Assessment of acute, 14-day, and 13-week repeated oral dose toxicity of Tiglium seed extract in rats

Yun, Jun Won; Kwon, Euna; Kim, Yun Soon; Kim, Seung Hyun; You, Ji Ran; Kim, Hyoung Chin; Park, Jin-Sung; Hwan, Jeong; Lee, Sang Koo; Jang, Ja June; Kim, Hyeon Hoe; Kang, Byeong Cheol

doi:10.1186/s12906-018-2315-5

Cited by 7 publications

(6 citation statements)

References 52 publications

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“…Protein profile and metabolic biomarkers were also measured. Serum levels of three enzymes (ALT, AST, and ALP) are commonly used as clinical biochemistry markers associated with liver damage [ 33 – 35 ]. Among these enzymes, serum levels of ALT and AST of the groups 500, 1000, and 2000 mg/kg/bw were statistically lower when compared to the control.…”

Section: Discussionmentioning

confidence: 99%

Acute and Subacute Toxicity Studies of the Aqueous Extract from Haloxylon scoparium Pomel (Hammada scoparia (Pomel)) by Oral Administration in Rodents

Kharchoufa

Bencheikh

Assri

et al. 2020

BioMed Research International

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Ethnopharmacological Relevance. Haloxylon scoparium Pomel is a herbal medicine traditionally used for treating scorpions and snakebite, diabetes, and stomachache as well as several other diseases. No systematic study of the potential toxicity of the plant has been described. Aim of the Study. The current study is aimed at assessing the potential toxicity of Haloxylon scoparium Pomel through the acute and subacute toxicity tests. Materials and Methods. Acute toxicity test was performed on Swiss albino mice at a single oral dose of 1-10 g/kg for 14 consecutive days. General behavioral adverse effects, mortality, and latency of mortality were determined. In the subacute study, the Haloxylon scoparium Pomel extract was administered orally at doses of 500, 1000, and 2000 mg/kg daily for 30 days to Wistar rats. Body weight and selected biochemical and hematological parameters were determined at the end of the experiment. Sections of livers and kidneys were removed for histological studies. Results. Acute toxicity study showed that the oral LD50 value of Haloxylon scoparium Pomel extract was 5000 mg/kg. The subacute toxicity study of Haloxylon scoparium Pomel extract at doses 500, 1000, and 2000 mg/kg did not produce any observable symptoms of toxicity and no significant variation in body weight, organ weights, food, and water consumption or mortality in all treated rats. However, the administration of the Haloxylon scoparium Pomel extract to rats at 500 mg/kg and 1000 mg/kg showed a significant decrease in platelets. Moreover, only at the highest dose (2000 mg/kg), the extract caused a significant increase in red blood cells and hemoglobin. Our results showed that subacute treatments with Haloxylon scoparium Pomel extract at doses of 1000 mg/kg and 2000 mg/kg significantly elevated alkaline phosphatase and triglycerides. Histological studies showed that the subacute treatments of rats with Haloxylon scoparium Pomel extracts, at the doses 1000 and 2000 mg/kg, induced some histopathological changes in the livers but a slight changing in kidneys. Conclusion. Our results indicated low acute toxicity of the aqueous extract of Haloxylon scoparium Pomel. Furthermore, daily oral administration of Haloxylon scoparium Pomel extract caused some damages to the livers of rats treated with high doses, expressed by an increase in some enzyme activities such as ALP. Regarding the renal function, we did not find remarkable toxicity in the subacute treatment with Haloxylon scoparium Pomel extracts at doses 1000 and 2000 mg/kg. However, further toxicity assessments should be done to ascertain the safety or the toxicity of this valuable plant species “Haloxylon scoparium pomel” in subchronic treatments.

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Section: Discussionmentioning

confidence: 99%

Acute and Subacute Toxicity Studies of the Aqueous Extract from Haloxylon scoparium Pomel (Hammada scoparia (Pomel)) by Oral Administration in Rodents

Kharchoufa

Bencheikh

Assri

et al. 2020

BioMed Research International

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“…In order to evaluate the toxicity of any new compound, it is necessary to understand the status of these two important organs, which can be validated through biochemical evaluation ( Sureshkumar et al, 2018 ). Serum levels of three enzymes (ALT, AST, and ALP) are commonly used in studies as clinical biochemical markers related to liver injury ( Yun et al, 2018 ). Our results showed no significant differences in their serum levels between all groups.…”

Section: Discussionmentioning

confidence: 99%

Safety assessment of Acori Tatarinowii Rhizoma: acute and subacute oral toxicity

Liu,

Ping,

Sun

et al. 2024

Front. Pharmacol.

