Lophatherum gracile Brongn. attenuates neutrophilic inflammation through inhibition of JNK and calcium

Lai, Kuei Hung; Chen, Po‐Jen; Chen, Chih Chuan; Yang, Shun-Min; El-Shazly, Mohamed; Chang, Yu Chia; Wu, Yi; Wu, Yuan-Hua; Wang, Yi‐Hsuan; Hsieh, Hsi‐Lung; Hwang, Tsong Long

doi:10.1016/j.jep.2020.113224

Cited by 26 publications

(25 citation statements)

References 42 publications

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“…Blood was acquired from human donors (20~30 years old) by venipuncture under the approval and supervision of the Institutional Review Board (IRB) at Chang Gung Memorial Hospital. Neutrophils were purified utilizing a protocol of dextran sedimentation, hypotonic lysis, and Ficoll Hypaque gradient of erythrocytes according to previous reported methods [22]. Isolated human neutrophils were suspended in a calcium (Ca 2+ )-free HBSS buffer at pH 7.4 and examined by the trypan blue exclusion method (>98% viable cells).…”

Section: Preparation Of Human Neutrophilsmentioning

confidence: 99%

“…•− generation was assessed using superoxidase dismutase (SOD) inhibitable reduction of ferricytochrome c [22]. After supplementation with ferricytochrome c (0.6 mg/mL), neutrophils (6 × 10 5 cells/mL) were equilibrated at 37 • C and incubated for 5 min before treatment with pure compounds or DMSO (0.1%, control).…”

Section: Measurement Of Superoxide Anion (Omentioning

confidence: 99%

“…•− generation) and degranulation (elastase release) [22]. In order to evaluate the anti-inflammatory activities of scalaranes, all isolates (except for 5 owing to an amount limitation) were assayed in fMLF-induced human neutrophils (Table 5).…”

Section: Assessments Of O 2 •− Generation and Elastase Release In Fmlf-activated Human Neutrophilsmentioning

confidence: 99%

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Scalarane-Type Sesterterpenoids from the Marine Sponge Lendenfeldia sp. Alleviate Inflammation in Human Neutrophils

et al. 2021

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Sponge-derived scalaranes are remarkable sesterterpenoids previously found to exhibit profound inhibitory effects against neutrophilic inflammation. In our current work, we constructed the metabolomic profile of marine sponge Lendenfeldia sp. for the first time using a tandem mass spectrometry (MS/MS) molecular networking approach. The results highlighted the rich chemical diversity of these scalaranes, motivating us to conduct further research to discover novel scalaranes targeting neutrophilic inflammation. MS- and NMR-assisted isolation and elucidation led to the discovery of seven new homoscalaranes, lendenfeldaranes K–Q (1–7), characterized by methylation at C-24, together with five known derivatives, lendenfeldarane B (8), 25-nor-24-methyl-12,24-dioxoscalar-16-en-22-oic acid (9), 24-methyl-12,24,25-trioxoscalar-16-en-22-oic acid (10), felixin B (11), and 23-hydroxy-20-methyldeoxoscalarin (12). Scalaranes 1–4 and 6–12 were assayed against superoxide anion generation and elastase release, which represented the neutrophilic inflammatory responses of respiratory burst and degranulation, respectively. The results indicated that 1–3 and 6–12 exhibited potential anti-inflammatory activities (IC50 for superoxide anion scavenging: 0.87~6.57 μM; IC50 for elastase release: 1.12~6.97 μM).

