2D vs. 3D Cell Culture Models for In Vitro Topical (Dermatological) Medication Testing

Teimouri, Arezou; Yeung, P. K. F.; Agu, Remigius U.

doi:10.5772/intechopen.79868

Cited by 17 publications

(23 citation statements)

References 68 publications

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“…In this way, we have selected the direct contact evaluation with the traditionally cell cultures of planar, static 2D models and the new 3D model on trans-well culture system in order to bring new evidences for helping this area of research. Most probably, the next step in improving the methodology of textiles’ cytotoxicity assessment will be the use of 3D bioprinted skin models, providing a more uniform model which better mimic the in vivo skin and with lower costs of production compared to currently available models on the market, such as Epiderm™, SkinEthic RHE™, and EpiSkin™ [43].…”

Section: Resultsmentioning

confidence: 99%

Essential Oil Microcapsules Immobilized on Textiles and Certain Induced Effects

et al. 2019

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In order to obtain textile materials with potential utility in the development of cosmetic textiles, this study examined the deposition by padding of rose and sage microcapsules on woven textile structures, with different fiber compositions (100% cotton and 50% cotton/50% polyester). Cationization of the textile materials was performed to enhance the degree of uptake the pf the microcapsules on the fabrics’ surface. A commercially acrylate-based binder was used to fix the microcapsules to the textile substrate and to improve the durability against external factors. The finished textile materials were characterized in terms of their physical-mechanical characteristics. The distribution of microcapsules on the fabrics surface before and after five washing cycles and 1000 abrasion cycles was investigated by scanning electron microscopy. The biocompatibility in terms of cell viability, cell membrane integrity and inflammation status of the functionalized fabrics was evaluated on CCD-1070Sk normal human dermal fibroblasts. The cell morphology was evaluated by F-actin staining using fluorescence microscopy and no significant changes were noticed after the incubation in the presence of fabrics compared with control. The in vitro biocompatibility evaluation on human skin cells confirmed the absence of cytotoxicity after the short-term exposure, supporting further in vivo use of these innovative textiles with improved properties.

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Section: Resultsmentioning

confidence: 99%

Essential Oil Microcapsules Immobilized on Textiles and Certain Induced Effects

et al. 2019

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“…The literature evidence clearly establishes the popularity and potential of in vitro 3D models containing both normal human keratinocytes and fibroblasts to closely mimic the physiological functions of the human skin. However, the main application of these models, so far, are limited to the assessment of skin irritation due to chemicals/drugs, creams and cosmetics, wound healing, skin ageing, and disease pathology [85,86]. The use of in vitro 3D models in permeation studies is limited due to the differences in lipid composition and organization in comparison to native skin.…”

Section: Artificial Cultured Human Skin Modelmentioning

confidence: 99%

Alternatives to Biological Skin in Permeation Studies: Current Trends and Possibilities

et al. 2020

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The transdermal route of drugs has received increased attention in recent years due to numerous advantages over the oral and injectable routes, such as avoidance of the hepatic metabolism, protection of drugs from the gastrointestinal tract, sustained drug delivery, and good patient compliance. The assessment of ex vivo permeation during the pharmaceutical development process helps in understanding the product quality and performance of a transdermal delivery system. Generally, excised human skin relevant to the application site or animal skin is recommended for ex vivo permeation studies. However, the limited availability of the human skin and ethical issues surrounding the use of animal skin rendered these models less attractive in the permeation study. In the last three decades, enormous efforts have been put into developing artificial membranes and 3D cultured human skin models as surrogates to the human skin. This manuscript provides an insight on the European Medicines Agency (EMA) guidelines for permeation studies and the parameters affected when using Franz diffusion cells in the permeation study. The need and possibilities for skin alternatives, such as artificially cultured human skin models, parallel artificial membrane permeability assays (PAMPA), and artificial membranes for penetration and permeation studies, are comprehensively discussed.

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“…The first implemented skin model was a 2D keratinocyte monolayer culture derived from human skin. 6,7 Although 2D model is advantageous for its simplicity and batch-to-batch reproducibility, 8 it is not adapted to assess topical skin permeation. 9 Closer to the skin histological organization, 3D reconstructed skin models and blocks of excised human skin were used to assess the permeation of topically applied substances such as caffeine, salicylic acid or benzoic acid.…”

Section: Introductionmentioning

confidence: 99%

Combining Raman imaging and MCR‐ALS analysis for monitoring retinol permeation in human skin

Essendoubi

Alsamad

Noël

et al. 2021

Skin Research and Technology

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Background: Monitoring the transcutaneous permeation of exogenous molecules using conventional techniques generally requires long pre-analytical preparation or labelling of samples. However, Raman spectroscopy is a label-free and nondestructive method which provides spatial distribution of tracked actives in skin. The aim of our study was to prove the interest of Raman imaging coupled with multivariate curve resolution alternating least square (MCR-ALS) analysis in monitoring retinol penetration into frozen and living human skin. Materials and Methods:After topical treatment of skin samples by free or encapsulated retinol, thin cross sections were analysed by Raman imaging (up to 100 µm depth). Mann-Whitney test was used to identify retinol spectroscopic markers in skin. MCR-ALS was used to estimate retinol contribution in Raman spectral images.Heat maps were constructed to compare the distribution of free and encapsulated retinol in skin models. Results:We identified the bands at 1158, 1196 and 1591 cm -1 as specific features for monitoring retinol in skin. Moreover, our MCR-ALS results showed an improvement of retinol penetration (up to 30 µm depth) with the encapsulated form as well as storage reservoir formation in stratum corneum, for each skin model. Finally, greater retinol penetration into living skin was observed. Conclusion:This study shows a proof of concept for the evaluation of retinol penetration in skin using Raman imaging coupled with MCR-ALS. This concept needs to be validated on more subjects to include inter-individual variability but also other factors affecting skin permeation (age, sex, pH, etc). Our study can be extended to other actives.

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2D vs. 3D Cell Culture Models for In Vitro Topical (Dermatological) Medication Testing

Cited by 17 publications

References 68 publications

Essential Oil Microcapsules Immobilized on Textiles and Certain Induced Effects

Essential Oil Microcapsules Immobilized on Textiles and Certain Induced Effects

Alternatives to Biological Skin in Permeation Studies: Current Trends and Possibilities

Combining Raman imaging and MCR‐ALS analysis for monitoring retinol permeation in human skin

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