Increased miR‐21‐3p and miR‐487b‐3p serum levels during anaphylactic reaction in food allergic children

Nuñez‐Borque, Emilio; Fernández-Bravo, Sergio; Río, Pablo Rodríguez del; Alwashali, Ebrahim; López-Domínguez, David; Gutierrez‐Blazquez, Maria Dolores; Laguna, José Julio; Tomé-Amat, Jaime; Gállego-Delgado, Julio; Gómez-López, Alicia; Betancor, Diana; Cuesta‐Herranz, Javier; Ibáñez-Sandín, María Dolores; Benito-Martín, Alberto; Esteban, Vanesa

doi:10.1111/pai.13518

Cited by 17 publications

(23 citation statements)

References 44 publications

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“…In human samples, the analysis of miRNA levels demonstrated variations in these molecules during this pathological event. Specifically, a study in a pediatric population with foodinduced anaphylaxis has shown increased circulating serum levels of miR-21-3p and miR-487b-3p [181]. Similarly, other evidences propose miR-451a as the most relevant biomarker in adult blood samples [88].…”

Section: Micrornasmentioning

confidence: 93%

Anaphylaxis: Mediators, Biomarkers, and Microenvironments

Fernández-Bravo¹,

Palacio-Garcia²,

Requena-Robledo³

et al. 2022

J Investig Allergol Clin Immunol

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The life-threating nature of the anaphylactic reactions promotes a growing interest to discover new biomarkers that could support their better diagnosis and prevention. However, both the clinical features and the etiopathology of anaphylaxis are very diverse, hindering the elucidation of valuable molecular indicators of disease. Most studies of anaphylaxis have focused on the immune system. Precisely, anaphylactic reactions are characterized primarily by IgE-mediated activation of mast cells and basophils and the release of several mediators. Among them, determinations of the serum tryptase levels is the main in vitro test used to confirm the reaction and there are no available biomarkers with predictive capacity for this pathological event. However, recent research has postulated that alternative pathways, cell types and systems are involved. Consequently, different molecular products have been explored and indicated as potential biomarkers, but none of them have been translated into the clinical practice yet. Precisely, vasoactive agents, proteases, proteoglycans, lipids, interleukins, cytokines, products of the complement-contact and coagulation systems, circulating proteins, extracellular vesicles, microRNAs and metabolites have been found to be altered in patients with anaphylaxis. The recognition of biological processes and molecular pathways interacting in the magnitude of the microenvironments switched on in anaphylaxis will notably nourish the clinical practice and the recognition of better molecular markers. Therefore, this article covers a broad review of the different mediators described in anaphylaxis and their proposal as biomarkers of this pathological event, as well as their role in the molecular basis of the reaction.

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Section: Micrornasmentioning

confidence: 93%

Anaphylaxis: Mediators, Biomarkers, and Microenvironments

Fernández-Bravo¹,

Palacio-Garcia²,

Requena-Robledo³

et al. 2022

J Investig Allergol Clin Immunol

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“…Mechanistically, when the individual is first exposed to an allergen, the immune sensitization process is initiated, triggering the production of antigen-specific IgE antibodies. In successive reexposures to the antigen, there is cross-linking of FcϵRI-bound allergen-IgE complexes, activating effector cells and giving rise to the release of a large number of mediators ( 12 , 13 , 30 ) ( Figure 1 ).…”

Section: Immune System In Anaphylaxismentioning

confidence: 99%

“…Several triggers can induce anaphylaxis, the most common being foods, drugs, and Hymenoptera venoms, although in some cases the cause of the reaction is unknown (idiopathic) (12)(13)(14). In particular, the etiological distribution of anaphylaxis differs with age since the most common triggers are drugs and insect stings in adults, while food is the main culprit in children (5,15).…”

