Study on the interaction of graphene oxide‑silver nanocomposites with bovine serum albumin and the formation of nanoparticle-protein corona

Xu, Xiangyu; Mao, Xuyan; Wang, Yunfei; Li, Dandan; Du, Zhongyu; Wu, Weihua; Jiang, Liang; Yang, Jie; Li, Jianjun

doi:10.1016/j.ijbiomac.2018.05.043

Cited by 48 publications

(30 citation statements)

References 45 publications

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“…In the XRD pattern of Figure 2, the diffraction peaks appear at 38.08°, 44.16°, 64.44°, and 77.44°, corresponding to the (111), (200), (220), and (311) diffraction peaks of fcc Ag [26]. The results were basically consistent with the literature, indicating that the graphene oxide added with AgNO 3 is reduced by glucose to form graphene-silver nanoparticles.…”

Section: Resultssupporting

confidence: 86%

“…To further investigate the interaction of BSA and rGO-AgNPs, the surface morphology of the system was analyzed by SPM, which is a very reliable tool for biological applications [26,31,32]. The SPM image clearly showed that BSA molecules adsorbed onto mica ( Figure 5(a)).…”

Section: Spm Analysis Of the Interaction Of Rgo-agnps With Bsamentioning

confidence: 99%

“…The fluorescence lifetime of BSA is about 10 -8 s. F 0 and F are the fluorescence intensities of BSA in the absence and presence of rGO-AgNPs, respectively. ½Q is the concentration of the quencher [26]. Based on fluorescence data obtained at two different temperatures (303 K and 308 K, see Figure 6), the Stern-Volmer plots are shown in Figure 7, and the corresponding values are listed in Table 1.…”

Section: Spm Analysis Of the Interaction Of Rgo-agnps With Bsamentioning

confidence: 99%

“…Nanoparticles with special properties of graphene and AgNPs are effective medical nanomaterials, which have attracted wide interest [18][19][20][21][22][23]. Therefore, the formation of nanoparticle-protein corona of graphene oxide-silver nanoparticles with BSA has been investigated in our previous work [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of Reduced Graphene Oxide-Ag Nanocomposites and Analysis on the Interaction with BSA

Zhang

Yang

et al. 2019

Journal of Nanomaterials

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Graphene is an excellent platform to support and stabilize silver nanoparticles (AgNPs). The reduced graphene oxide-silver nanoparticles (rGO-AgNPs) were synthesized by the chemical reduction method and characterized by using ultraviolet-visible (UV-vis) absorption, transmission electron microscopy (TEM), X-ray diffractometer (XRD), and scanning probe microscopy (SPM). The binding reaction of rGO-AgNPs with bovine serum albumin (BSA) was investigated by using fluorescence spectrometry and SPM. As the concentration of AgNPs increased, the fluorescence spectrum was quenched, and the quenching process of rGO-AgNPs and BSA was static quenching. Thermodynamic parameters of the absorption process were evaluated at different temperatures, and the negative values of Gibbs free energy (ΔG) showed that this process was spontaneous. The main type of interaction was hydrophobic interaction according to the values of changes in standard enthalpy (ΔH) and entropy (ΔS). In addition, the morphology changes of proteins interacting with nanomaterials were detected by SPM.

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Section: Resultssupporting

confidence: 86%

Section: Spm Analysis Of the Interaction Of Rgo-agnps With Bsamentioning

confidence: 99%

Section: Spm Analysis Of the Interaction Of Rgo-agnps With Bsamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fabrication of Reduced Graphene Oxide-Ag Nanocomposites and Analysis on the Interaction with BSA

Zhang

Yang

et al. 2019

Journal of Nanomaterials

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“…The hydrodynamic size in the cell medium was higher than that in pure water. A possible reason is that GO can combine with proteins, antibiotic, and other biomolecules in biological fluids leading to the formation of "nanoparticle-protein corona" [41]. Even though FBS protein in cell culture medium can slightly improve the dispersion stability of GO [42,43], GO showed to be very stable in pure water, indicating GO was more stable in pure water than in culture medium and less likely to cause aggregation.…”

