonia scholaris Linn bark extract

Ghosh, Rituparna; Sarkhel, Sumana; Saha, Kanchan; Parua, Poulami; Chatterjee, Upasana; Mana, Koushik

doi:10.1016/j.toxrep.2021.04.006

Cited by 8 publications

(4 citation statements)

References 34 publications

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“…There have been a few reports on the formulation of nanoparticles to reduce the toxic effects of snake venom proteins. Silver nanoparticles prepared in the presence of Alstonia scholaris Linn bark extract effectively neutralized Viper russelli venom ( Ghosh et al, 2021 ). Another report states that 2-hydroxy-4-methoxybenzoic acid (HMBA) from the root extract of the Indian Sarsaparilla ( Hemidesmus indicus ) has viper venom neutralizing effects in animal models ( Gomes et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of the Naja naja venom toxicity by polymeric nanoparticles loaded with Leucas aspera methanolic extract

Singh,

Jayaraman

2024

Front. Pharmacol.

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BackgroundSnakebite is a neglected tropical disease that affects millions of people worldwide. Developing effective treatments can make a significant contribution to global health efforts and public health initiatives. To reduce mortality due to snakebite, there is an immediate need to explore novel and effective treatment methodologies. In that context, nanoparticle-based drug delivery is gaining a lot of attention. Hydrophilic nanoparticles are suitable for the delivery of therapeutic peptides, proteins, and antigens.MethodsThe present investigation is aimed at evaluating the anti-ophidian potential of the methanolic extract of the ethno-medicinal herb Leucas aspera (Willd.) loaded within chitosan nanoparticles (CNP-LA), against the Indian cobra (Naja naja) venom enzymes. For this purpose, nanoparticles were prepared using the ionic gelation method to enhance the efficacy of the extract. The physicochemical and structural features of nanoparticles were investigated using dynamic light scattering (DLS), Fourier-transform Infrared (FTIR), field emission scanning electron microscopy (FE-SEM), and X-ray diffraction (XRD) techniques.ResultsIt was found that CNP-LA has an average size of 260 nm with a polydispersity index of 0.132 (PDI) and zeta potential of 34.7 mV, with an encapsulation efficiency of 92.46%. The in vitro release study was performed at pH 5.0 and 7.4. Furthermore, in vitro studies indicated that CNP-LA inhibited the phospholipase A2, hemolytic, and caseinolytic activities of Naja naja venom with the percentage inhibition of 92.5%, 83.9%, and 94.5%, respectively.ConclusionThis is the first report on the application of herbal methanolic extract loaded within chitosan nanoparticles for neutralizing snake venom enzymes with increased efficiency.

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Section: Discussionmentioning

confidence: 99%

Inhibition of the Naja naja venom toxicity by polymeric nanoparticles loaded with Leucas aspera methanolic extract

Singh,

Jayaraman

2024

Front. Pharmacol.

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“…Recently, with the rapid development in the uses of nanoparticles in the medical and industrial fields, many researchers have focused on devising new methods to reduce the toxicity of these materials [ [41] , [42] , [43] , [44] ]. In this study, we have examined the sexual behavior, body and weight of reproductive organs, spermatotoxicity, serum testosterone level, apoptosis in germ cells, testicular histopathology and antioxidant biomarkers in the testis of mice exposed to Ag-NPs and the protective effects of YO-NPs against Ag-NPs toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…It may be better to pre-treat the animals with YO-NPs instead of the treatment after Ag-NPs exposure [ 33 ]. However, the NPs effects differ depending on the synthesis method (chemical or biological synthesis) and coating agent used [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Alleviation of silver nanoparticle-induced sexual behavior and testicular parameters dysfunction in male mice by yttrium oxide nanoparticles

et al. 2021

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“…However, treatment with a low and high dose of MO-AgNPs significantly improved the haematological indices dosedependently and ameliorated the pathological defects noticed in the heart of envenomed treated rats. The observed improvement could be attributed to the neutralizing potentials of MO-AgNPs after treatment as studies have reported the neutralizing potency of silver nano particles-based plant extract against venoms of snake species (Singh et al 2020;Ghosh et al 2021).…”

Section: He ×400mentioning

confidence: 97%

Biosynthesis, characterization and antivenom activities of <i>Moringa oleifera</i> silver nanoparticles: an experimental approach

Adeyi,

Olowookorun,

Ajisebiola

et al. 2024

TZOOL

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Moringa oleifera has been previously established to possess neutralizing potentials against Echis ocellatus venom. This study however, investigated the bioefficacy of silver nanoparticles biosynthesized from M. oleifera leaf extract aimed at improving its bioactivity against E. ocellatus venom-induced toxicities using in vivo and in vitro methods. The intrinsic characteristics of the produced M. oleifera-Silver nanoparticles (MO-AgNPs) were carried out using energy dispersive X-ray, X-ray diffraction, scanning electron microscopy, transmission electron microscopy and Fourier-transform infrared spectroscopy. Twenty-five male Wistar rats divided randomly into five groups (n=5) were used for the antivenom study. Group 1 received saline while groups 2 to 5 were envenomed intraperitoneally with 0.22mg/kg (LD50) of E. ocellatus venom. Group 2 was left untreated while groups 3 to 5 were treated with 0.2ml of antivenom, 5 and 10mg/kg MO-AgNPs post-envenomation, respectively. Blood and tissue of treated rats were analyzed for heamatological parameters and histopathology, respectively. The MO-AgNPs formation was confirmed with a colour change from light brown to yellowish-brown with maximum SPR band at 420nm from UV-Vis analysis, indicating a reflection of the bio-reduction of Ag+ to Ag0. The Transmission electron micrographs showed well dispersed spherical AgNPs with average particle size of 15.7nm. Treatment with MO-AgNPs caused a significant improvement of acute anemia, leucopenia and thrombocytopenia induced by the venom in the envenomed treated rats. Also, MO-AgNPs inhibited the haemorrhagic, haemolytic and anticoagulant activities of the venom. Tissue lesions observed in heart of envenomed untreated rats were attenuated after treatment with MO-AgNPs. The biosynthesized MO-AgNPs exhibited potent neutralizing potentials than M. oleifera crude extract against E. ocellatus venom toxicities.

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Synthesis, characterization & evaluation of venom neutralization potential of silver nanoparticles mediated Alstonia scholaris Linn bark extract

Abstract: Graphical abstract

Cited by 8 publications

References 34 publications

Inhibition of the Naja naja venom toxicity by polymeric nanoparticles loaded with Leucas aspera methanolic extract

Inhibition of the Naja naja venom toxicity by polymeric nanoparticles loaded with Leucas aspera methanolic extract

Alleviation of silver nanoparticle-induced sexual behavior and testicular parameters dysfunction in male mice by yttrium oxide nanoparticles

Biosynthesis, characterization and antivenom activities of <i>Moringa oleifera</i> silver nanoparticles: an experimental approach

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