Fabrication of Reduced Graphene Oxide-Ag Nanocomposites and Analysis on the Interaction with BSA

Zhang, Huali; Li, Wen; Yang, Li‐Tao; Liu, Jun; Wang, Yunfei; Mao, Xuyan; Wang, Jing; Xu, Xiangyu

doi:10.1155/2019/2707909

Cited by 9 publications

(2 citation statements)

References 34 publications

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“…Similar findings were also observed in a study by Lin-jun et al [32], where a similar nanocomposite was synthesised through a reduction process using a facile one pot process. The shoulder at approximately 300 nm is attributed to electronic π-π * transitions of C-C aromatics [33] and the pear extract spectrum indicates an absorption band at 270 nm characteristic of polyphenols and flavonoids [34,35]. The location and the shape of the GO|Ag-NPs absorption band is related to the particle size and distribution where, in this study, the low SPR band is attributed to the fact that small nanocomposites were developed as indicated below.…”

Section: Optical and Structural Properties Of Go|ag-nps Nanocompositesmentioning

confidence: 53%

Green Method Synthesised Graphene-Silver Electrochemical Nanobiosensors for Ethambutol and Pyrazinamide

et al. 2020

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A novel nanobiosensor was constructed with graphene oxide (GO) sheets coupled to pear extract-based green-synthesised silver nanoparticles (Ag-NPs) to which cytochrome P450-2D6 (CYP2D6) enzyme was attached. The biosensor was applied in the electrochemical detection of the tuberculosis (TB) treatment drugs, ethambutol (EMB) and pyrazinamide (PZA). The surface morphology of the green-synthesised nanocomposites was studied by performing High-Resolution Transmission Electron Microscopy (HR-TEM) and High-Resolution Scanning Electron Microscopy (HR-SEM). Fourier Transform Infrared Spectroscopy (FTIR) and Raman Spectroscopy were used for structural analysis, while Ultraviolet Visible (UV-Vis) Spectroscopy was used in the optical characterisation of the nanocomposite material. Electrochemical studies on glassy carbon electrode (GCE), which were done by Cyclic Voltammetry (CV), showed that the GO|Ag-NPs||GCE electrode was highly conductive, and thereby indicating its suitability as a platform for nanobiosensor development. The non-toxic and low-cost green GO|Ag-NPs|CYP2D6||GCE nanobiosensor was used to determine EMB and PZA. The very low limit of detection (LOD) values of the biosensor for EMB (0.2962 × 10−2 nM, S/N = 3) and PZA (0.897 × 10−2 nM, S/N = 3) demonstrate that the green nanobiosensor is more sensitive than other biosensors reported for EMB and PZA.

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Section: Optical and Structural Properties Of Go|ag-nps Nanocompositesmentioning

confidence: 53%

Green Method Synthesised Graphene-Silver Electrochemical Nanobiosensors for Ethambutol and Pyrazinamide

et al. 2020

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“…As shown in Fig. 2 a , GO displays a maximum absorption peak centred at 230 nm and a shoulder peak at about 300 nm [43]. After AgNPs successfully bound to the surface of GO and reduction by glucose, a new peak at about 412 nm can be clearly observed.…”

Section: Resultsmentioning

confidence: 99%

Studies on binding of single‐stranded DNA with reduced graphene oxide–silver nanocomposites

Yang

Wang

et al. 2020

IET nanobiotechnol.

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The binding reaction of reduced graphene oxide-silver nanocomposites (rGO-AgNCs) with calf thymus singlestranded DNA (ssDNA) was studied by ultraviolet-visible absorption, fluorescence spectroscopy and circular dichroism (CD), using berberine hemisulphate (BR) dye as a fluorescence probe. The absorbance of ssDNA increases, but the fluorescence intensity is quenched with the addition of rGO-AgNCs. The binding of rGO-AgNCs with ssDNA was able to increase the quenching effects of BR and ssDNA, and induce the changes in CD spectra. All of the evidence indicated that there was a relatively strong interaction between ssDNA and rGO-AgNCs. The data obtained from fluorescence experiments revealed that the quenching process of ssDNA caused by rGO-AgNCs is primarily due to complex formation, i.e. static quenching. The increasing trend of the binding equilibrium constant (Ka) with rising temperature indicated that the binding process was an endothermic reaction. The calculated thermodynamic parameters showed that the binding process was thermodynamically spontaneous, and hydrophobic association played predominant roles in the binding of ssDNA to the surface of rGO-AgNCs.

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Exploring Nano‐Protein Corona Dynamics: Tracing the Hard‐to‐Soft Corona Transition with Trypsin and Graphene Oxide in a Silver Nanocomposite Model

Sharma,

Yadav,

Kumari

et al. 2024

ChemistrySelect

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In this work, the adsorption of Trypsin on synthesized Silver (Ag), Graphene Oxide: Silver (GO: Ag) nanocomposite (2:1), and Graphene Oxide (GO) nanoparticles (NPs) and, their effect on the conformation of Trypsin was studied by various spectroscopic and microscopic techniques to understand the transition of nano Protein Corona (PC) from “hard” to “soft.” The results showed that the adsorption of Trypsin on synthesized NPs followed the Freundlich adsorption isotherm and pseudo‐second‐order kinetics. The conformational changes that occurred in Trypsin upon interaction with synthesized NPs were investigated by using Circular Dichroism (CD), Fluorescence, and Fourier Transform Infrared (FTIR) spectroscopy. The morphological investigation of nano PC using High‐Resolution Transmission Electron Microscopy (HR‐TEM) and Atomic Force Microscopy (AFM) indicated the formation of hard, semi‐soft, and soft nano PC in the presence of Ag, GO: Ag, and GO NPs, respectively. The negative value of the thermodynamic parameters ΔG, ΔH, and ΔS in all three cases suggests the reaction to be exothermic and spontaneous. The van der Waals interaction and hydrogen bonding are the major forces responsible for binding Trypsin on the surface of Ag, GO: Ag, and GO. The catalytic efficiency of trypsin in the absence and presence of NPs was examined by performing the protein assay showing the highest catalytic activity of pure Trypsin compared with trypsin‐NPs constructs. Our findings provide useful information for the applications of GO‐based nanocomposites for various biological applications.

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Fabrication of Reduced Graphene Oxide-Ag Nanocomposites and Analysis on the Interaction with BSA

Cited by 9 publications

References 34 publications

Green Method Synthesised Graphene-Silver Electrochemical Nanobiosensors for Ethambutol and Pyrazinamide

Green Method Synthesised Graphene-Silver Electrochemical Nanobiosensors for Ethambutol and Pyrazinamide

Studies on binding of single‐stranded DNA with reduced graphene oxide–silver nanocomposites

Exploring Nano‐Protein Corona Dynamics: Tracing the Hard‐to‐Soft Corona Transition with Trypsin and Graphene Oxide in a Silver Nanocomposite Model

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