Characterization of Cellulose Synthase A (CESA) Gene Family in Eudicots

Nawaz, Muhammad Amjad; Lin, Xiao; Chan, Ting‐Fung; Imtiaz, Muhammad; Rehman, Hafiz Mamoon; Ali, Muhammad Amjad; Baloch, Faheem Shehzad; Atif, Rana Muhammad; Yang, Seung Hwan; Chung, Gyuhwa

doi:10.1007/s10528-018-9888-z

Cited by 17 publications

(12 citation statements)

References 75 publications

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“…In this regard, the higher expression of GH10, GH44, and GH76, and increased diversity of enzymes belonging to gene families GH1, GH28, GH31, GH47, and GH88 clearly indicate that FSP has successfully established itself in common bean and a weakening response has started [17]. As most plant cell walls are based on a network of cellulose microfibrils and hemicelluloses [73], the upregulation of cellulose synthase-As, cellulose synthase-like enzymes, and other cell wall-strengthening enzymes in FS indicates that common bean cells reinforce cell wall polymers [74]. Callose, like cellulose, is another cell wall polymer that increases its strength, particularly during disease incidence.…”

Section: Cell Wall Acts As a Barrier Against Fsp Infection In Common Beanmentioning

confidence: 99%

Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.)

Chen¹,

Wu²,

He³

et al. 2020

Genes

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Common bean (Phaseolus vulgaris L.) is a major legume and is frequently attacked by fungal pathogens, including Fusarium solani f. sp. phaseoli (FSP), which cause Fusarium root rot. FSP substantially reduces common bean yields across the world, including China, but little is known about how common bean plants defend themselves against this fungal pathogen. In the current study, we combined next-generation RNA sequencing and metabolomics techniques to investigate the changes in gene expression and metabolomic processes in common bean infected with FSP. There were 29,722 differentially regulated genes and 300 differentially regulated metabolites between control and infected plants. The combined omics approach revealed that FSP is perceived by PAMP-triggered immunity and effector-triggered immunity. Infected seedlings showed that common bean responded by cell wall modification, ROS generation, and a synergistic hormone-driven defense response. Further analysis showed that FSP induced energy metabolism, nitrogen mobilization, accumulation of sugars, and arginine and proline metabolism. Importantly, metabolic pathways were most significantly enriched, which resulted in increased levels of metabolites that were involved in the plant defense response. A correspondence between the transcript pattern and metabolite profile was observed in the discussed pathways. The combined omics approach enhances our understanding of the less explored pathosystem and will provide clues for the development of common bean cultivars’ resistant to FSP.

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Section: Cell Wall Acts As a Barrier Against Fsp Infection In Common Beanmentioning

confidence: 99%

Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.)

Chen¹,

Wu²,

He³

et al. 2020

Genes

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“…The expression changes in CESAs suggests two things, i.e., first, cellulose synthesis in YS is affected similar to the lignin biosynthesis and, second, that YS24 may have lower cellulose deposition than SY24 (Nawaz et al, 2019). The second observation could not be generalized, since two other CESAs showed higher expression in YS24 than in CK1, and their expression pattern was similar in both pollination combinations.…”

Section: Discussionmentioning

confidence: 97%

“…The second observation could not be generalized, since two other CESAs showed higher expression in YS24 than in CK1, and their expression pattern was similar in both pollination combinations. The observation that there were three CESA transcripts could be due to the fact that there are multiple CESA members that differ in function and are expressed differently (Nawaz et al, 2017a(Nawaz et al, , 2019. Furthermore, in Arabidopsis, the silencing of CESA1 and CESA3 caused no pollen tube production (Persson et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Investigating the Mechanism of Unilateral Cross Incompatibility in Longan (Dimocarpus longan Lour.) Cultivars (Yiduo × Shixia)

Wang

Huang

et al. 2022

Front. Plant Sci.

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Longan (Dimocarpus longan Lour.) is an important subtropical fruit tree in China. Nearly 90% of longan fruit imports from Thailand are from the cultivar Yiduo. However, we have observed that there exists a unilateral cross incompatibility (UCI) when Yiduo is used as a female parent and Shixia (a famous Chinese cultivar) as a male parent. Here, we performed a comparative transcriptome analysis coupled with microscopy of pistils from two reciprocal pollination combinations [Shixia♂ × Yiduo♀(SY) and Yiduo♀ × Shixia♂(YS)] 4, 8, 12, and 24 h after pollination. We also explored endogenous jasmonic acid (JA) and jasmonyl isoleucine (JA-Ile) levels in pistils of the crosses. The microscopic observations showed that the UCI was sporophytic. The endogenous JA and JA-Ile levels were higher in YS than in SY at the studied time points. We found 7,251 differentially expressed genes from the transcriptome analysis. Our results highlighted that genes associated with JA biosynthesis and signaling, pollen tube growth, cell wall modification, starch and sucrose biosynthesis, and protein processing in endoplasmic reticulum pathways were differentially regulated between SY and YS. We discussed transcriptomic changes in the above-mentioned pathways regarding the observed microscopic and/or endogenous hormone levels. This is the first report on the elaboration of transcriptomic changes in longan reciprocal pollination combination showing UCI. The results presented here will enable the longan breeding community to better understand the mechanisms of UCI.

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“…Cellulose synthase A (CESA) superfamily genes, which include cellulose synthase (CESA) gene and cellulose synthase like gene (Csl), are related to mannan synthesis [12,13]. The first plant CesA gene was reported in cotton in 1996 [14].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of Cellulose-like synthase D gene family in Dendrobium catenatum

Liu

Chen

et al. 2021

Biotechnology & Biotechnological Equipment

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Dendrobium catenatum, which grows on the semi humid rocks in the mountains, has been at the top of the 'Nine Immortals of China' since ancient times. It is a kind of yin tonic medicine and its main active component is polysaccharide. Cellulose synthase-like D(CslD) genes were predicted to catalyse the biosynthesis of 1,4-β-d-glycan backbone of hemicelluloses, which plays fundamental roles in plant development. To investigate the role of CslD in the development of D. catenatum, eight CslD genes (DcCslD1,2a,2b,3a,3b,4a,4b,5) were identified. The results of protein prediction and analysis showed that CslD2a/2b/4a/4b proteins were acidic proteins. All the proteins had obvious hydrophobic or hydrophilic regions, and had transmembrane structure. Phylogenetic analysis revealed that the DcCslD family could be divided into group I, II, III and IV. DcCslD proteins had a typical Cellulose synthase domain and similar protein structures to the CslDs of other plants. Their promoter regions contain cis regulatory elements related to stress and hormone response. The results of qRT-PCR showed that the identified DcCslDs were differentially expressed in roots, stems and leaves. Most of them were highly expressed in stems and leaves. The environmental stresses examination showed that the expression levels of DcCslD5 were closely associated with drought-recovery treatment; the expression levels of DcCslD1, DcCslD2a, DcCslD2b, DcCslD3a and DcCslD5 were significantly influenced by low temperature. This study systematically analyzed the sequence characteristics of CslD protein of D. catenatum, which can provide reference for further study on the function of CslD protein in the polysaccharide metabolism of D. catenatum.

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Characterization of Cellulose Synthase A (CESA) Gene Family in Eudicots

Cited by 17 publications

References 75 publications

Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.)

Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.)

Investigating the Mechanism of Unilateral Cross Incompatibility in Longan (Dimocarpus longan Lour.) Cultivars (Yiduo × Shixia)

Genome-wide identification of Cellulose-like synthase D gene family in Dendrobium catenatum

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