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Introduction: Acori Tatarinowii Rhizoma (ATR) is a well-known traditional Chinese medicine that is used for treating neuropathic diseases. However, there is little information about the safety of ATR.Methods: The present study evaluated the acute and subacute oral toxicity of a water extract of ATR in Institute of Cancer Research (ICR) mice. In acute trials, a single administration of extract at a dose 5,000 mg/kg body weight led to no clinical signs of toxicity or mortality, indicating that the lethal dose (LD50) exceeded 5,000 mg/kg. A subacute toxicity test was done using daily doses of 1,250, 2,500, and 5,000 mg/kg of the ATR extract for 28 days, which did not show any adverse clinical symptoms or mortality. However, the male renal organ index and urea level in mice given 5,000 mg/kg was obviously abnormal, which was consistent with pathological results and suggested that this dose might cause kidney injury.Results: Doses of ATR lower than 2,500 mg/kg could be regarded as safe, although the potential cumulative effects of long-term use of high doses of ATR need to be considered.Discussion: The study highlights the function of ATR in reducing blood lipids and provides a new idea for its widespread clinical use in the future.

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“…Phytochemical analysis by LC-MS/MS revealed the presence of nine alkaloids in the hydroethanolic extracts: trisphaeridine, galanthamine, crinine, demethylmaritidine, anhydrolycorine, nor-galanthamine, N-formylnorgalanthamine, peramine, and ergovaline. Several studies have identified the presence of alkaloids, but no study has identified these alkaloid types [ 45 ]. Sterols, saponins, and phenolic compounds have all been reported as secondary metabolites of this plant.…”

Section: Discussionmentioning

confidence: 99%

“…Sterols, saponins, and phenolic compounds have all been reported as secondary metabolites of this plant. A recent study demonstrated that D. ambrosioides contains alkaloids [ 45 ]. This finding correlates with our results.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Acute and Subacute Toxicity and LC-MS/MS Compositional Alkaloid Determination of the Hydroethanolic Extract of Dysphania ambrosioides (L.) Mosyakin and Clemants Flowers

Kandsi

Lafdil

Elbouzidi

et al. 2022

Toxins

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Dysphania ambrosioides (L.) Mosyakin and Clemants is a medicinal plant that has traditionally been used to cure a range of diseases. There has been no thorough investigation of the potential toxicity of this plant. The objective of this study is to assess the acute and subacute toxicity of D. ambrosioides hydroethanolic extract (DAHE), as well as it alkaloids composition, utilizing LC-MS/MS analysis. An in silico approach was applied to determine pharmacokinetic parameters and to predict the toxicity of D. ambrosioides identified alkaloids. A 14-day treatment with a single oral dose of 1–7 g/kg was carried out to investigate acute toxicity. DAHE was given orally at dosages of 5, 50, and 500 mg/kg for 15 days in the subacute toxicity investigation, and body weight and biochemical parameters were evaluated. Livers, kidneys, lungs, and heart were examined histologically. Chromatographic investigation revealed the existence of nine alkaloids, with N-formylnorgalanthamine being the most prevalent. The oral LD50 value of DAHE was found to be 5000 mg/kg in an acute toxicity study. No variations were observed with respect to food intake, water consumption, mortality, or body and organ weight in the subacute toxicity study. On the other hand, DAHE (500 mg/kg) significantly enhanced alanineaminotransferase, aspartate aminotransferase, and urea. Liver and kidney histological examinations revealed modest infiltration of hepatocyte trabeculae by inflammatory cells in the liver and slight alteration in the kidney histoarchitecture. According to our findings, DAHE exhibits low to moderate toxicity.

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Assessment of acute, 14-day, and 13-week repeated oral dose toxicity of Tiglium seed extract in rats

Cited by 7 publications

References 52 publications

Acute and Subacute Toxicity Studies of the Aqueous Extract from Haloxylon scoparium Pomel (Hammada scoparia (Pomel)) by Oral Administration in Rodents

Acute and Subacute Toxicity Studies of the Aqueous Extract from Haloxylon scoparium Pomel (Hammada scoparia (Pomel)) by Oral Administration in Rodents

Safety assessment of Acori Tatarinowii Rhizoma: acute and subacute oral toxicity

Evaluation of Acute and Subacute Toxicity and LC-MS/MS Compositional Alkaloid Determination of the Hydroethanolic Extract of Dysphania ambrosioides (L.) Mosyakin and Clemants Flowers

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