Scalarane-Type Sesterterpenoids from the Marine Sponge Lendenfeldia sp. Alleviate Inflammation in Human Neutrophils

Peng, Bo; Lai, Kuei Hung; Lee, Gene Hsiang; Yu, Steve S.‐F.; Duh, Chang‐Yih; Su, Jui Hsin; Zheng, Li Guo; Hwang, Tsong‐Long; Sung, Ping‐Jyun

doi:10.3390/md19100561

Cited by 10 publications

(24 citation statements)

References 26 publications

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“…The superoxidase dismutase (SOD) inhibitable reduction of ferricytochrome c was used to determine O 2 •− generation [ 30 ]. Neutrophils (6 × 10 5 cells/mL) were supplemented with ferricytochrome c (0.6 mg/mL), were equilibrated at 37 °C, and were incubated for 5 min before treatment with the LJEE extract (3, 10, and 30 μg/mL), pure compounds (3, 10, and 20 μM), or DMSO (0.1%, as the control).…”

Section: Methodsmentioning

confidence: 99%

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Lonicerae Japonicae Flos Attenuates Neutrophilic Inflammation by Inhibiting Oxidative Stress

et al. 2022

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Lonicerae japonicae flos (LJ) is an Asian traditional herb that is used as a dietary supplement, tea, and beverage to clear heat and quench thirst. However, no studies investigated its effect on activated human neutrophils, which played a crucial role in the bad prognosis of coronavirus disease of 2019 (COVID-19) patients by aggravating lung inflammation and respiratory failure. Herein, we evaluated the anti-inflammatory effect of LJ ethanol extract (LJEE) on human neutrophils activated by N-formyl-methionyl-leucyl-phenylalanine (fMLF). Our experimental results indicated that LJEE suppressed fMLF-activated superoxide anion (O2•−) generation, the expression of CD11b, and cell adhesion and migration, as well as the formation of neutrophil extracellular traps in human neutrophils. Further in-depth mechanical investigation revealed that pretreatment with LJEE accelerated the Ca2+ clearance, but did not affect the phosphorylation of mitogen-activated protein kinases (MAPKs) and protein kinase B (Akt) in activated human neutrophils. In addition, LJEE displayed a dose-dependent reactive oxygen species (ROS) scavenger activity, which assisted its anti-inflammatory activity. From the bioassay-coupled chromatographic profile, chlorogenic acids were found to dominate the anti-inflammatory effects of LJEE. Moreover, LJ water extract (LJWE) demonstrated an interrupting effect on the severe acute respiratory syndrome coronavirus-2 spike protein (SARS-CoV-2-Spike)/angiotensin-converting enzyme 2 (ACE2) binding. In conclusion, the obtained results not only supported the traditional use of LJ for heat-clearance, but also suggested its potential application in daily health care during the COVID-19 pandemic.

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Section: Methodsmentioning

confidence: 99%

“…In 100 μM of 99% ethanol, DPPH was dissolved and was incubated with vitamin E (α-tocopherol, the positive control; 1, 3, 5, and 10 μg/mL) or LJEE extract (1, 3, 10, and 30 μg/mL) at 25 °C for 15 min [ 30 ]. A spectrophotometer (U-3010, Hitachi, Tokyo, Japan), was used to measure the absorbance variations at 517 nm (in 4.5 mL cuvette).…”

Section: Methodsmentioning

confidence: 99%

Lonicerae Japonicae Flos Attenuates Neutrophilic Inflammation by Inhibiting Oxidative Stress

et al. 2022

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“…Both metabolites exhibited weaker cytotoxicity against MIA-paca and PANC-1 cell lines with IC 50 values of 31.1 and 34.3, and 45.9 and 38.8 μM, respectively. [25] An unidentified sponge derived from the Madagascar, produced 12α-hydroxy-22-hydroxy-24-methyl-24oxoscalar-16-en-25α-al (30) and 12β-hydroxy-22hydroxy-24-methyl-24-oxoscalar-16-en-25β-al (31). Both compounds displayed strong antiproliferative activities towards the A2780 with IC 50 values of 0.28 and 0.26 μM, respectively.…”

