Sensitive detection of MCF-7 human breast cancer cells by using a novel DNA-labeled sandwich electrochemical biosensor

Nucleic Acids for Electrochemical Biosensor Technology

“…In biosensors developed with DNA, the properties of DNA hybrids can be used. Yang et al [19] have developed an electrochemical biosensor for the determination of MCF-7 cells, which are breast cancer cells. In this biosensor, DNA molecules were used for modification.…”

Application of electrochemical biosensors in tumor cell detection

“…The basic principle is that the antigen‐antibody, as a molecular recognition element, is in direct contact with the electrochemical sensing element and converts the signal of a certain or a certain kind of chemical concentration into the corresponding electrical signal through the sensing element (Fig a). Therefore, biosensors constructed on the basis of specific reactions between antigens and antibodies, specific recognition function of adapters and the cell impedance principle have successfully achieved high‐sensitivity linear detection of various cancer cells, including human liver cancer cell line HepG2, human breast cancer cell line MCF‐7, small cell lung cancer, lung adenocarcinoma, squamous cell cancer, skin cancer, prostate cancer and breast cancer . In addition, glycosyls on the cell surface play an important role in cell recognition, adhesion, immune response, and the occurrence and migration of cancer cells .…”

“…Therefore, biosensors constructed on the basis of specific reactions between antigens and antibodies, specific recognition function of adapters and the cell impedance principle have successfully achieved high-sensitivity linear detection of various cancer cells, including human liver cancer cell line HepG2, human breast cancer cell line MCF-7, small cell lung cancer, lung adenocarcinoma, squamous cell cancer, skin cancer, prostate cancer and breast cancer. [39][40][41] In addition, glycosyls on the cell surface play an important role in cell recognition, adhesion, immune response, and the occurrence and migration of cancer cells. [42][43][44] Therefore, the cell electrochemical sensor based on surface-specific glycosyls of cancer cells has also successfully achieved the specific recognition and linear detection of HeLa cells, polysaccharide-rich K562 leukemia cells, and MDA-MB-231 cells.…”

Electrochemical Nanobiosensors for Detection of Breast Cancer Biomarkers

“…The aptasensor determined MCF-7 cells in the range of 328–593 cells/mL with LOD of 328 cells/mL, when cells were lysed and an intracellular level of platelet-derived growth factor was electrochemically determined [ 132 ]. Yang with colleagues [ 133 ] utilized GCE modified by several nanomaterials using a layer-by-layer deposition process incorporating 3D graphene, Au nanocages, and MWCNTs to which primary antibodies were immobilized (Approach 1). Once the biosensor was incubated with MCF-7 cells, a sandwich configuration was established by incubation with secondary antibodies linked to DNA.…”

“…In the next step, complementary DNA was applied, and to double-stranded DNA, methylene blue as a redox mediator was intercalated and detected using SWV. The biosensor detected BC cells with LOD of 80 cells/mL (a linear range of 1.0 × 10 2 –1.0 × 10 6 cells/mL) and exhibited satisfactory stability [ 133 ]. Wang et al [ 134 ] fabricated a sensitive sandwich-based aptamer biosensor for the label-free electrochemical detection of cells (Approach 2).…”