Molecular characterization and antimicrobial susceptibility pattern of Streptococcus agalactiae isolated from clinical mastitis in dairy cattle

Tomazi, Tiago; Filho, Antônio Francisco de Souza; Heinemann, Marcos Bryan; Santos, Marcos Veiga dos

doi:10.1371/journal.pone.0199561

Cited by 37 publications

(47 citation statements)

References 33 publications

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“…Data presented here, coupled with the data reported in our earlier study (Hoque et al, 2019) provide important insights into the resistance potentials in CM microbiomes. Our results are concordant with MDR bacteria reported elsewhere from the milk of clinically infected cows (Curone et al, 2018;Tomazi et al, 2018;Cheng et al, 2019), buffalo cows (Preethirani et al, 2015), and humans (Penders et al, 2013;Patel et al, 2017;Baron et al, 2018). Our findings linked multidrug resistance to efflux pumps (MREP), CmeABC operon, mdtABCD cluster, BlaR1 family, methicillin resistance in Staphylococcus (MRS), resistance to fluoroquinolones (RFL), and multiple metals resistance to CZCR and AR as the predominantly abundant antibiotics and toxic compounds resistance (RATC) functional groups in CM microbiomes, suggesting that bovine CM milk microbiome constitutes a good reservoir for AMR (Kumar and Varela, 2012;Penders et al, 2013;Baron et al, 2018;Hoque et al, 2019).…”

Section: Discussionsupporting

confidence: 93%

“…Bacteria residing in the bovine gastrointestinal tract and udder may become resistant to these antibiotics and, once released into the milk, they may take part in horizontal transfer of antibiotic resistance genes (ARGs) to other CM bacteria of contagious and environmental origin (Cheng et al, 2019;Zaheer et al, 2019). Furthermore, AMR is a global health concern in both human and veterinary medicine (Van Boeckel et al, 2015), and thus, monitoring the emergence of AMR strains is an essential component of bovine CM prevention and control strategies (Tomazi et al, 2018;Cheng et al, 2019). Therefore, finding an effective alternative strategy for the control of bovine mastitis is a challenge for dairy producers.…”

Section: Introductionmentioning

confidence: 99%

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Insights Into the Resistome of Bovine Clinical Mastitis Microbiome, a Key Factor in Disease Complication

et al. 2020

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Bovine clinical mastitis (CM) is one of the most prevalent diseases caused by a wide range of resident microbes. The emergence of antimicrobial resistance in CM bacteria is well-known, however, the genomic resistance composition (the resistome) at the microbiome-level is not well characterized. In this study, we applied whole metagenome sequencing (WMS) to characterize the resistome of the CM microbiome, focusing on antibiotics and metals resistance, biofilm formation (BF), and quorum sensing (QS) along with in vitro resistance assays of six selected pathogens isolated from the same CM samples. The WMS generated an average of 21.13 million reads (post-processing) from 25 CM samples that mapped to 519 bacterial strains, of which 30.06% were previously unreported. We found a significant (P = 0.001) association between the resistomes and microbiome composition with no association with cattle breed, despite significant differences in microbiome diversity among breeds. The in vitro investigation determined that 76.2% of six selected pathogens considered "biofilm formers" actually formed biofilms and were also highly resistant to tetracycline, doxycycline, nalidixic acid, ampicillin, and chloramphenicol and remained sensitive to metals (Cr, Co, Ni, Cu, Zn) at varying concentrations. We also found bacterial flagellar movement and chemotaxis, regulation and cell signaling, and oxidative stress to be significantly associated with the pathophysiology of CM. Thus, identifying CM microbiomes, and analyzing their resistomes and genomic potentials will help improve the optimization of therapeutic schemes involving antibiotics and/or metals usage in the prevention and control of bovine CM.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Insights Into the Resistome of Bovine Clinical Mastitis Microbiome, a Key Factor in Disease Complication

et al. 2020

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“…agalactiae (45 RAPDtypes) and three clusters (Ia, Ib and II) resulted from genetic similarity among genotypes. After clustering, high genetic similarity was recognized within and between herds (Tomazi et al, 2018).…”

