Pharmacological importance of TG12 from tachykinin and its toxicological behavior against multidrug-resistant bacteria Klebsiella pneumonia

Raju, Stefi V.; Sarkar, Purabi; Pasupuleti, Mukesh; Saraswathi, Nadar; Arasu, Mariadhas Valan; Al-Dhabi, Naïf Abdullah; Esmail, Galal Ali; Arshad, Aziz; Arockiaraj, Jesu

doi:10.1016/j.cbpc.2021.108974

Cited by 6 publications

(1 citation statement)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A full-length tachykinin protein was identified from the earlier established muscle transcriptome of a freshwater teleost C. striatus (Raju et al 2021). While analyzing the tachykinin protein using bioinformatics tools (antimicrobial prediction algorithm), a short peptide molecule named RM12 was identified, which is similar to peptide, substance P. The biological properties of the identified peptide, RM12, were analyzed using various bioinformatic programs including multiple sequence alignment and structural analysis.…”

Section: Methodsmentioning

confidence: 99%

RM12 similar to substance P from tachykinin of freshwater murrel Channa striatus influence intracellular ROS in vitro fish erythrocytes and developmental toxicity and antioxidant enzymes in vivo zebrafish embryo

Raju

Mukherjee

Sarkar

et al. 2021

Fish Physiol Biochem

Self Cite

View full text Add to dashboard Cite

zebrafish embryo, the intracellular ROS level was estimated by 5 mM H 2 O 2 stress in the zebrafish embryo, and inhibition of apoptosis was evaluated. The antioxidant enzymes were extracted from the H 2 O 2 -stressed zebrafish embryo, and the intracellular ROS was eliminated due to RM12. Collectively, the experiment showed that the substance P from the freshwater murrel C. striatus possessed potent antioxidant properties; thus, it can further be focused to develop it as antioxidant molecule in aquaculture organisms. KeywordsSubstance P • Tachykinin • Channa striatus • Antioxidant mechanism • Intracellular ROS Abbreviations ROS Reactive oxygen species H 2 O 2 Hydrogen peroxide ESR Electron spin resonance DPPH 2,2-Diphenyl-1-picryl-hydrazyl-hydrate ABTS 2,2-Azinobis 3-ethylbenzothiazoline-6-sulfonic acid SOAH Superoxide anion radical scavenging assay HORS Hydroxyl radical scavenging assay DCFH-DA 2′-7′-Dichlorodihydrofluorescein diacetate TEAC Trolox equivalent antioxidant capacity PMS Phenazine methosulphate NADH Nicotinamide adenine dinucleotide NBT Nitro blue tetrazolium

show abstract

Section: Methodsmentioning

confidence: 99%