Chloroplast genome structure and phylogenetic position of Lophatherum gracile

Abstract

Lophatherum gracile is distributed in south China, Japan and South Asia, and it is wild in the valley, stream, woodland, forest edge and gully edge. In this study, the complete chloroplast genome sequence of Lophatherum gracile was successfully obtained using Illumina sequencing. The full length of the chloroplast genome length was 137,749 bp with a typical quadripartite structure: one large single copy (LSC) region (80,610 bp), one small single copy (SSC) region (12,429 bp), and a pair of inverted repeats (IRs) (22,355 bp each). The GC content of this genome was 38.64%. The whole genome contained 130 genes, including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis indicated that Lophatherum gracile was closely related to Cenchrus americanus and Cenchrus longispinus.

The complete chloroplast genome of Mussaenda pubescens and phylogenetic analysis

“…1 ) and stored immediately at − 80 °C. The isolation of total genomic DNA was performed using a modified CTAB method 22 . Library construction and sequencing were conducted by Novogene Bioinformatics Technology Co. Ltd. (Guangzhou, China) using the Illumina NovaSeq 6000 platform with a Paired-End 150 (PE150) strategy.…”

“…Library construction and sequencing were conducted by Novogene Bioinformatics Technology Co. Ltd. (Guangzhou, China) using the Illumina NovaSeq 6000 platform with a Paired-End 150 (PE150) strategy. The FastQC software was employed to trim low-quality reads and adapters, and the genome was de novo assembled using SPAdes v3.9 23 , and subsequently annotated using Plann software 22 . The accuracy of the preliminary annotation results was verified by comparing them with the proteins and rRNA sequences from previously reported cp genomes of relevant species using the methods of blastn and blastp.…”