Genetic characterisation of methicillin-resistant Staphylococcus aureus and Staphylococcus pseudintermedius in pets and veterinary personnel in Iran: new insights into emerging methicillin-resistant S. pseudintermedius (MRSP)

Tabatabaei, Saeid; Najafifar, Arash; Badouei, Mahdi Askari; Salehi, Taghi Zahraei; Tamai, Iradj Ashrafi; Khaksar, Ehsan; Abbassi, Mohamed Salah; Ghazisaeedi, Fereshteh

doi:10.1016/j.jgar.2018.08.022

Cited by 22 publications

(16 citation statements)

References 37 publications

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“…Our results also agree with existing literature from others countries where carriage rates of MRSP in dogs ranged between 3-34% for studies reported from Spain, Finland, Iran and Canada (15,(36)(37)(38). MRSP is isolated less often in cats with carriage prevalence between 4-19% as reported in the United States,…”

Section: Discussionsupporting

confidence: 92%

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Epidemiology of methicillin resistant Staphylococcus species carriage in companion animals in the Greater Brisbane Area, Australia

Rynhoud

Meler

Gibson

et al. 2020

Preprint

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Background: Methicillin resistant Staphylococcus species such as S. aureus (MRSA) and S. pseudintermedius (MRSP) can be involved in life-threatening multidrug resistant infections in companion animals. Knowledge of methicillin resistant Staphylococcus (MRS) carriage and factors influencing carriage in companion animals from South East Queensland is limited. Nasal and rectal swab samples were collected from dogs, cats and horses upon admission or within 24 hours of hospitalisation to several primary accession and referral veterinary practices between November 2015 and December 2017. MRSA and MRSP were identified using standard microbiological (Brilliance selective medium) and molecular (mecA gene PCR) methods. Risk factors associated with methicillin resistant Staphylococcus (MRS) carriage were quantified using Bernoulli logistic regression models. A Bayesian geostatistical model was developed to predict the probability of MRS carriage in Brisbane and surrounding areas. Results: Our results indicated that while the prevalence of MRSP carriage in dogs was 8.7% (35/402) no MRSP was isolated from cats (0/69) and horses (0/60); no MRSA was isolated in any species. MRSP carriage in dogs was significantly associated with previous hospitalisation, previous bacterial infection, consultation type, average precipitation, and human population density. Our predictive map of MRSP carriage indicated that the probability of carriage was highest along the coastal areas of Greater Brisbane, particularly Brisbane city, Sunshine Coast and Gympie areas. Conclusions: This study determined that MRSP carriage in dog populations from South East Queensland is geographically clustered and associated with both clinical and environmental factors.

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Section: Discussionsupporting

confidence: 92%

“…Brazil and Iran (38,39,40). There is currently no MRSP carriage being reported in horses; however, it has been identi ed from clinical infections (41)(42)(43).…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of methicillin resistant Staphylococcus species carriage in companion animals in the Greater Brisbane Area, Australia

Rynhoud

Meler

Gibson

et al. 2020

Preprint

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“…Staphylococcus pseudintermedius (SP) is a Gram-positive bacterium that can act both as a skin commensal or a pathogen and is nowadays considered one of the main causative agents of canine pyoderma [1,2]. It is also able to cause ear, wound, and post-surgical infections in domestic animals, particularly in dogs [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Efficacy of Essential Oils from Melaleuca Alternifolia and Rosmarinus Officinalis, Manuka Honey-based Gel, and Propolis as Antibacterial Agents Against Canine Staphylococcus Pseudintermedius Strains

