Novel amphiphilic polyvinylpyrrolidone functionalized silicone particles as carrier for low-cost lipase immobilization

Abstract

The high catalytic activity, specificity and stability of immobilized lipase have been attracting great interest. How to reduce the cost of support materials has always been a hot topic in this field. Herein, for the development of low-cost immobilized lipase, we demonstrate an amphiphilic polyvinylpyrrolidone (PVP) grafted on silicone particle (SP) surface materials (SP-PVP) with a rational design based on interfacial activation and solution polymerization. Meanwhile, hydrophilic pristine SP and hydrophobic polystyrene-corded silicone particles (SP-Pst) were also prepared for lipase immobilization. SP-PVP was characterized by X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and thermogravimetry. Our results indicated that the lipase loading amount on the SP-PVP composites was about 215 mg of protein per gram. In the activity assay, the immobilized lipase SP-PVP@CRL exhibited higher catalysis activity and better thermostability and reusability than SP@CRL and SP-Pst@CRL. The immobilized lipase retained more than 54% of its initial activity after 10 times of re-use and approximately trended to a steady rate in the following cycles. By introducing the interesting amphiphilic polymer to this cheap and easily obtained SP surface, the relative performance of the immobilized lipase can be significantly improved, facilitating interactions between the low-cost support materials and lipase.

Comparative Study on Lipase Immobilized onto Organo-Cation Exchanged Kaolin and Metakaolin: Surface Properties and Catalytic Activity

“…Immobilization of CRL had caused decrease in the intensities of the XRD patterns as a result of CRL loading (Figure 3(c) and Figure 3(d)), which had led to decrease in the surface crystallinity. However, XRD patterns were found to remain as it was before the immobilization process, and this is in agreement with the finding by Zhang et al [44].…”

Pharmaceutical assessment of polyvinylpyrrolidone (PVP): As excipient from conventional to controlled delivery systems with a spotlight on COVID-19 inhibition

“…The rational design is based on the interfacial activation and solution polymerization. The activity assay revealed that lipase immobilized with PVP functionalized silicone particles has exhibited higher catalysis activity and also better thermostability and reusability compared with hydrophilic pristine or hydrophobic polystyrene corded silicone particles [ 158 ]. Other examples include asparaginase, catalase, galactosidase, etc.…”

Synthesis of lutein esters using a novel biocatalyst of Candida antarctica lipase B covalently immobilized on functionalized graphitic carbon nitride nanosheets

“…13 Immobilizing lipase on various carriers in different ways, can promise the function of improving the thermal stability and reusability, leading to lower cost of lipase application. 14,15 However, very few papers have described the enzymatic synthesis of lutein esters. Wang 16 and Hou 17 tried to catalyze the synthesis of lutein palmitate by Novozyme 435 and lipase-Pluronic F-127.…”