Clostridium sp. Strain NJ4: A Promising Solventogenic Strain for Butanol Production from Jerusalem Artichoke through Consolidated Bioprocessing

Abstract

Clostridium sp. strain NJ4 was successfully isolated and characterized, which exhibited a wide carbon utilization spectrum, including glucose, fructose, fructooligosaccharide, and Jerusalem artichoke. In comparison to 14.71 and 15.81 g L–1 butanol produced from glucose and fructose, a similar butanol production was obtained by strain NJ4 from fructooligosaccharide and Jerusalem artichoke without supplementation of exogenous inulinases, owing to its efficient expression of levanase and fructan β-fructosidase. After the process optimization, 13.25 g L–1 butanol was directly produced from 90 g L–1 Jerusalem artichoke, indicating that strain NJ4 could be a promising candidate for butanol production from Jerusalem artichoke through consolidated bioprocessing (CBP) at a large scale. In addition, extra supplementation of sodium butyrate could improve butanol and acetone–butanol–ethanol titers to 14.35 and 24.75 g L–1. To the best of our knowledge, this represents the highest butanol production from Jerusalem artichoke through CBP.

Comprehensive evaluation for the one-pot biosynthesis of butyl acetate by using microbial mono- and co-cultures

“…C. acetobutylicum NJ4 was isolated and stored by our lab [ 23 , 24 ]. A. succinogenes 130z (Δ pflA ) was obtained by knocking out pflA from A. succinogenes 130z (ATCC 55618), which can efficiently produce acetic acid from glucose [ 22 ].…”

“…Concentrations of acetic acid and glucose were measured by high-performance liquid chromatography (HPLC; UltiMate 3000 HPLC system; Dionex, Sunnyvale, CA) using an ion-exchange chromatographic column (Bio-Rad Aminex HPX‐87H column) at a wavelength of 215 nm on a UVD 170U ultraviolet detector [ 24 ]. Butanol and butyl acetate were detected by gas chromatography (GC-2010, Shimadzu Scientific Instruments, Japan) equipped with an InterCap WAX column (0.25 mm × 30 m, GL Sciences Inc., Japan) and a flame ionization detector (FID) [ 7 ].…”

“…Previously, we have genetically constructed Actinobacillus succinogenes 130z (Δ pflA ), which could efficiently produce acetic acid from glucose [ 22 ]. Furthermore, one butanol hyper producer of C. acetobutylicum NJ4 was isolated and stored by our lab [ 23 , 24 ]. Accordingly, three proof-of-principle strategies for butyl acetate production were comprehensively evaluated, including A. succinogenes 130z (Δ pflA ) fermentation with the exogenous supplementation of butanol, C. acetobutylicum NJ4 fermentation with the exogenous supplementation of acetic acid, and microbial co-culture fermentation composed of A. succinogenes 130z (Δ pflA ) and C. acetobutylicum NJ4.…”

Production of butanol from lignocellulosic biomass: recent advances, challenges, and prospects

“…1 Butanol, a useful biofuel and important platform chemical, has attracted great interest due to its excellent fuel properties (high energy density, excellent mixability, low volatility and corrosiveness). 2,3 However, high substrate cost, solvent toxicity, low butanol titre and poor productivity make butanol fermentation expensive for large-scale practical applications. 4 Traditionally, butanol has been produced via acetone-butanol-ethanol (ABE) fermentation from sugars or starch as substrates, but the cost of substrates ($60% of the total cost of ABE fermentation) signicantly decreased the competitiveness of ABE fermentation, so much so that biobutanol produced by Clostridium cannot compete with petrochemical butanol.…”

Modular Metabolic Engineering of Bacillus licheniformis for Efficient 2,3-Butanediol Production by Consolidated Bioprocessing of Jerusalem Artichoke Tubers

“…Alternatively, a consolidated bioprocessing (CBP) strategy offers a promising approach for inulin-based biorefinery, which systematically integrates inulinase production, inulin hydrolysis and fermentation in one-pot using a single microorganism. 19 Unfortunately, the optimal hydrolysis temperature (45−55 °C) of most inulinases highly conflicts with the optimal fermentation temperature (30−37 °C) of most BDO producers, which is undesirable for CBP. 20 Bacillus licheniformis is an attractive chassis for biomanufacturing due to its non-pathogenicity, excellent robustness, and genetic tractability.…”