et al. 2020

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Essential oils (EOs) and honeybee products (e.g., honey and propolis) are natural mixtures of different volatile compounds that are frequently used in traditional medicine and for pathogen eradication. The aim of this study was to evaluate the antibacterial properties of tea tree (Melaleuca alternifolia) EO (TTEO), Rosmarinus officinalis EO (ROEO), manuka-based gel, and propolis against 23 strains of Staphylococcus pseudintermedius (SP) isolated from canine pyoderma. Antimicrobial resistance screening was assessed using a panel of nine antimicrobial agents coupled with a PCR approach. An aromatogram was done for both EOs, using the disk diffusion method. The minimum inhibitory concentration (MIC) was determined for all the compounds. Among the 23 SP strains, 14 (60.9%) were multidrug-resistant (MDR), 11 strains (47.8%) were methicillin-resistant (MRSP), and 9 (39.1%) were non-MDR. The mean diameter of the inhibition zone for Melaleuca and Rosmarinus were 24.5 ± 8.8 mm and 15.2 ± 8.9 mm, respectively, resulting as statistically different (p = 0.0006). MIC values of TTEO and ROEO were similar (7.6 ± 3.2% and 8.9 ± 2.1%, respectively) and no statistical significances were found. Honeybee products showed lower MIC compared to those of EOs, 0.22 ± 0.1% for Manuka and 0.8 ± 0.5% for propolis. These findings reveal a significant antibacterial effect for all the tested products.

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“…Os resultados obtidos nas análises microbiológicas são preocupantes para a saúde dos consumidores desse tipo de queijo quando se associa o número de UFC/g e a possibilidade de contaminação por S. aureus, este sendo a espécie de SCP de maior risco para saúde humana e animal (Tabatabaei et al, 2019). Quanto maior a contaminação por Staphylococcus spp., maior a possibilidade da presença de S. aureus.…”

Section: Discussionunclassified

Avaliação da contaminação por Staphylococcus aureus em queijo coalho artesanal elaborado com leite de cabra produzido no estado de Pernambuco

Aragão

Trajano

Silva

et al. 2020

Arq. Bras. Med. Vet. Zootec.

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RESUMO A fabricação de queijo coalho artesanal elaborado com leite de cabra é composta pelas etapas de obtenção do leite, refrigeração, manipulação e armazenamento, que aumentam o risco de contaminação do produto. Objetivou-se neste estudo avaliar o nível de contaminação por Staphylococcus aureus em amostras de queijo coalho artesanal produzido com leite de cabra cru no estado de Pernambuco, Brasil, bem como avaliar a concordância entre a técnica oficial da Instrução Normativa nº62/2003 (Mapa) e a técnica molecular (gene nuc) para identificar S. aureus no queijo. Houve crescimento de colônias típicas de Staphylococcus aureus em 100% das amostras, e a contagem variou de 7,0×103 a 8,6×106 UFC/g. Das 30 amostras analisadas, 18 (60,0%) apresentaram valores superiores ou iguais a 105UFC/g, e 21 (70,0%) estavam contaminadas por S. aureus. A concordância entre os métodos de diagnóstico de S. aureus em queijo coalho caprino foi moderada. O nível de contaminação dos queijos revela a necessidade de ações de melhoria das condições de elaboração do produto, a fim de garantir um produto seguro aos consumidores.

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Genetic characterisation of methicillin-resistant Staphylococcus aureus and Staphylococcus pseudintermedius in pets and veterinary personnel in Iran: new insights into emerging methicillin-resistant S. pseudintermedius (MRSP)

Cited by 22 publications

References 37 publications

Epidemiology of methicillin resistant Staphylococcus species carriage in companion animals in the Greater Brisbane Area, Australia

Epidemiology of methicillin resistant Staphylococcus species carriage in companion animals in the Greater Brisbane Area, Australia

In Vitro Efficacy of Essential Oils from Melaleuca Alternifolia and Rosmarinus Officinalis, Manuka Honey-based Gel, and Propolis as Antibacterial Agents Against Canine Staphylococcus Pseudintermedius Strains

Avaliação da contaminação por Staphylococcus aureus em queijo coalho artesanal elaborado com leite de cabra produzido no estado de Pernambuco

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