Graphene oxide nano-bio interaction induces inhibition of spermatogenesis and disturbance of fatty acid metabolism in the nematode Caenorhabditis elegans

Kim, Yong-Soon; Jeong, Jaeseong; Yang, Jaesung; Joo, Sang‐Woo; Ji, Hong; Choi, Jinhee

doi:10.1016/j.tox.2018.09.006

Cited by 34 publications

(28 citation statements)

References 59 publications

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“…Signals from reproductive tissues regulate the lifespan of many organisms [ 32 ]. Germline signals regulate stress resistance, lipid metabolism, and lifespan through nuclear transcription factors DAF-16, DAF-12, and NHR-80 [ 33 – 35 ]. Here, to test if SDG could affect the transcriptional activity of these transcription factors, we analyzed the mRNA level of genes downstream of DAF-16, DAF-12, and NHR-80 in C. elegans , including sod-3 , fard-1 , and fat-6 [ 36 ], respectively.…”

Section: Resultsmentioning

confidence: 99%

Secoisolariciresinol Diglucoside Delays the Progression of Aging-Related Diseases and Extends the Lifespan ofCaenorhabditis elegansvia DAF-16 and HSF-1

Tan

Zhou

et al. 2020

Oxidative Medicine and Cellular Longevity

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Secoisolariciresinol diglucoside (SDG) is a phytoestrogen and rich in food flaxseed, sunflower seeds, and sesame seeds. Among the beneficial pharmacological activities of SDG on health, many are age related, such as anticancer, antidiabetes, antioxidant, and neuroprotective effects. Thus, we investigated if SDG had an effect on antiaging in Caenorhabditis elegans (C. elegans). Our results showed that SDG could extend the lifespan of C. elegans by up to 22.0%, delay age-related decline of body movement, reduce the lethality of heat and oxidative stress, alleviate dopamine neurodegeneration induced by 6-hydroxydopamine (6-OHDA), and decrease the toxicity of Aβ protein in C. elegans. SDG could increase the expression of the downstream genes of DAF-16, DAF-12, NHR-80, and HSF-1 at mRNA level. SDG could not extend the lifespan of mutants from genes daf-16, hsf-1, nhr-80, daf-12, glp-1, eat-2, and aak-2. The above results suggested that SDG might enhance the stress resistance, delay the progression of aging-related diseases, and extend the lifespan of C. elegans via DAF-16 and HSF-1.

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Section: Resultsmentioning

confidence: 99%

Secoisolariciresinol Diglucoside Delays the Progression of Aging-Related Diseases and Extends the Lifespan ofCaenorhabditis elegansvia DAF-16 and HSF-1

Tan

Zhou

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…Therefore, ROS production is studied, as it can be a highly sensitive endpoint used for bio-safety assessment of specific NPs in C. elegans [31]. In the present study, any of the tested NPs produced ROS accumulation, even when oxidative stress damage presents the prime mechanism causing toxic effects after testing other different nanoparticles [31,52]. This clearly indicates that ROS production may be specific and will not only depend on NP size, but also on NP chemical composition.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, none of the NPs suppressed the reproductive process in C. elegans , implying no toxicity or different effects on worms’ reproduction. Nevertheless, the worms may have been affected by the undoped NPs through the sequential development period, including spermatogenesis, gonadogenesis and oogenesis by inducing aging of the reproductive system [52]. It is also important to consider that C. elegans has the capacity to adapt or acclimate to the presence of NPs across multiple generations [31].…”

Section: Discussionmentioning

confidence: 99%

“…Oxidative stress is considered to be the main mechanism by which NPs cause toxicity. In other studies, accumulation of reactive oxygen species (ROS) is usually observed in C. elegans treated with NPs [31,52]. This may result in increased lethality, decreased body growth, altered development, intestinal impairment, and reproductive affectation in a concentrated manner.…”

Section: Discussionmentioning

confidence: 99%

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Novel Polymeric Nanocarriers Reduced Zinc and Doxycycline Toxicity in the Nematode Caenorhabditis elegans

et al. 2019

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The objective was to evaluate the toxicity of zinc- and doxycycline-loaded polymeric nanoparticles (NPs) using Caenorhabditis elegans as a model organism. These NPs are composed of ethylene glycol dimethacrylate, 2-hydroxyethyl methacrylate and methacrylic acid. NPs were loaded with doxycycline (D-NPs) and zinc (Zn-NPs) by chemical adsorption, and loading efficacy was demonstrated. Worm death rate in a concentration-response curve basis was calculated for lethality. Metabolism was evaluated through pharyngeal pumping assay. Body length measurements, brood size and egg lays were used to gauge growth, reproduction and fertility respectively. Intracellular hydrogen peroxide levels were determined to assess the reactive oxygen species production. One-way ANOVA and Bonferroni were used for comparisons (p < 0.05). Tested NPs at the highest dosage did not affect lethality or worm metabolism, expressed in terms of death rate and pharyngeal pumping per minute, respectively. Zn-NPs slightly increased worm growth. The concentration of the intracellular hydrogen peroxide levels was the lowest in the D-NPs group. The distinct NPs and concentrations employed were shown to be non-toxic for in situ administration of zinc and doxycycline, reducing the harmful effects of these compounds.

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“…The survival and reproduction followed a similar response pattern and, hence, effects on reproduction appear to be a direct consequence of decreased survival rather than a specific reproductive impairment. In C. elegans , and as opposed to this, Kim et al (2018) suggested that GO exposure caused reproductive toxicity by suppressing spermatogenesis of C. elegans hermaphrodites during development, resulting in decreased sperm numbers and progeny numbers [28].…”

Section: Discussionmentioning

confidence: 99%

Graphene-Based Nanomaterials in Soil: Ecotoxicity Assessment Using Enchytraeus crypticus Reduced Full Life Cycle

et al. 2019

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Graphene-based nanomaterials (GBNs) possess unique physicochemical properties, allowing a wide range of applications in physical, chemical, and biomedical fields. Although GBNs are broadly used, information about their adverse effects on ecosystem health, especially in the terrestrial environment, is limited. Therefore, this study aims to assess the toxicity of two commonly used derivatives of GBNs, graphene oxide (GO) and reduced graphene oxide (rGO), in the soil invertebrate Enchytraeus crypticus using a reduced full life cycle test. At higher exposure concentrations, GO induced high mortality and severe impairment in the reproduction rate, while rGO showed little adverse effect up to 1000 mg/kg. Collectively, our body of results suggests that the degree of oxidation of GO correlates with their toxic effects on E. crypticus, which argues against generalization on GBNs ecotoxicity. Identifying the key factors affecting the toxicity of GBNs, including ecotoxicity, is urgent for the design of safe GBNs for commercial purposes.

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Graphene oxide nano-bio interaction induces inhibition of spermatogenesis and disturbance of fatty acid metabolism in the nematode Caenorhabditis elegans

Cited by 34 publications

References 59 publications

Secoisolariciresinol Diglucoside Delays the Progression of Aging-Related Diseases and Extends the Lifespan ofCaenorhabditis elegansvia DAF-16 and HSF-1

Secoisolariciresinol Diglucoside Delays the Progression of Aging-Related Diseases and Extends the Lifespan ofCaenorhabditis elegansvia DAF-16 and HSF-1

Novel Polymeric Nanocarriers Reduced Zinc and Doxycycline Toxicity in the Nematode Caenorhabditis elegans

Graphene-Based Nanomaterials in Soil: Ecotoxicity Assessment Using Enchytraeus crypticus Reduced Full Life Cycle

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