Bacterial Colonization and Tissue Compatibility of Denture Base Resins

Abstract

Currently, there is minimal clinical data regarding biofilm composition on the surface of denture bases and the clinical tissue compatibility. Therefore, the aim of this experimental study was to compare the bacterial colonization and the tissue compatibility of a hypoallergenic polyamide with a frequently used PMMA resin tested intraorally in a randomized split-mouth design. Test specimens made of polyamide (n = 10) and PMMA (n = 10) were attached over a molar band appliance in oral cavity of 10 subjects. A cytological smear test was done from palatal mucosa at baseline and after four weeks. The monolayers were inspected for micronuclei. After four weeks in situ, the appliance was removed. The test specimens were immediately cultivated on non-selective and selective nutrient media. All growing colonies were identified using VITEK-MS. The anonymized results were analyzed descriptively. A total of 110 different bacterial species could be isolated, including putative pathogens. An average of 17.8 different bacterial species grew on the PMMA specimens, and 17.3 on the polyamide specimens. The highest number of different bacterial species was n = 24, found on a PMMA specimen. On the two specimens, a similar bacterial distribution was observed. Micronuclei, as a marker for genotoxic potential of dental materials, were not detected. This study indicates that the composition of bacterial biofilm developed on these resins after four weeks is not influenced by the type of resin itself. The two materials showed no cytological differences. This investigation suggests that polyamide and PMMA are suitable for clinical use as denture base material.

Heat-cured poly(methyl methacrylate) resin incorporated with different food preservatives as an anti-microbial denture base material

“…As described previously, denture base materials prone to be reservoir for microbial colonization from several pathogens. 3 , 4 , 18 In this study, the incorporation of food preservatives into the acrylic denture base was introduced to promote anti-microbial effect. Various food preservatives including zinc oxide (ZnO), potassium sorbate (PS), and sodium metabisulfite (SM) were preliminarily studied their optimal compositions which were mentioned in the previous patent.…”

“…1 , 2 However, the denture base materials are prone to be colonized by microorganisms in the oral environment, including Staphylococcus aureus , Pseudomonas aeruginosa and Candida albicans. 3 , 4 , 5 These can contribute diseases in the oral cavity, especially denture stomatitis that is one major problem among denture wearers. 6 Moreover, microbial infections can also cause systemic effects, such as pneumonia and endocarditis.…”

Microbiological and Immunological Effects of Monomer Free Thermosens and Nano Zirconia Oxide Reinforced Denture Base Resins on Controlled Diabetic Denture Wearers

“…This result is in agreement with previous study that also proved reduced microbial growth (45) . However, Olms et al (46) proved no difference in microbial colonization on polyamides denture bases compared to heat cure denture bases.…”

Biocompatibility of conventional acrylic resin versus modified with titanium dioxide nanoparticles in maxillofacial prosthesis. A parallel randomized control trial

“…By the evolution of the nanotechnology in the dental material, the mechanical properties of acrylic resins have been improved by chemical modification of existing PMMA with various nano fillers such as metallic powders, fibers, nanoparticles and nanorods. (10)(11)(12) Several studies had investigated the effect of adding titanium dioxide nanoparticles on the properties of PMMA. It was concluded that it could improve the flexure strength, fracture toughness, hardness, thermal conductivity of PMMA and it reduces the water sorption.…”

“…Cell cultures for toxicity testing of dental materials were found to be a valuable tool for understanding their biological behavior while the change in the microorganisms count and level of immunoglobulin A in the secretion of subjects using the obturator were the indicator of the acquired immunity changes. (12)(13)(14) The question raised in this study does the addition of titanium dioxide nanoparticles to the maxillofacial prosthesis will affect its biocompatibility?…”