Proteomic profile of extracellular vesicles in anaphylaxis and their role in vascular permeability

Abstract

To the Editor, Anaphylaxis is a life-threatening allergic disorder 1 for which there is a need for improved diagnostic techniques and a deeper understanding of the molecular mechanisms involved. 2 Extracellular vesicles (EVs) play a key role in cellular communication, offering new possibilities with which to analyze patient particularities. 3 We hypothesized that anaphylaxis-derived EVs could provide potential markers of anaphylaxis due to their participation in the underlying molecular mechanisms. Vesicles were purified from 86 plasma samples (collected from 43 patients) during the acute phase of anaphylaxis (AnEVs) and baseline (BEVs). Clinical description, demographic characteristics, and their experimental utilities are displayed in Supplementary Table S1.AnEVs and BEVs were isolated and characterized. Electron microscopy and nanoparticle tracking analysis (NTA) showed particles that were consistent with the reported size range for EVsThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Increased miR‐21‐3p and miR‐487b‐3p serum levels during anaphylactic reaction in food allergic children

“…Due to a recent study from our group that demonstrated an active participation of extracellular vesicles interacting with ECs in anaphylaxis, 16 an in vitro serum‐EC system was carried out to evaluate the functional role of miR‐21‐3p and miR‐487b‐3p in this event. In order to do so, intracellular levels of both miRNAs were measured after incubation with anaphylactic conditions: cocktail of mediators and serum from patients (acute and basal phases).…”

“…Human dermal microvascular ECs (HMVEC‐D) were acquired from Lonza CC‐2543 and maintained in EGM™‐2MV BulletKit™ as previously described. 16 , 17 EC monolayers were incubated for 2 hours with: EGM‐2 medium (negative control), a cocktail of anaphylaxis mediators, or serum samples (acute and basal) from 5 patients. Mediators included in the cocktail were histamine, platelet‐activating factor (PAF), and thrombin (all from Sigma).…”

A systematic review and meta-analysis of proteomic and metabolomic alterations in anaphylaxis reactions

“…Two studies assessed the proteome change in anaphylaxis in patients ( 24 , 25 ). In the first study, the authors aimed to pinpoint specific biomarkers in plasma for predicting individuals with mastocytosis at a heightened risk of experiencing anaphylaxis.…”

Pathophysiological, Cellular, and Molecular Events of the Vascular System in Anaphylaxis

“…The fibroblast growth factor-inducible 14 (Fn14) receptor, the signal transducer and activator of transcription 3 (STAT3), the peroxisome proliferator-activated receptor (PPAR β/δ), and MALT1 protease activity have been proposed as therapeutic strategies in allergy and anaphylaxis ( 84 , 175 , 183 – 185 ). In addition, recently, a challenge study has demonstrated the capacity of EVs purified from plasma of anaphylactic patients to interact with ECs, eliciting vascular permeability and thereby suggesting that cellular communication between microenvironments may also be established in anaphylaxis ( 186 ).…”