The involvement of phosphorylation of myosin phosphatase targeting subunit 1 (<scp>MYPT</scp>1) and <scp>MYPT</scp>1 isoform expression in <scp>NO</scp>/<scp>cGMP</scp> mediated differential vasoregulation of cerebral arteries compared to systemic arteries

Lubomirov, Lubomir T.; Papadopoulos, Symeon; Filipova, Dilyana; Baransi, Saher; Todorović, Dajana; Lake, Philipp; Metzler, Doris; Hilsdorf, S.; Schubert, Rudolf; Schroeter, Mechthild M.; Pfitzer, Gabriele

doi:10.1111/apha.13079

Cited by 20 publications

(34 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…; Lubomirov et al. ). This effect was attributed to NO release and an increase in cGMP concentration.…”

Section: Resultsmentioning

confidence: 97%

“…; Lubomirov et al. ). All these data support the view that phosphorylation at T853 might serve as important mechanosensor involved in normotensive and pathological response of the vasculature.…”

Section: Discussionmentioning

confidence: 97%

“…Ten minutes after temperature stabilization the vessels were stretched stepwise to their optimal (IC 90 ) length determined as in Mulvany and Halpern ()and Lubomirov et al. (, ). Initial basal tone of murine FA was obtained after radial stretch to ~13.3 kPa in PSS, followed by a release with 10% to IC 90 (90% of internal diameter at which vessels reach wall tension of 13.3 kPa), which was kept over the whole experiment.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Augmented contractility of murine femoral arteries in a streptozotocin diabetes model is related to increased phosphorylation of MYPT1

et al. 2019

View full text Add to dashboard Cite

Diabetes mellitus (DM) is a metabolic disorder with high prevalence, and a major risk factor for macro‐ and microvascular abnormalities. This study was undertaken to explore the mechanisms of hypercontractility of murine femoral arteries (FA) obtained from mice with streptozotocin (STZ)‐induced diabetes and its relation to the phosphorylation profile of the myosin phosphatase target subunit 1, MYPT1. The immunoreactivity of MYPT1 toward phospho‐MYPT1‐T696, MYPT1‐T853, or MYPT1‐S695, used as a read out for MYPT1 phosphorylation, has been studied by Western Blotting. Contractile activity of FA from control and STZ mice has been studied by wire myography. At basal conditions (no treatment), the immunoreactivity of MYPT1‐T696/T853 was ~2‐fold higher in the STZ arteries compared with controls. No changes in MYPT1‐T696/853 phosphorylation were observed after stimulation with the Thromboxan‐A2 analog, U46619. Neither basal nor U46619‐stimulated phosphorylation of MYPT1 at S695 was affected by STZ treatment. Mechanical distensibility and basal tone of FA obtained from STZ animals were similar to controls. Maximal force after treatment of FA with the contractile agonists phenylephrine (10 μmol/L) or U46619 (1 μmol/L) was augmented in the arteries of STZ mice by ~2‐ and ~1.5‐fold, respectively. In summary, our study suggests that development of a hypercontractile phenotype in murine FA in STZ diabetes is at least partially related to an increase in phosphorylation of MLCP at MYPT1‐T696/853. Interestingly, the phosphorylation at S695 site was not altered in STZ‐induced diabetes, supporting the view that S695 may serve as a sensor for mechanical activity which is not directly involved in tone regulation.

show abstract

“…; Lubomirov et al. ). This effect was attributed to NO release and an increase in cGMP concentration.…”

Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Augmented contractility of murine femoral arteries in a streptozotocin diabetes model is related to increased phosphorylation of MYPT1

et al. 2019

View full text Add to dashboard Cite

show abstract

“…In the work of Lubomirov, the role of phosphorylation of MYPT1 and related isoforms are involved in cGMP sensitivity in arteries. The authors apply a number of pharmacological interventions, force measurements and methods of protein biochemistry to address this question.…”

mentioning

confidence: 99%

Recent advances in hypertension research

Mrowka

2019

Acta Physiologica

View full text Add to dashboard Cite

“…Despite its underlying principle of vascular occlusion, hypoperfusion and ischaemic organ failure, CVD characteristics differ between vascular beds. Both cerebral and cardiac arteries differ significantly in vasomotor function, CVD development and progression.…”

mentioning

confidence: 99%