Section: Tetracyclic Scalaranesmentioning

confidence: 99%

Bioactive Scalarane‐Type Sesterterpenoids from Marine Sources

Chen

Duan

et al. 2022

Chemistry & Biodiversity

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Scalarane‐type sesterterpenoids have received considerable attention in the scientific literature due to their diverse carbon skeletons and various biological activities and pharmacological properties. Among all these derivatives are commonly isolated from marine sponges and are occasionally derived from shell‐less mollusks, such as nudibranchs. This review comprehensively discusses the marine‐derived natural sources that give rise to these scalarane‐type sesterterpenoids, providing the names, their chemical structures, biological properties, with emphasis on anticancer activity and literature references related to these metabolites. A critical summary of the 221 compounds generated from January 2010 up to December 2021 for their potential as anticancer agents is presented.

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“…This finding suggested the potential of 2 as well as other scalarane-type sesterterpenoids to serve as natural products to manage clinical manifestations caused by the overexpression of CXCL10, such as tumorous diseases (Liu et al, 2011b), autoimmune diseases (Lee et al, 2009), and infectious diseases (Liu et al, 2011a). These scalarane-type sesterpenoids have drawn much attention due to the recent findings as potent anti-inflammatory agents (Peng et al, 2020a;Chakraborty and Francis, 2021;Peng et al, 2021). However, research has been carried out herein to illustrate for the first time the antineurofibroma potential of these sponge-derived scalaranes.…”

Section: Mimn-guided Isolation and Validation Of Anti-neurofibroma Leadsmentioning

confidence: 99%

“…Among these techniques are the "MS/MS molecular networking (MN) approaches" (Watrous et al, 2012) that help in the secondary metabolites identification as well as the "spectroscopic coupled bioassay screening strategies" (Faleschini et al, 2019) that aid in the identification of the most potent natural components. These techniques drew most of the scientific community's attention and led to significant advances in bioactive metabolites discovery (Allard et al, 2016;Caesar et al, 2018;Woo et al, 2019;Olivon et al, 2020;Peng et al, 2021). MN is a visual computational strategy that can be intuitively implemented using LC-MS/MS data (Watrous et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Chemometric-Guided Exploration of Marine Anti-Neurofibroma Leads

et al. 2022

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In-depth analysis of metabolomics diversity of marine species through advanced mass spectrometric analysis is one of the most promising new tools for the development of marine drugs against mild and life-threatening diseases. Neurofibromas are a common type of tumor in the peripheral nervous system. Currently, there are very limited treatment options for neurofibromas. In our course of exploring potential therapeutic agents for neurofibroma treatment, the multi-informative molecular networking (MIMN) approach was proposed. The MIMNs of the Lendenfeldia sp. sponge extract and sub-fractions were established according to their inhibitory activity against several inflammatory chemokines (CCL3, CCL4, CCL5, CXCL1, CXCL8, and CXCL10) in neurofibroma cell line hTERT-NF1-ipNF95.11b-C (CRL-3390). The visualized MIMN revealed the anti-inflammatory potential of scalarane-enriched fractions, and the follow-up annotation and isolation led to the identification of a scalarane, 24-methyl-12,24,25-trioxoscalar-16-en-22-oic acid (2). Our results revealed that the most abundant scalarane (2) dominated the anti-chemokine effect of Lendenfeldia sp. extract together with other scalaranes, indicating the potential application of sponge-derived scalaranes to be developed as therapeutic agents for neurofibromas.

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Scalarane-Type Sesterterpenoids from the Marine Sponge Lendenfeldia sp. Alleviate Inflammation in Human Neutrophils

Cited by 10 publications

References 26 publications

Lonicerae Japonicae Flos Attenuates Neutrophilic Inflammation by Inhibiting Oxidative Stress

Lonicerae Japonicae Flos Attenuates Neutrophilic Inflammation by Inhibiting Oxidative Stress

Bioactive Scalarane‐Type Sesterterpenoids from Marine Sources

Chemometric-Guided Exploration of Marine Anti-Neurofibroma Leads

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