PpEBB1 directly binds to the GCC box-like element of auxin biosynthesis related genes

Zhao, Xuehui; Wen, Binbin; Li, Chen; Liu, Li; Chen, Xiude; Li, Dongmei; Li, Ling; Fu, Xiling

doi:10.1016/j.plantsci.2021.110874

Cited by 3 publications

(3 citation statements)

References 41 publications

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“…In addition, the AP2 ERF domain binds to a GCC box (Chandler, 2018). As shown in a previous study, PpEBB1 could directly bind to the GCC box-like element of auxin biosynthesis-related genes including STY1, SRS5, and YUC1, and promote their expression (Zhao et al, 2021). Therefore, some of the DEGs involved in these processes may directly be regulated by PpEBB1 but needs to be further studied.…”

Section: Discussionmentioning

confidence: 87%

Overexpression of the Peach Transcription Factor Early Bud-Break 1 Leads to More Branches in Poplar

Zhao

Wen

et al. 2021

Front. Plant Sci.

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Shoot branching is an important adaptive trait that determines plant architecture. In a previous study, the Early bud-break 1 (EBB1) gene in peach (Prunus persica var. nectarina) cultivar Zhongyou 4 was transformed into poplar (Populus trichocarpa). PpEBB1-oe poplar showed a more branched phenotype. To understand the potential mechanisms underlying the EBB1-mediated branching, transcriptomic and proteomics analyses were used. The results showed that a large number of differentially expressed genes (DEGs)/differentially expressed proteins (DEPs) associated with light response, sugars, brassinosteroids (BR), and nitrogen metabolism were significantly enriched in PpEBB1-oe poplar. In addition, contents of sugars, BR, and amino acids were measured. Results showed that PpEBB1 significantly promoted the accumulation of fructose, glucose, sucrose, trehalose, and starch. Contents of brassinolide (BL), castasterone (CS), and 6-deoxocathasterone (6-deoxoCS) were all significantly changed with overexpressing PpEBB1. Various types of amino acids were measured and four of them were significantly improved in PpEBB1-oe poplar, including aspartic acid (Asp), arginine (Arg), cysteine (Cys), and tryptohpan (Trp). Taken together, shoot branching is a process controlled by a complex regulatory network, and PpEBB1 may play important roles in this process through the coordinating multiple metabolic pathways involved in shoot branching, including light response, phytohormones, sugars, and nitrogen.

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Section: Discussionmentioning

confidence: 87%

Overexpression of the Peach Transcription Factor Early Bud-Break 1 Leads to More Branches in Poplar

Zhao

Wen

et al. 2021

Front. Plant Sci.

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“…Yeast one-hybrid (Y1H) assays were performed as previously described (Zhao et al 2021 ). Briefly, the MdNAC4 CDS was fused to the pGADT7 vector.…”

Section: Methodsmentioning

confidence: 99%

The NAC transcription factor MdNAC4 positively regulates nitrogen deficiency-induced leaf senescence by enhancing ABA biosynthesis in apple

et al. 2023

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Although it is well established that nitrogen (N) deficiency induces leaf senescence, the molecular mechanism of N deficiency-induced leaf senescence remains largely unknown. Here, we show that an abscisic acid (ABA)-responsive NAC transcription factor (TF) is involved in N deficiency-induced leaf senescence. The overexpression of MdNAC4 led to increased ABA levels in apple calli by directly activating the transcription of the ABA biosynthesis gene MdNCED2. In addition, MdNAC4 overexpression promoted N deficiency-induced leaf senescence. Further investigation showed that MdNAC4 directly bound the promoter of the senescence-associated gene (SAG) MdSAG39 and upregulated its expression. Interestingly, the function of MdNAC4 in promoting N deficiency-induced leaf senescence was enhanced in the presence of ABA. Furthermore, we identified an interaction between the ABA receptor protein MdPYL4 and the MdNAC4 protein. Moreover, MdPYL4 showed a function similar to that of MdNAC4 in ABA-mediated N deficiency-induced leaf senescence. These findings suggest that ABA plays a central role in N deficiency-induced leaf senescence and that MdPYL4 interacts with MdNAC4 to enhance the response of the latter to N deficiency, thus promoting N deficiency-induced leaf senescence. In conclusion, our results provide new insight into how MdNAC4 regulates N deficiency-induced leaf senescence. Graphical Abstract

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“…com/article/10.3390/ijms24108514/s1. References [115,116,[119][120][121][122][123][124][125] are cited in Supplementary Materials. Institutional Review Board Statement: Not applicable.…”

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confidence: 99%

Precise Regulation of the TAA1/TAR-YUCCA Auxin Biosynthesis Pathway in Plants

Luo

2023

IJMS

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The indole-3-pyruvic acid (IPA) pathway is the main auxin biosynthesis pathway in the plant kingdom. Local control of auxin biosynthesis through this pathway regulates plant growth and development and the responses to biotic and abiotic stresses. During the past decades, genetic, physiological, biochemical, and molecular studies have greatly advanced our understanding of tryptophan-dependent auxin biosynthesis. The IPA pathway includes two steps: Trp is converted to IPA by TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS/TRYPTOPHAN AMINOTRANSFERASE RELATED PROTEINs (TAA1/TARs), and then IPA is converted to IAA by the flavin monooxygenases (YUCCAs). The IPA pathway is regulated at multiple levels, including transcriptional and post-transcriptional regulation, protein modification, and feedback regulation, resulting in changes in gene transcription, enzyme activity and protein localization. Ongoing research indicates that tissue-specific DNA methylation and miRNA-directed regulation of transcription factors may also play key roles in the precise regulation of IPA-dependent auxin biosynthesis in plants. This review will mainly summarize the regulatory mechanisms of the IPA pathway and address the many unresolved questions regarding this auxin biosynthesis pathway in plants.

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PpEBB1 directly binds to the GCC box-like element of auxin biosynthesis related genes

Cited by 3 publications

References 41 publications

Overexpression of the Peach Transcription Factor Early Bud-Break 1 Leads to More Branches in Poplar

Overexpression of the Peach Transcription Factor Early Bud-Break 1 Leads to More Branches in Poplar

The NAC transcription factor MdNAC4 positively regulates nitrogen deficiency-induced leaf senescence by enhancing ABA biosynthesis in apple

Precise Regulation of the TAA1/TAR-YUCCA Auxin Biosynthesis Pathway in Plants

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