295 Immunohistochemical examination of the cellular infiltrate in asthmatic bronchi

Poston, R.N.; Chanez, Pascal; Lacoste, J.-Y.; Litchfield, T.M.; Lee, T.H.; Bousquet, Philippe‐Jean

doi:10.1016/0091-6749(91)91578-h

Cited by 5 publications

(3 citation statements)

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“…Carlson et al (28) have reported that birch pollen allergic subjects with asthma have increased secretion of MPO from isolated neutrophils during the pollen season. By contrast, Bousquet et a1 (10) were unable to find increased levels of MPO in BAL fluid of patients with asthma, which supports the results of a study of bronchial biopsies which failed to reveal any increase in the number of neutrophils in the bronchi of asthmatic subjects (29).…”

Section: Discussionmentioning

confidence: 83%

Eosinophil cationic protein, myeloperoxidase and tryptase in children with asthma and atopic dermatitis

Kristjánsson

Shimizu

Strannegård

et al. 1994

Pediatric Allergy Immunology

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Serum levels of eosinophil cationic protein (ECP), myeloperoxidase (MPO), tryptase, total IgE and differential blood cell counts were studied in atopic children with: 1) moderate to severe asthma using inhaled steroids and symptom-free for the last 3 weeks (n = 13), 2) mild asthma with sporadic symptoms, using only inhaled beta 2-agonists < 3 times/week (n = 15), 3) acute asthmatic attacks admitted to hospital (n = 12), 4) mild to moderate atopic dermatitis (n = 14). Fifteen children without any history of atopy served as controls. ECP, MPO, tryptase and IgE were measured in serum by radioimmunoassays (RIA). The symptom-free children with inhaled steroids had similar median ECP and MPO values as the controls, 8.0 and 360 micrograms/l, vs. 9.0 and 310 micrograms/l, while both ECP and MPO were significantly (p < 0.001) increased in the symptom-free children without anti-inflammatory treatment, 32 and 887 micrograms/l and in those with acute asthma, 28 and 860 micrograms/l. The children with atopic dermatitis had increased ECP but normal MPO levels, 16.0 and 455 micrograms/l. Tryptase in serum was not measurable in any patient. All groups except the control group had significantly elevated total IgE levels. The results indicate that in atopic children serum ECP is a good marker of ongoing asthma or atopic dermatitis. The normal levels of ECP and MPO in the children with asthma using inhaled steroids seem to reflect successful anti-inflammatory treatment. The increased levels of ECP and MPO in the children with mild asthma and no anti-inflammatory treatment may indirectly reflect airway inflammation.

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Section: Discussionmentioning

confidence: 83%

Eosinophil cationic protein, myeloperoxidase and tryptase in children with asthma and atopic dermatitis

Kristjánsson

Shimizu

Strannegård

et al. 1994

Pediatric Allergy Immunology

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“…The second phase, which occurs in only a subset of subjects who develop Th2 memory against these inhaled antigens, involves chronic airways inflammation characterized by the presence of large numbers of activated T cells (70), which infers the presence in the airway mucosa of functionally mature APCs. It is of interest to note in this context that local airway intraepithelial production of high levels of GM-CSF is a hallmark of atopic asthma (71). It is also relevant to note recent studies that suggest that peripheral tissue DC populations comprise two distinct myeloid lineages, one of which is characterized by high endocytic activity/IL-10 production/capacity to induce naive B cell switching, and the second being specialized for T cell activation (72,73).…”

Section: Discussionmentioning

confidence: 99%

Resting Respiratory Tract Dendritic Cells Preferentially Stimulate T Helper Cell Type 2 (Th2) Responses and Require Obligatory Cytokine Signals for Induction of Th1 Immunity

Stumbles

Thomas

Pimm

et al. 1998

The Journal of Experimental Medicine

431

306

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Consistent with their role in host defense, mature dendritic cells (DCs) from central lymphoid organs preferentially prime for T helper cell type 1 (Th1)-polarized immunity. However, the “default” T helper response at mucosal surfaces demonstrates Th2 polarity, which is reflected in the cytokine profiles of activated T cells from mucosal lymph nodes. This study on rat respiratory tract DCs (RTDCs) provides an explanation for this paradox. We demonstrate that freshly isolated RTDCs are functionally immature as defined in vitro, being surface major histocompatibility complex (MHC) II lo, endocytosishi, and mixed lymphocyte reactionlo, and these cells produce mRNA encoding interleukin (IL)-10. After ovalbumin (OVA)-pulsing and adoptive transfer, freshly isolated RTDCs preferentially stimulated Th2-dependent OVA-specific immunoglobulin (Ig)G1 responses, and antigen-stimulated splenocytes from recipient animals produced IL-4 in vitro. However, preculture with granulocyte/macrophage colony stimulating factor increased their in vivo IgG priming capacity by 2–3 logs, inducing production of both Th1- and Th2-dependent IgG subclasses and high levels of IFN-γ by antigen-stimulated splenocytes. Associated phenotypic changes included upregulation of surface MHC II and B7 expression and IL-12 p35 mRNA, and downregulation of endocytosis, MHC II processing– associated genes, and IL-10 mRNA expression. Full expression of IL-12 p40 required additional signals, such as tumor necrosis factor α or CD40 ligand. These results suggest that the observed Th2 polarity of the resting mucosal immune system may be an inherent property of the resident DC population, and furthermore that mobilization of Th1 immunity relies absolutely on the provision of appropriate microenvironmental costimuli.

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“…Mast cells are often found adjacent to blood vessels and appear to be intimately related to bronchial smooth muscle. These cells were shown to release elastase and cytokines that may amplify the vaso-active phase of the allergic inflammation as well as attract inflammatory cells (25).…”

Section: Inflammation and Repair In Asthmamentioning

confidence: 99%

Asthma: a disease remodeling the airways

Bousquet

Chanez

Lacoste

et al. 1992

Allergy

242

151

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295 Immunohistochemical examination of the cellular infiltrate in asthmatic bronchi

Cited by 5 publications

References 0 publications

Eosinophil cationic protein, myeloperoxidase and tryptase in children with asthma and atopic dermatitis

Eosinophil cationic protein, myeloperoxidase and tryptase in children with asthma and atopic dermatitis

Resting Respiratory Tract Dendritic Cells Preferentially Stimulate T Helper Cell Type 2 (Th2) Responses and Require Obligatory Cytokine Signals for Induction of Th1 Immunity

Asthma: a disease remodeling the airways

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