A microchip electrophoresis-based assay for ratiometric detection of kanamycin by R-shape probe and exonuclease-assisted signal amplification

Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin

“…S4, lanes 1, 2 and 4 presented a bright band, whereas the lane 3, owing to the hydrolysis of the anti-kanamycin aptamer and/or cDNA by Exo I and then migrated out of the gel. This result confirms that the kanamycin aptamer can be cleaved upon binding kanamycin, which is consistent with the previous studies (Chen et al, 2018;Zeng, Tang, Zhang, Luo, & Tang, 2018).…”

“…3B showed that the absorption ratio is linear in the range of 10 − 13 to 10 − 8 M in the concentration range of kanamycin. The limit of detection (LOD) value was calculated to be 2.8 × 10 − 14 M according to the 3σ/s method (LOD = 3σ/s, σ is the standard deviation of the blank sample, and s is the slope of the standard curve), which is at least 1 orders of magnitude lower than that of reported colorimetry methods as well as other detection methods (Table S1) (Blanchaert et al, 2013;Chen et al, 2018;Han et al, 2019;Hao et al, 2016;Ramezani et al, 2016;Saratale et al, 2020;Xu et al, 2015;Yu et al, 2013).…”

“…Among these signal amplification strategies, nuclease-assisted signal amplification technology designed by combining the cycle effect of nuclease digestion, aptamer and nanotechnology has been applicated frequently in biochemical analysis due to its merits of simple operation, high sensitivity and specificity, as well as relatively short reaction time (Liu et al, 2013). There are two main types of signal amplification strategies based on nuclease assisted signal amplification (Chen et al, 2018;Zhang et al, 2019): one is the sequence dependent signal amplification strategy with restriction enzyme or restriction enzyme assisted signal amplification; the other is the signal amplification strategy without sequence dependent nuclease assisted signal amplification. In terms of the universality of nuclease-assisted signal amplification strategy, the exonuclease without sequence dependence is simpler and more feasible in the aspect of probe design and target analysis, thus which shows a broader application prospect.…”

Photoelectrochemical Aptasensors Constructed with Photosensitive PbS Quantum Dots/TiO2 Nanoparticles for Detection of Kanamycin

“…Up to the present, a series of analytical techniques have been proposed to detect Kana, for example, high-performance liquid chromatography, capillary electrophoresis, spectrophotometry, , enzyme-linked immunosorbent assay . Although these methods can effectively detect residual Kana, they are limited by cumbersome operations, time-consuming, expensive equipment, low sensitivity or specificity.…”

Current advances in immunoassays for the detection of antibiotics residues: a review

“…The amount of target can be converted quantitatively to the intensity of DNA deprived of labelling with MCE and attained exonuclease-assisted signal in the solution by the R-DNA probe. This assay offers an important platform for the rapid detection of antibiotic residues in food matrices (Chen et al, 2018). Another flow-through chemiluminescence microarray was conducted which can simultaneously detect 13 antibiotics in milk (Meyer et al, 2017).…”