Section: Discussionmentioning

confidence: 99%

The interrupted effect of autophagic flux and lysosomal function induced by graphene oxide in p62-dependent apoptosis of F98 cells

Zhang

Feng

et al. 2020

J Nanobiotechnol

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Background: Graphene oxide (GO) nanoparticles (NPs) have been widely applied in various fields, especially in biomedical applications. Extensive studies have suggested that GO can pass through the blood-brain barrier (BBB) and induce abnormal autophagy and cytotoxicity in the central nervous system (CNS). However, the effect and specific mechanism of GO on astrocytes, the most abundant cells in the brain still has not been extensively investigated. Results: In this study, we systematically explored the toxicity and mechanism of GO exposure in the rat astrogliomaderived F98 cell line using molecular biological techniques (immunofluorescence staining, flow cytometry and Western blot) at the subcellular level and the signaling pathway level. Cells exposed to GO exhibited decreased cell viability and increased lactate dehydrogenase (LDH) release in a concentration-and time-dependent manner. GO-induced autophagy was evidenced by transmission electron microscopy (TEM) and immunofluorescence staining. Western blots showed that LC3II/I and p62 were upregulated and PI3K/Akt/mTOR was downregulated. Detection of lysosomal acidity and cathepsin B activity assay indicated the impairment of lysosomal function. Annexin V-FITC-PI detection showed the occurrence of apoptosis after GO exposure. The decrease in mitochondrial membrane potential (MMP) with an accompanying upregulation of cleaved caspase-3 and Bax/Bcl-2 further suggested that endogenous signaling pathways were involved in GO-induced apoptosis. Conclusion: The exposure of F98 cells to GO can elicit concentration-and time-dependent toxicological effects. Additionally, increased autophagic response can be triggered after GO treatment and that the blocking of autophagy flux plays a vital role in GO cytotoxicity, which was determined to be related to dysfunction of lysosomal degradation. Importantly, the abnormal accumulation of autophagic substrate p62 protein can induce capase-3-mediated apoptosis. Inhibition of abnormal accumulation of autophagic cargo could alleviate the occurrence of GO-induced apoptosis in F98 cells.

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Disinfector‐Assisted Low Temperature Reduced Graphene Oxide‐Protein Surgical Dressing for the Postoperative Photothermal Treatment of Melanoma

Yang

Teijlingen

et al. 2022

Adv Funct Materials

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Materials that combine the functionalities of both of proteins and graphene are of great interest for the engineering of biosensing, drug delivery, and regenerative devices. Graphene oxide (GO) offers an opportunity to design GO-protein interactions but the need for harsh reduction processes to enable GO photoexcitation remains a limitation. A disinfector-assisted low temperature method to reduce GO-protein materials and fabricate surgical dressings with tuneable photothermal efficiency and bioactive properties for the postoperative treatment of melanoma is reported. The approach harnesses the capacity of 70% ethanol to penetrate the protein shell of microorganisms to infiltrate GO-protein complexes and reduce GO at low temperature (85 °C) while maintaining the material structure and bioactivity. Both experiments and coarse-grained simulations are used to describe the reduction process and assess the material properties. In vitro and in vivo validation revealed the capacity of the dressings to prevent tumor recurrence and promote healing after tumor resection.

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Study on the interaction of graphene oxide‑silver nanocomposites with bovine serum albumin and the formation of nanoparticle-protein corona

Cited by 48 publications

References 45 publications

Fabrication of Reduced Graphene Oxide-Ag Nanocomposites and Analysis on the Interaction with BSA

Fabrication of Reduced Graphene Oxide-Ag Nanocomposites and Analysis on the Interaction with BSA

The interrupted effect of autophagic flux and lysosomal function induced by graphene oxide in p62-dependent apoptosis of F98 cells

Disinfector‐Assisted Low Temperature Reduced Graphene Oxide‐Protein Surgical Dressing for the Postoperative Photothermal Treatment of Melanoma

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