Section: Random Amplification Of Polymorphic Dna (Rapd)mentioning

confidence: 99%

Comparative Diagnosis of Infectious Bacteria in Bovine Milk

Sedky¹,

Ghazy²,

Soliman³

et al. 2020

JAHP

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B ovine milk is a source of nutrition for people and calf since it contains the supplements necessary for growth and progress of the calf. It could be a source of lipids, proteins, amino acids, minerals and vitamins. It contains immunoglobulins, hormones, growth factors, cytokines, nucleotides and peptides (Haug et al., 2007). Raw bovine milk is considered as a suitable growth medium for the multiplication of nearly all kinds of microorganisms (Kumar et al., 2010; Mahmoud et al., 2019). Bovine milk is considered as a vehicle for many infectious bacterial diseases. The most common infectious bacterial diseases that spread via bovine milk to young calves and human are bovine tuberculosis, brucellosis, mycoplasma infection, leptospirosis, and Q fever.

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“…Staphylococcus (CNS; 9%) and S. agalactiae (4%) (OLIVEIRA et al, 2015). Another Brazilian study analyzed a total of 4,212 cases of CM, of which 44% comprised negative culture samples, and among the positive samples, gram-positive pathogens were the most commonly found (66.3%) (TOMAZI et al, 2018). The same authors reported a high frequency of contagious pathogens and environmental streptococci, especially S. uberis and S. agalactiae, respectively, followed by S. aureus.…”

Section: Causing Agentsmentioning

confidence: 99%

“…The dilution ranges for each ATM and the interpretation criteria for determining the susceptible, intermediate and resistant are shown inTable 12. Isolates with intermediate susceptibility were considered resistant(TOMAZI et al, 2018). Strains of S. aureus resistant to cefoxitin were classified as MRSA.…”

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confidence: 99%

>b<Molecular characterization of >i<Staphylococcus aureus>/i< and >i<Streptococcus uberis>/i< isolates of intramammary infections of dairy cows>/b<

Alves

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Aos maiores incentivadores da minha vida, que mesmo de longe torcem pela minha felicidade e pelo meu sucesso. De onde eu busco forças para nunca desistir e para recomeçar sempre que necessário. Os meus amados pais, Marlene e Hélvio. ACKNOWLEDGEMENTS A Deus, por ser luz em todos os meus dias, por ser presente quando as coisas ficam mais difíceis, por me dar força e calma sempre. E a todos seus intercessores, sejam eles físicos ou espirituais, que também sempre estão comigo. Aos meus pais, Marlene e Hélvio, por entenderem que a distância faria parte da conclusão de um sonho, me formar e ao fim, me tornar doutora. Vocês vão ser sempre meu motivo de querer evoluir, ser melhor a cada dia, e viver intensamente. Meu amor por vocês é imensurável. A Universidade de São Paulo, Campus Pirassununga, por me proporcionar tantos lugares maravilhosos, onde pude ter horas de estudo, lazer e bons momentos. Ao meu orientador Prof. Dr. Marcos Veiga dos Santos, por confiar no meu trabalho, por acreditar no meu potencial e ser exemplo de profissionalismo e empenho no que faz. Muito obrigada por esses anos de convivência e evolução. O presente trabalho foi inicialmente realizado com apoio da Coordenação de

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Molecular characterization and antimicrobial susceptibility pattern of Streptococcus agalactiae isolated from clinical mastitis in dairy cattle

Cited by 37 publications

References 33 publications

Insights Into the Resistome of Bovine Clinical Mastitis Microbiome, a Key Factor in Disease Complication

Insights Into the Resistome of Bovine Clinical Mastitis Microbiome, a Key Factor in Disease Complication

Comparative Diagnosis of Infectious Bacteria in Bovine Milk

>b<Molecular characterization of >i<Staphylococcus aureus>/i< and >i<Streptococcus uberis>/i< isolates of intramammary infections of dairy cows>/b<

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