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Section: Preparation Of Human Neutrophilsmentioning

confidence: 99%

Section: Measurement Of Superoxide Anion (Omentioning

confidence: 99%

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Scalarane-Type Sesterterpenoids from the Marine Sponge Lendenfeldia sp. Alleviate Inflammation in Human Neutrophils

et al. 2021

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“…Phenolic compounds are the main active components in L. gracile, with the representative phenolic compounds being the phenolic acids (e.g., 3,4-dihydroxybenzoic acid, neochlorogenic acid, syringic acid, chlorogenic acid, and p-coumaric acid) and the flavonoids (e.g., orientin, isoorientin, and swertiajaponin) [1][2][3]. These components possess strong biological activities, such as antibiosis, antitumor, anti-inflammatory, antiviral, hepatoprotective, and antioxidant activities [4][5][6][7]. For example, chlorogenic acid and its derivatives are effective dietary protective phenolic compounds, and are negatively correlated with various harmful conditions, such as oxidative stress, inflammatory stress, metabolic syndrome, hepatic steatosis, acute lung injury, neurodegenerative diseases, mental disorders, cardiovascular diseases, gastrointestinal dysfunction, and cancer [8].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the phytochemicals and antioxidant activity of Lophatherum gracile Brongn based on chemical fingerprinting by HPLC with electrochemical detection

Zhang

Chun

Shao

et al. 2021

J of Separation Science

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A combinative method using high-performance liquid chromatographyelectrochemical detection for fingerprinting and quantitative analysis was developed and successfully applied for the quality evaluation of Lophatherum gracile Brongn leaves collected from 21 geographical locations in China. In the fingerprint analysis, 18 common peaks were observed among the 21 samples, and 10 peaks were identified. Simultaneous quantification of the 10 components was conducted to interpret the variations in these compounds among the L. gracile Brongn leaves originating from different geographical locations. The correlation between the chromatograms and the antioxidant activities of the samples was further studied. The results indicated a linear correlation between the antioxidant activity and the total common peak areas of the fingerprints obtained by high-performance liquid chromatographyelectrochemical detection. Importantly, it was found that high-performance liquid chromatography-electrochemical detection fingerprinting can not only determine the quantities of individual components present in such samples but also evaluate the antioxidant activities of the samples. The developed method is a valuable reference for the further study and development of L. gracile Brongn.

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“…Lophatherum gracile belongs to Gramineae family and is a perennial herb. Its dried stems and leaves can be used for medicine to clinically clear heat, disinhibit dampness, and treat inflammation (Editorial Committee of flora of China 2002; Y. Chen 2014; Wang et al 2016;Lai et al 2021). Its leaves are rich in flavonoids, phenolic acids and polysaccharides (Xue et al 2009;Zhang 2010;Tang et al 2012;).…”

mentioning

confidence: 99%

Chloroplast genome structure and phylogenetic position of Lophatherum gracile

Zhou

Dai

et al. 2021

Mitochondrial DNA Part B

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Lophatherum gracile is distributed in south China, Japan and South Asia, and it is wild in the valley, stream, woodland, forest edge and gully edge. In this study, the complete chloroplast genome sequence of Lophatherum gracile was successfully obtained using Illumina sequencing. The full length of the chloroplast genome length was 137,749 bp with a typical quadripartite structure: one large single copy (LSC) region (80,610 bp), one small single copy (SSC) region (12,429 bp), and a pair of inverted repeats (IRs) (22,355 bp each). The GC content of this genome was 38.64%. The whole genome contained 130 genes, including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis indicated that Lophatherum gracile was closely related to Cenchrus americanus and Cenchrus longispinus.

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Lophatherum gracile Brongn. attenuates neutrophilic inflammation through inhibition of JNK and calcium

Cited by 26 publications

References 42 publications

Scalarane-Type Sesterterpenoids from the Marine Sponge Lendenfeldia sp. Alleviate Inflammation in Human Neutrophils

Scalarane-Type Sesterterpenoids from the Marine Sponge Lendenfeldia sp. Alleviate Inflammation in Human Neutrophils

Evaluation of the phytochemicals and antioxidant activity of Lophatherum gracile Brongn based on chemical fingerprinting by HPLC with electrochemical detection

Chloroplast genome structure and phylogenetic position of Lophatherum gracile

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