High butanol production from galactose riched red seaweed Gracilaria lemaneiformis by solventogenic Clostridium sp. strain NJ4

Jiang, Yujia; Li, Jiawen; Ni, Yufan; Tang, Yunhan; Wu, Mengdi; Zhang, Wenming; Xin, Fengxue; Jiang, Min

doi:10.1016/j.fuel.2021.121867

Cited by 7 publications

(3 citation statements)

References 27 publications

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“…Besides, different microorganisms participate in converting sugar-rich hydrolysate to biobutanol by acetone-butanol-ethanol (ABE) fermentation. A variety of Clostridia species were reported to have been used to produce solvent mixtures, usually in the ratio of 3:6:1 acetone-butanol-ethanol, respectively (Jiang et al, 2022). However, the capacity of the fermentation microbe to achieve a high yield of fermentation products despite the presence of phenolic inhibitors and the fermentation products as well as the ability to utilise both hexose and pentose is one of the significant factors leading to the production economy and sustainability.…”

Section: P R E S Smentioning

confidence: 99%

EVALUATION OF OIL PALM FROND FOR BIOBUTANOL PRODUCTION BY DIFFERENT Clostridia SPECIES

ABUBAKAR

2023

JOPR

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Ideal substrates and biobutanol producers are the key and most important ingredients for efficient biobutanol production. In this research, we evaluated the composition of oil palm fronds based on the different ages of the plant. Fresh oil palm frond (OPF) juice was found to be the most potent OPF component for biobutanol production and was pressed from 5, 10, 15 and 20 yr old trees using a sugarcane pressing machine. The total sugar concentrations were estimated by the use of a DNS and spectrophotometer and were found to be decreasing with the increasing age of the palm oil tree, with the highest sugar obtained at OPF ages 5 and 10. HPLC analysis revealed that glucose was the highest fraction in the juice, accounting for 57% to 66%, while fructose (12%-17%) and sucrose (19%-25%) made up the remaining percentage. Fermentation inhibitors like gallic and ferulic acids were also detected in the juice using the HPLC technique, and their concentrations also increased with age. Therefore, the research screened for a robust bacterial strain that can tolerate these phenolics in the hydrolysate for biobutanol production. Out of the 11 strains of Clostridium screened, strain A1 was found to successfully utilise the hydrolysate and yield the highest concentration of biobutanol at 2.32 g/L with productivity of 0.064 g/L/hr. The strain was identified as Clostridium strain A1 based on 16S rRNA gene sequencing techniques.

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Section: P R E S Smentioning

confidence: 99%

EVALUATION OF OIL PALM FROND FOR BIOBUTANOL PRODUCTION BY DIFFERENT Clostridia SPECIES

ABUBAKAR

2023

JOPR

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“…The red algae hydrolysate contains three major sugars, galactose, AHG, and glucose. Glucose is rapidly consumed and leads to catabolite repression of galactose consumption, , which is inherently a slow metabolic process. Due to the complicated enzymatic process, the rate of D-Gal metabolism in the Leloir pathway is quite slow .…”

Section: Biotransformation Of Macroalgae Polysaccharidesmentioning

confidence: 99%

Recent Advances in Bioutilization of Marine Macroalgae Carbohydrates: Degradation, Metabolism, and Fermentation

Zheng

Yang

et al. 2022

J. Agric. Food Chem.

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Marine macroalgae are considered renewable natural resources due to their high carbohydrate content, which gives better utilization value in biorefineries and higher value conversion than firstand second-generation biomass. However, due to the diverse composition, complex structure, and rare metabolic pathways of macroalgae polysaccharides, their bioavailability needs to be improved. In recent years, enzymes and pathways related to the degradation and metabolism of macroalgae polysaccharides have been continuously developed, and new microbial fermentation platforms have emerged. Aiming at the bioutilization and transformation of macroalgae resources, this review describes the latest research results from the direction of green degradation, biorefining, and metabolic pathway design, including summarizing the the latest biorefining technology and the fermentation platform design of agarose, alginate, and other polysaccharides. This information will provide new research directions and solutions for the biotransformation and utilization of marine macroalgae.

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“…Clostridium sp. strain NJ4 produced 12.56 g/L butanol using red seaweed hydrolysate, which contained 43.18 g/L of glucose after being pretreated with acid and concentration (Jiang et al, 2022), while C. beijerinckii DSM-6422 produced 7.16 g/L using brown seaweed that was pretreated by an enzyme (Hou et al, 2017). In addition, other techniques were used for improved butanol production from seaweed.…”

Section: Yield Of Butanol (G/l)mentioning

confidence: 99%

Screening of Clostridium sp. for butanol production from Sato (Thai rice wine) factory wastewater

Buranaprasopchai

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The production of Sato, a Thai traditional beverage, yields Sato wastewater (STW), which contains residual rice constituents with high chemical and biological oxygen demands and so forms an environmental problem. In addition, STW often smells rotten from the formation of hydrogen sulfide. To solve this problem, the composition of STW was analyzed for its physiological and chemical characteristics to evaluate its potential as a substrate for ABE fermentation using Clostridium spp., which has never been reported for use in acetone-butanol-ethanol (ABE) fermentation before. Solvent-producing Clostridium isolates were obtained from environmental sources in Thailand, and that producing the highest butanol level (isolate CUEA02 from the sludge of a biodiesel plant) was selected. The butanol production was 8.32 ? 0.08 g/L in flask fermentation under an initial pH of 6.5, 35 ?C, 10% (v/v) inoculum size, and 50 g/L of glucose. Whole-genome sequencing analysis was used to identify this strain and its ability to use different carbon sources for ABE fermentation was evaluated. Isolate CUEA02 was closely related to Clostridium beijerinckii with an average nucleotide identity of 95.14% and could grow and produce butanol in various carbon sources, especially starch and carboxymethyl cellulose. The STW was diluted 1:10, 1:20, 1:30, and 1:40�for ABE production by CUEA02, where the obtained butanol yield (0.23 g/g) under an anaerobic condition was highest with the 1:10�diluted STW. To improve the butanol production, the initial pH was adjusted from 4.75 to 6.5, resulting in a 2.13-fold enhanced butanol yield (0.49 g/g). Furthermore, adding yeast extract at 2 g/L enhanced butanol production in STW utilizing C. beijerinckii CUEA02 by up to 29%.

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High butanol production from galactose riched red seaweed Gracilaria lemaneiformis by solventogenic Clostridium sp. strain NJ4

Cited by 7 publications

References 27 publications

EVALUATION OF OIL PALM FROND FOR BIOBUTANOL PRODUCTION BY DIFFERENT Clostridia SPECIES

EVALUATION OF OIL PALM FROND FOR BIOBUTANOL PRODUCTION BY DIFFERENT Clostridia SPECIES

Recent Advances in Bioutilization of Marine Macroalgae Carbohydrates: Degradation, Metabolism, and Fermentation

Screening of Clostridium sp. for butanol production from Sato (Thai rice wine) factory wastewater

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