The Draft Genome Sequence of Clostridium sp. Strain NJ4, a Bacterium Capable of Producing Butanol from Inulin Through Consolidated Bioprocessing

Abstract

A novel butanogenic Clostridium sp. NJ4 was successfully isolated and characterized, which could directly produce relatively high titer of butanol from inulin through consolidated bioprocessing (CBP). The assembled draft genome of strain NJ4 is 4.09 Mp, containing 3891 encoded protein sequences with G+C content of 30.73%. Among these annotated genes, a levanase, a hypothetical inulinase, and two bifunctional alcohol/aldehyde dehydrogenases (AdhE) were found to play key roles in the achievement of ABE production from inulin through CBP.

Clostridium sp. Strain NJ4: A Promising Solventogenic Strain for Butanol Production from Jerusalem Artichoke through Consolidated Bioprocessing

“…Nowadays, levanases have been commonly found in yeasts, filamentous fungi, and some bacteria, such as Actinomyces viscosus, Bacillus subtilis, etc. , Through blasting the non-redundant protein sequence database in the NCBI website, the protein sequence of hypothetical levanase from strain NJ4 shows 62% similarity with that from B. subtilis (KFF55392.1), which has been described in our previous study . In addition to levan, levanase also shows hydrolytic activity on inulin and sucrose, indicating that this enzyme may be responsible for inulin degradation .…”

“…27,28 Through blasting the non-redundant protein sequence database in the NCBI website, the protein sequence of hypothetical levanase from strain NJ4 shows 62% similarity with that from B. subtilis (KFF55392.1), which has been described in our previous study. 29 In addition to levan, levanase also shows hydrolytic activity on inulin and sucrose, indicating that this enzyme may be responsible for inulin degradation. 30 In addition, another protein annotated as fructan β-fructosidase in the KEGG database may also possess the capability of inulin degradation.…”

Consolidated bioprocessing performance of a two‐species microbial consortium for butanol production from lignocellulosic biomass

“…As known, ctfA and ctfB are usually two key genes for volatile fatty acids reutilization, and adhE 1 and adhE 2 play essential roles in butanol production (Jiang, Lu et al, 2018). To better understand the metabolic regulation of these genes in strain NJ4, the transcriptional levels of these four genes were measured by the RT‐qPCR.…”

“…T. thermosaccharolyticum M5 and C. acetobutylicum NJ4 were isolated in our lab. These two microorganisms were preserved in China Center for Type Culture Collection (CCTCC) with the deposit number of CCTCC M2017072 and CCTCC M20191080, respectively (Jiang, Liu et al, 2018; Jiang, Lu et al, 2018). These two strains were anaerobically cultivated at 55°C and 37°C in mineral salts medium, respectively.…”