Section: Anaphylaxismentioning

confidence: 99%

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Pathophysiological, Cellular, and Molecular Events of the Vascular System in Anaphylaxis

et al. 2022

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Anaphylaxis is a systemic hypersensitivity reaction that can be life threatening. Mechanistically, it results from the immune activation and release of a variety of mediators that give rise to the signs and symptoms of this pathological event. For years, most of the research in anaphylaxis has focused on the contribution of the immune component. However, approaches that shed light on the participation of other cellular and molecular agents are necessary. Among them, the vascular niche receives the various signals (e.g., histamine) that elicit the range of anaphylactic events. Cardiovascular manifestations such as increased vascular permeability, vasodilation, hypotension, vasoconstriction, and cardiac alterations are crucial in the pathophysiology of anaphylaxis and are highly involved to the development of the most severe cases. Specifically, the endothelium, vascular smooth muscle cells, and their molecular signaling outcomes play an essential role downstream of the immune reaction. Therefore, in this review, we synthesized the vascular changes observed during anaphylaxis as well as its cellular and molecular components. As the risk of anaphylaxis exists both in clinical procedures and in routine life, increasing our knowledge of the vascular physiology and their molecular mechanism will enable us to improve the clinical management and how to treat or prevent anaphylaxis.Key MessageAnaphylaxis, the most severe allergic reaction, involves a variety of immune and non-immune molecular signals that give rise to its pathophysiological manifestations. Importantly, the vascular system is engaged in processes relevant to anaphylactic events such as increased vascular permeability, vasodilation, hypotension, vasoconstriction, and decreased cardiac output. The novelty of this review focuses on the fact that new studies will greatly improve the understanding of anaphylaxis when viewed from a vascular molecular angle and specifically from the endothelium. This knowledge will improve therapeutic options to treat or prevent anaphylaxis.

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“…Two different molecules (Regulator of calcineurin 1 and Fibroblast growth factorinducible molecule 14) may be involved in the modulation of histamine-induced endothelial barrier disfunction in anaphylaxis, as revealed in cellular and murine experiments, but their role as biomarkers is not established [102,103]. Because endothelial cells increase the miR-21-3p intracellularly and release it in response to anaphylaxis, this researched microRNA may be a future candidate biomarker [104].…”

Section: Other Potential Biomarkers For Diagnosis Of Anaphylaxis In the Acute Care Settingmentioning

confidence: 99%

State-of-the-Art on Biomarkers for Anaphylaxis in Obstetrics

Simionescu

Stănescu

Popescu

2021

Life

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Anaphylaxis is an unpredictable systemic hypersensitivity reaction and constitutes a high risk of maternal and fetal morbidity and mortality when occurring during pregnancy. Currently, the acute management of anaphylaxis is based on clinical parameters. A total serum tryptase is only used to support an accurate diagnosis. There is a need to detect other biomarkers to further assess high-risk patients in obstetrics. Our objective is to present biomarkers in this complex interdisciplinary approach beyond obstetrician and anaesthetic management. Candidate biomarkers derive either from mediators involved in immunopathogenesis or upcoming molecules from systems biology and proteomics. Serum tryptase is determined by singleplex immunoassay method and is important in the evaluation of anaphylactic mast cell degranulation but also in the assessment of other risk factors for anaphylaxis such as systemic mastocytosis. Another category of biomarkers investigates the IgE-mediated sensitization to triggers potentially involved in the etiology of anaphylaxis in pregnant women, using singleplex or multiplex immunoassays. These in vitro tests with natural extracts from foods, venoms, latex or drugs, as well as with molecular allergen components, are useful because in vivo allergy tests cannot be performed on pregnant women in such a major medical emergency due to their additional potential risk of anaphylaxis.

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Increased miR‐21‐3p and miR‐487b‐3p serum levels during anaphylactic reaction in food allergic children

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 17 publications

References 44 publications

Anaphylaxis: Mediators, Biomarkers, and Microenvironments

Anaphylaxis: Mediators, Biomarkers, and Microenvironments

Pathophysiological, Cellular, and Molecular Events of the Vascular System in Anaphylaxis

State-of-the-Art on Biomarkers for Anaphylaxis in Obstetrics

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